Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Isolation of Gglutatihone S-Ttransferase(ClGST) Gene from Codonopsis lanceolata

더덕에서 Glutathione S-transferase (ClGST) 유전자의 분리

  • Kim Jin-Ju (Dept. of Oriental Medicinal Materal and Processing, College of Life Science Kyung Hee University) ;
  • Yang Deok-Chun (Dept. of Oriental Medicinal Materal and Processing, College of Life Science Kyung Hee University)
  • 김진주 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 양덕춘 (경희대학교 생명과학대학 한방재료가공학과)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A cDNA clone homologous to glutathione S-transferase gene was isolated and characterized from Codonopsis lanceolata(ClGST). The ClGST is 761 nucleotides long and has an open reading frame of 522 bp with a deduced amino acid sequence of 173 residues. The ClGST shows meaning homology to A. thaliana(AAC63629) $71\%$, C. chinense(CAI51314) $73\%$, E. esula(AAE65767) $75\%$, H. muticus(CAA55039) $70\%$, N. plumbaginifolia(CAA96431) $77\%$, S. commersonii(AAB65163).

더덕 뿌리에서 유래한 EST cDNA library로부터 GST(glutatione S-transferase)유전자와 높은 상동성을 나타내는 full clone cDNA를 얻었다. 더덕의 GST(glutatione S-transferase), CIGST은 761 bp의 cDNA로 173개의 아미노산을 코딩하는 522 bp의 ORF를 가지고 있으며, A. thaliana(AAC63629) $71\%$, C. chinense(CAI51314) $73\%$, E. esula(AAE65767) $75\%$, H. muticus(CAA55039) $70\%$, N. plumbaginifolia(CAA96431) $77\%$, S. commersonii(AAB65163) $76\%$등 다른 식에서 밝혀져 있는 GST(glutatione S-transferase)와 유의한 상동성을 나타내였다.

Keywords

References

  1. Altschul, S F., W. Gish, W. Miller, E W. Myers, and D.J.Lipman. 1990. Basic local aligment search tool. J. Mol BioI 215 : 377-403-410
  2. Morris, P.C., A. Kumar, D.J. Bowles. and abscisic acid regulate the expression of the Em gene of wheat. Eur. J Biochem. 190 : 625-630 https://doi.org/10.1111/j.1432-1033.1990.tb15618.x
  3. Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plant physiol. 107 : 1-7 https://doi.org/10.1104/pp.107.1.1
  4. Allen RD, Sen-Gupta A, Webb RP, Holaday AS (1994) Protection of endogenous enzyme. In Biology and Medicine. Asada K, Yosikawa T(eds). Exerpta Medica, Amsterdam. 321-322
  5. Coles B, Ketterer B(1990) The role of glutatione and glutationee S-transferase in chemical carcinogenesis. CRC Crit. Rev. Biochem. 25 : 47-70 https://doi.org/10.3109/10409239009090605
  6. Mannervik B(1985) The isozymes of glutatione Stransferase. Adv. Enzymol. Relat Mol. Biol. 57 : 357-417
  7. Pickett CB, Lu AY.(1989) glutatione S-transferase: gene structure, regulation, and biological fuction. Annu. Rev. Biochem. 59 : 61-86 https://doi.org/10.1146/annurev.bi.59.070190.000425
  8. Anderson, M.P. and Gronwald, J.W.(1991). Atrazine resistance in a velvetleaf(Abutilon theophrasti) biotype due to enhanced glutatione S-transferase activity. Plant Physiol. 96 : 104-109 https://doi.org/10.1104/pp.96.1.104
  9. Lamoureeux, G. L. and Rusness, D. G.(1986). Tridiphane [2-<3,5-dichlorophenyl>-2(2, 2.2-trichloro-ethyl) oxirane] an atrazine synergist : Enzymatic conversion to a potent glutatione Stransferase inhibitor, Pestic, Biochem. Physiol. 26 : 323-342
  10. Dean J. V., Gwronald, J. W., and Eberlain,. C. V. (1990). Induction of glutatione S-transferase isozymes in sorghum by herbicide antidotes. Plant Physiol. 92 : 467-473 https://doi.org/10.1104/pp.92.2.467
  11. Dean, J. V., Gronwald. J. W., and Anderson, M.P. (1991) glutatione S-transferase activity in nontreated and CGA-154281-treated maize shoots. N. Naturforsch. 46 : 850-855
  12. Gronwald, J. W.(1989). Influence of herbicide safeners on herbicide metabolism. In : Crop safeners for herbicides( K. K. Hatzios and R. E. Hoagland, ed). pp. 103-128. Academic Press
  13. Gronwald, J. W., Fuerst, E. P., Eberlein, C. V., and Egli, M. A.(1987). Effect ofof herbicide antidoters on glutatione content and glutatione S-transferase activity of sorghum shoots
  14. Takahashi, Y. Nagata, T.(1992). par B : An auxinregulated gene encoding glutatione S-transferase. Prpc. Natl. Acad. Sci. USA. 89 : 56-59
  15. Stephenson, : G. R., Ali, A., and Ashton, F. M.(1983) Influnce of herbicides and antidotes on glutatione levels of maize seedings. In : presticide chemistry human welfare and the environment(J. Miyamoto and p.c. kearny, ed). Vol3. pp.219-224. Pergamon
  16. Chun, J. C. and Ma, S.Y. (1994). Investigation of herbicide safeners and its mode of safening action. I. Effect of N-(4-chlorophenyl) maleimide on metolachlor absorption and metabolism
  17. Dauteman, W. C. (1994) Introduction to biochemical toxicity. pp.542-577. Detoxication metabolisms in insect
  18. Hughes, P. B. and D. A. Raftos(1985) Genetics of an esterase associated with resistance to organophosphorus insecticides in the sheep blow fly, Lucilia cuprina (Wiedemann) (Diptera:Calliphoridae). Bull, Ent. Res. 75 : 535 https://doi.org/10.1017/S0007485300014632
  19. 송승석, 오홍규, Naoki Motoyama (1993) Carboxyl esterase S. 의 살충제 포장 저항성도의 계절적 변동. 한국응용곤충학회지 32(3) : 348-353