Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Thymidine Production by Corynebacterium ammoniagenes Mutants

  • Song, Kyung-Hwa (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kwon, Do-Young (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Sang-Yong (BioNgene Co., Ltd.) ;
  • Lee, Jung-Kul (BioNgene Co., Ltd.) ;
  • Hyun, Hyung-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • Published : 2005.06.01
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Abstract

Corynebacterium ammoniagenes ATCC 6872, which does not accumulate pyrimidine nucleoside or nucleotide, was metabolically engineered to secrete a large amount of thymidine. Characteristics of 5-fluorouracil resistance ($FU^r$), hydroxyurea resistance ($HU^r$), trimethoprim resistance ($TM^r$), thymidylate phosphorylase deficiency ($deoA^-$), inosine auxotrophy ($ino^-$), 5-fluorocytosine resistance ($FC^r$), thymidine kinase deficiency, and thymidine resistance ($thym^r$) were successively introduced into mutant strains KR3 and DY5T9-5, and shake-flask cultures were able to accumulate 408.1 mg/l and 428.2 mg/l of thymidine, respectively, as a major product. The mutant strains did not accumulate thymine at all and accumulated less than 10 mg/l of other pyrimidine nucleosides, such as cytosine, cytidine, and deoxycytidine, as byproducts.

Keywords

References

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