Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Human ${\beta}$-Globin Second Intron Highly Enhances Expression of Foreign Genes from Murine Cytomegalovirus Immediate-Early Promoter

  • KANG MOONKYUNG (Vector Core A Inc. R&D Center) ;
  • KIM SEON-YOUNG (Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE SUKYUNG (Vector Core A Inc. R&D Center) ;
  • LEE YOUNG-KWAN (Indang Institute of Molecular Biology, Inje University) ;
  • LEE JAEHO (Indang Institute of Molecular Biology, Inje University) ;
  • SHIN HYUN-SEOCK (School of Life Science and Biotechnology, Korea University) ;
  • KIM YEON-SOO (Indang Institute of Molecular Biology, Inje University)
  • Published : 2005.06.01
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Abstract

To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human ${\beta}$-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human ${\beta}$-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.

Keywords

References

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