Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

  • Kim, Mu-Chan (Department of Marine Environmental Engineering, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Gyeongsang National University)
  • 발행 : 2005.06.01
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초록

Two distinct CD3 homologue genes, $CD3\gamma/\delta\;and\;CD\varepsilon$, were isolated from a olive flounder leukocyte cDNA library and a BAC library. $CD3\gamma/\delta$ consisted of 961 bp encoding 178 amino acid residues, and $CD3\varepsilon$ consisted of 1006 bp encoding 164 amino acid residues. When compared with other known CD3 peptide sequences, the most conserved region of the two olive flounder CD3 chain peptides are the cytoplasmic domain and the least conserved are the extracellular domain. A phylogenetic analysis based on the deduced amino acid sequence grouped the two olive flounder CD3 sequences with $CD3\varepsilon$ and $CD3\gamma/\delta$, respectively. The olive flounder CD3 cluster (consisting of $CD3\varepsilon\;and\;CD3\gamma/\delta$) spans only 10.4 kb. The $CD3\varepsilon\;and\;CD3\gamma/\delta$ genes are oppositely transcribed only 3.8 kb apart. Both olive flounder CD3 genes have five exons. The two olive flounder CD3 genes were predominantly expressed in PBLs, kidney, spleen, and gills.

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참고문헌

  1. Bernot, A. and C. Auffray. 1991. Primary structure and ontogeny of an avian CD3 transcript. Proc. Natl. Acad. Sci. USA, 88, 2550-2554
  2. Bertram, E.M., R.G. Wilkinson, B.A. Lee, A.R. Jilbert and I. Kotlarski. 1996. Identification of duck T lymphocytes using an anti-human T cell (CD3) antiserum. Vet. Immunol. Immunopathol., 51, 353-363 https://doi.org/10.1016/0165-2427(95)05528-2
  3. Boris, Y.A., V.G. Sergei, M.N. Alexander, Y.M. Ludmila and Y.T. Alexander. 2000. CD3$\varepsilon$ homologues in the chondrostean fish Acipenser ruthenus. Immunogenetics, 51, 1012-1020 https://doi.org/10.1007/s002510000236
  4. Boyer, C., N. Auphan, F. Luton, J.M. Malburet, M. Barad, J.P. Bizozzero, H. Reggio and A.M. Schmitt-Verhulst. 1991. T cell receptor/CD3 complex internalization following activation of a cytolytic T cell clone: evidence for a protein kinase C-independent staurosporine-sensitive step. Eur. J. Immunol., 21, 1623-1634 https://doi.org/10.1002/eji.1830210707
  5. Buferne, M., F. Luton, F. Letourneur, A. Hoeveler, D. Couez, M. Barad, B. Malissen, A.M. Schmitt-Verhulst and C. Boyer. 1992. Role of CD3 delta in surface expression of the TCR/CD3 complex and in activation for killing analyzed with a CD3 delta-negative cytotoxic T lymphocyte variant. J. Immunol., 148, 657-664
  6. Charlemagne, J., J.S. Fellah, A. De Guerra, F. Kerfourn and S. Partula. 1998. T-cell receptors in ectother-mic vertebrates. Immunol. Rev., 166, 87-102 https://doi.org/10.1111/j.1600-065X.1998.tb01255.x
  7. Clevers, H., B. Alarcon, T Wileman and C. Terhorst. 1988a. The T cell receptor/CD3 complex: a dyna-mic protein ensemble. Annu. Rev. Immunol., 6, 629-662 https://doi.org/10.1146/annurev.iy.06.040188.003213
  8. Clevers, H.C., S. Dunlap, T.E. Wileman and C. Terhorst. 1988b. Human CD3-$\varepsilon$gene contains miniexons and is transcribed from a non-TATA promoter. Proc. Natl. Acad. Sci. USA, 85, 8156-8160
  9. Dzialo, R.C. and M.D. Cooper. 1997. An amphibian CD3 homologue of the mammalian CD3 gamma and delta genes. Eur. J. Immunol., 27, 1640-1647 https://doi.org/10.1002/eji.1830270708
  10. Geisler, C. 1992. Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. J. Immunol., 148, 2437-2445
  11. Gobel, T.W. and M. Fluri. 1997. Identification and analysis of the chicken CD3 $\varepsilon$ gene. Eur. J. Immunol., 27, 194-198 https://doi.org/10.1002/eji.1830270129
  12. Gobel, T.W. and J.P. Dangy. 2000. Evidence for a stepwise evolution of the CD3 family. J. Immunol., 164, 879-883 https://doi.org/10.4049/jimmunol.164.2.879
  13. Hirono, I., B.H. Nam, T. Kurobe and T. Aoki. 2000. Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralichthys olivaceus. J. Immunol., 165, 4423 -4427 https://doi.org/10.4049/jimmunol.165.8.4423
  14. Huppa, J.B. and H.L. Ploegh. 1997. In vitro translation and assembly of a complete T cell receptor-CD3 complex. J. Exp. Med., 186, 393-403 https://doi.org/10.1084/jem.186.3.393
  15. Katagiri, T., S. Asakawa, I. Hirono, T. Aoki and N. Shimizu. 2000. Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus. Mar. Biotechnol., 2, 571-576 https://doi.org/10.1007/s101260000041
  16. Klausner, R.D., J. Lippincott-Schwartz and J.S. Bonifacino. 1990. The T cell antigen receptor: insights into organelle biology. Ann. Rev. Cell Biol., 6, 403-431 https://doi.org/10.1146/annurev.cb.06.110190.002155
  17. Krangel, M.S. 1987. Endocytosis and recycling of the T3- T cell receptor complex. The role of T3 phosphorylation. J. Exp. Med., 165, 1141-1159 https://doi.org/10.1084/jem.165.4.1141
  18. Krangel, M.S., B.E. Bierer, P. Devlin, M. Clabby, J.L. Strominger, J, McLean and Brenner M.B. 1987. T3 glycoprotein is functional although structurally distinct on human T-cell receptor gamma T lymphocytes. Proc. Natl. Acad. Sci. USA, 84, 3817-3821
  19. Krissansen, G.W., M.J. Owen, P.J. Fink and M.J. Crumpton. 1987. Molecular cloning of the cDNA encoding the T3 gamma subunit of the mouse T3/T cell antigen receptor complex. J. Immunol., 138, 3513-3518
  20. Mallabiabarrena, A., M. Fresno and B. Alarcon. 1992. An endoplasmic reticulum retention signal in the CD3$\varepsilon$ chain of the T-cell receptor. Nature, 357, 593-596 https://doi.org/10.1038/357593a0
  21. Manolios, N., F. Letourneur, J.S. Bonifacino and R.D. Klausner. 1991. Pairwise, cooperative and inhibitory interactions describe the assembly and probable structure of the T-cell antigen receptor. EMBO J., 10, 1643-1651
  22. Nam, B.H., I. Hirono and T. Aoki. 2003. Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A/PMA. Fish Shellfish Immunol., 14, 467-476 https://doi.org/10.1006/fsim.2002.0448
  23. Niedergang, F., A. Dautry-Varsat and A. Alcover. 1997. Peptide antigen or superantigen-induced downergulation of TCRs involves both stimulated and unstimulated receptors. J. Immunol., 159, 1703-1710
  24. Park C.I., I. Hirono, J. Enomoto, Nam B.H., T. Aoki. 2001. Cloning of Japanese flounder Paralichthys olivaceus CD3 cDNA and gene, and analysis of its expression. Immunogenetics, 53, 130-135 https://doi.org/10.1007/s002510100311
  25. Pasquier, L., M. Courtet and J. Robert. 1995. A Xenopus lymphoid tumor cell line with complete Ig genes rearrangements and T-cell characteristics. Mol. Immunol., 32, 583-593 https://doi.org/10.1016/0161-5890(95)00002-V
  26. Reth, M. 1989. Antigen receptor tail clue. Nature, 338, 383-344
  27. Rubin, B.J. Arnaud, S. Caspar-Bauguil, F. Conte and Huchenq 1994. A. Biological function of the extracellular domain of the T-cell receptor constant region. Scand. J. Immunol., 39, 517-525 https://doi.org/10.1111/j.1365-3083.1994.tb03408.x
  28. Sussman, J.J., J.S. Bonifacino, J. Lippincott-Schwartz, A.M. Weissman, T. Saito, R.D. Klausner and J.D. Ashwell. 1988. Failure to synthesize the T cell CD3- zeta chain: structure and function of a partial T cell receptor complex. Cell, 52, 85-95 https://doi.org/10.1016/0092-8674(88)90533-8
  29. Wange, R.L. and L.E. Samelson. 1996. Complex complexes: signaling at the TCR. Immunity, 5, 197-205 https://doi.org/10.1016/S1074-7613(00)80315-5