Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Improvement of Catechin Productivity in Callus Cultures of Camellia sinensis Leaves

차나무 잎의 캘러스 배양을 통한 카테킨류의 생산성 개선

  • Oh, Soon-Ja (Department of Life Science & Research Institute for Basic Sciences, Cheju National University) ;
  • Koh, Seok-Chan (Department of Life Science & Research Institute for Basic Sciences, Cheju National University)
  • 오순자 (제주대학교 생명과학과, 기초과학연구소) ;
  • 고석찬 (제주대학교 생명과학과, 기초과학연구소)
  • Published : 2005.06.01
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Abstract

The effects of thiamine-HCl or polyvinylpyrrolidone (PVP) on growth and catechin contents of calli from Camellia sinensis leaves were investigated to improve catechin productivity in callus cultures on the selective medium. The growth of calli was great on the proliferation medium (the MS medium with 0.2 mg/L 2,4-D and 1.0 mg/L TDZ) supplemented with $20{\sim}30\;mg/L$ thiamine-HCl. Although the caffeine content was low in calli compared to young leaves, the total content of catechins was high in calli. Particularly,(-)-epicatechin (EC) which not detected in young loaves was also detected in calli on the propagation medium containing thiamine-HCl or PVP. In addition, the (-)-epicatechingallate (ECG) content was significantly higher in calli than in young leaves. In conclusion, the proliferation medium supplemented with 30 mg/L thiamine-HCl as a vitamin source seemed to be optimal condition for the growth and catechin production in callus culture.

차나무 카테킨류의 생산성을 높이기 위하여 차나무 잎의 캘러스 배양시 thiamine-HCl과PVP의 효과를 조사하였다. 캘러스의 생장은 20 mg/L thiamine-HCl이 포함된 캘러스증식배지(0.2 mg/L 2,4-D와 1.0 mg/L TDZ가 첨가된 MS 배지)에서 가장 왕성하여 대조구에 비해 1.7배의 생장을 보였다. 배양된 캘러스의 카페인 함량은 신초에 비해 $10\%$이하로 낮아졌고 총 카테킨류 함량은 40 mg/L thiamine-HCl에서 가장 높아 신초의 1.6배까지 증가하였다. 특히 신초에서는 검출되지 않았던 (-)-epicatechin(EC)이 thiamine-HCl 또는 PVP를 처리하여 배양하였을 경우 모든 처리에서 검출되었으며, (-)-epicatechingallate(ECG)는 신초에서 보다 4.8배나 높은 함량을 나타내었다. 캘러스 생장과 고효율의 카테킨 생산을 고려했을 때 30 mg/L thiamine-HCl이 포함된 배지가 최적의 배양조건으로 판단된다.

Keywords

References

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