Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Assessment of Characteristics of Biofilm Formed on Autotrophic Denitrification

  • JANG AM (Department of Civil and Environmental Engineering, University of Cincinnati) ;
  • BUM MINSU (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST)) ;
  • KIM SUNGYOUN (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST)) ;
  • AHN YEONGHEE (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST)) ;
  • KIM IN S (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST)) ;
  • BISHOP PAUL L (Department of Civil and Environmental Engineering, University of Cincinnati)
  • Published : 2005.06.01
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Abstract

A pilot-scale sulfur particle autotrophic denitrification (SPAD) process for the treatment of municipal wastewater was operated for 10 months at Shihwa, Korea, and higher than $90\%\;NO^{-}_{3}-N$ removal efficiency was observed. Plate counting showed that the lower part of the denitrifying column reactor had the most autotrophic denitrifiers. The biofilm thickness formed on sulfur particles from the SPAD reactor was approximately $25-30\;{\mu}m$, measured by DAPI (4,6-diamidino-2-phenylindole) staining. The presence of bacteria inside the highly porous sulfur particle was also monitored by SEM observation of the internal surfaces of broken sulfur particles. Biofilm extracellular polymeric substances (EPS) analysis showed that the ratio of carbohydrate to protein decreased with the reactor heights at which biofilm-formed sulfur particles were obtained.

Keywords

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