Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Partial Purification and Characterization of Thermostable Alkaline $\beta$-Mannanase from Bacillus sp. JB-99 Suitable for Pulp Bleaching

  • VIRUPAKSHI S. (Department of Biotechnology, Gulbarga University) ;
  • BABU K. GlREESH (Department of Biotechnology, Gulbarga University) ;
  • NAIK GAJANAN R. (Department of Biotechnology, Gulbarga University)
  • Published : 2005.08.01
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Abstract

Bacillus sp. JB-99, when grown in a chemically defined medium containing lactose as a carbon source, yielded 3,860 U/ml extracellular $\beta$-mannanase, which was high compared to other examined carbon sources. Among the nitrogen sources, yeast extract enhanced the enzyme activity. The enzyme production was growth-associated. The enzyme was optimally active at $65^{\circ}C$, pH 10, and had a half-life of 190 min at $65^{\circ}C$. N-Bromosuccinamide and $AgNO_3,\;CuSO_4$, and $HgCl_2$ strongly inhibited the enzyme, whereas $Ca^{2+}$ stimulated the enzyme activity. The $\alpha$-galactosidase enzyme production was not found in any of the enzyme assays.

Keywords

References

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