Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Antioxidative Effect of Plant Food Mixtures in Rat Fed on High Fat-High Cholesterol Diet

고지방고콜레스테롤식이를 섭취한 흰쥐에서 식물성식품의 혼합물 첨가로 인한 체내 항산화능 증가 효과

  • Kwak, Chung-Shil (Aging and Physical Culture Research Institute, Medical college of Seoul National University) ;
  • Kim, Mee-Yeon (Department of Food and Nutrition, Ewha Woman's University) ;
  • Lee, Mee-Sook (Department of Food and Nutrition, Hannam University)
  • 곽충실 (서울대학교 의학연구원 체력과학노화연구소) ;
  • 김미연 (이화여자대학교 식품영양학과) ;
  • 이미숙 (한남대학교 식품영양학과)
  • Published : 2005.06.01
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Abstract

The previous extensive in vitro studies on the antioxidative activities of a number of Korean grains, vegetables, seaweeds and mushrooms, and the various combinations of these food source exhibited a wide range of antioxidative activities, and four food mixtures composed of 5 kinds of foods (5A, 5B, 5C and 5D) were designed from 16 selective foods showing. high antioxidant effect, in vitro, to find the good combinations for the meal planning. Mixture 5B or 5C contained very high levels of total flavonoid and polyphenol, and ethanol extract from 5A, 5B or 5C showed very strong inhibitory effects against in vitro $Fe^{2+}-induced$ lipid peroxidation and ethanol extract from 5B or 5C showed remarkable DPPH radical scavenging effect and lipid peroxide-protein conjugation inhibition effect. And in vivo study was also carried out with two mixtures (5B, 5C). Powders (P5B, P5C) or ethanol extracts (E5B, E5C) of these mixtures were supplemented to Sprague-Dawley rats fed on high fat $(15\%)-high$ cholesterol $(1\%)$ semipurified diet for 5 weeks. The total antioxidant power in serum was significantly higher in P5B, P5C, E5B and E5C groups than in high fat control group, and $ascorbate-Fe^{2+}-induced$ TBARS was significantly lowered by E5B supplementation in rat liver. In liver tissue, Cu, Zn-SOD activity was significantly higher in P5B and E5B groups than in high fat control group, while catalase or GSH-peroxidase (GPx) activity was not changed by any supplementations. In kidney, Cu, Zn­SOD activity was significantly higher in P5B group than in high fat control group, while GPx activity was not changed by any supplementations. Taken together, mixture 5B and 5C showed very strong antioxidative effects both in vitro and in vivo. Therefore, the ingredient Korean foods of 5B and 5C could be recommended to take a lot together for prevention from age-related chronic diseases.

Keywords

References

  1. Ceruti PA. Prooxidant states and tumor promotion. Science 227: 375-381, 1985 https://doi.org/10.1126/science.2981433
  2. Sohal RS, Sohal BH, Brunk UT. Relationship between antioxidant defenses and longevity in different mammalian species. Mech Aging Dev 53: 217-227. 1990 https://doi.org/10.1016/0047-6374(90)90040-M
  3. Tappel AL. Lipid peroxidation damage to cell components. Fed Proc 32: 1870-1974, 1973
  4. Block G, Patterson B, Subar A. Fruit, vegetables and cancer prevention: A review of the epidemiological evidence. Nutr Cancer 18: 1-29, 1992
  5. Parfitt VJ, Rubba P, Bolton C, Marotta G, Hartog M, Mancini M. A comparison of antioxidant status and free radical peroxidation of plasma lipoproteins in healthy young persons from Naples and Bristol. Eur Heart J 15: 871-876, 1994
  6. Visioli F, Bogani P, Grande S, Galli C. Mediterranean food and health: Building human evidence. J Physiol Pharmacol 56 (s1) : 37-49,2005
  7. Tucker KL, Hallfrisch J, Qiao N, Muller D, Andres R, Fleg JL. The combination of high fruit and vegetable and low saturated fat intakes is more protective against mortality in aging men than is either alone: The Baltimore longitudinal study of aging. J Nutr 135: 556-561,2005
  8. Cutler RG. Antioxidants and aging. Am J Clin Nutr 53 (IsuppI) : 373s-379s, 1991
  9. Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, Remberg SF, Wold AB, Haffner K, Baugerod, Anderson LF, Moskang JO, Jacobs DR, Blomhoff RB. A systematic screeing of total antioxidants in dietary plants.J Nutr 132: 461-471,2002
  10. Ninfali P, Mea G, Giorgini S, Rocchi M, Bacchiocca M. Antioxidant capacity of vegetables, spices and dressings relevant to nutrition. Br J Nutr 93 (2) : 257-266,2005 https://doi.org/10.1079/BJN20041327
  11. Jelodar GA, Kaleki M, Motadayen MR, Sims S. Effect of fenugreek, onion and garlic on blood glucoses and histopathology of pancreas of alloxan-induced diabetic rats. Indian J Med Sci 59 (2) : 64-69, 2005 https://doi.org/10.4103/0019-5359.13905
  12. Suzuki F, Kobayashi M, Komatsu Y, Kato A, Pollard RB. Keishika- kei-to, a traditional Chinese herbal medicine, inhibits pulmonary metastasis of B16 melanoma. Anticancer Res 17: 873-878, 1997
  13. Uesugi T, Fukui Y, Yamori Y. Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum lipids in postmenopausal Japanese women: a four-week study. J Am Coll Nutr 21 (2) : 97-102,2002
  14. Rifici VA, Schneider SH, Khachadurian AK. Lipoprotein oxidation mediated by 1774 murine macrophage is inhibited by individual red wine polyphenols but not by ethanol. J Nutr 132: 2532-2537,2002
  15. Miller NJ, Rice-Evans CA. Antioxidant activity of resveratrol in red wine. Clin Chern 41: 1789, 1995
  16. Surb YJ, Hurb YJ, Kang JY, Lee E, Kong G, Lee SJ. Resveratrol, an antioxidant present in red wine, induces apopotosis in human promyelocyte leukemia (HL-60) cells. Cancer Letters 140: 110, 1999
  17. Kogure K, Goto S, Nishimura M, Yasumoto M, Abe K, Ohiwa C, Sassa H, Kusurni T, Terada H. Mechanism of potent antiperoxidative effect of capsaicin. Biochirn Biophys Acta 1573 (I): 84-92,2002
  18. Lee JS, Chang JS, Lee JY, Kim JA. Capsaicin-induced apoptosis and reduced release of reactive oxygen species in MBT-2 murine bladder tumor cells. Arch Pharm Res 27 (II): 1147-1153,2004 https://doi.org/10.1007/BF02975121
  19. Lin YL, Cheng CY, Lin YP, Lau YN, Juan IM, Lin JK. Hypolipidemic effect of green tea leaves through induction of antioxidant and phase II enzymes including superoxide dismutase, catalase and glutathione S-transferase. J Agric Food Chern 46: 1893-1899, 1998 https://doi.org/10.1021/jf970963q
  20. Rao CV, Rivenson A, Simi B, Reddy BS. Chemoprevention of colon carcinogenesis by dietary curcurnin, a naturally occuring plant phenolic compound. Cancer Res 55: 259-266, 1995
  21. Katiyar SK, Agarwal R, Mukhtar H. Inhibition of tumor promotion in SENCAR mouse skin by ethanol extract of Zingober officinale rhizome. Cancer Res 56: 1023-1030,1996
  22. Report on 2001 National Health and Nutrition Survey. Ministry of Health and Welfare, 2002
  23. Im KJ, Lee SK, Park OK, Rhee MS, Lee JK. Inhibitory effects of garlic extracts on the nitrosation. J Korean Soc Agric Chern Biotechal 43 (2): 110-115, 2000
  24. Jo HJ, Choi MJ. Effect of 1% garlic powder on serum and liver lipid and plasma amino acid concentration in rats fed cholesterol diet.J Korean Soc Food Sci Nutr 31 (I): 98-103,2002
  25. Shoo HJ. The antibacterial action of garlic, onion, ginger and red pepper juice. J Korean Soc Food Sci Nutr 28(1): 94-99, 1999
  26. Cho SY, Han YB, Shin KU. Screening for antioxidant activity of edible plants.J Korean Soc Food Sci Nutr 30(1): 133-137,2001
  27. Sohn HE, Lee JY, Kim DC, Hwang WI. Enhancement of anticancer activity by combination of garlic (Allium Sativum) extract and Vitamin C. J Korean Soc Food Sci Nutr 30(2): 372-376, 2001
  28. Park JC, Ha JO, Park KY. Antimutagenic effect of flavonoids isolated from Oenanthe javanica. J Korean Soc Food Sci Nutr 25: 588-592, 1996
  29. Kang JO. Antioxidative activity of Mugwort extract on human low density lipoprotein. Kor J Soc Food Sci 16 (6): 623-628, 2000
  30. Kwak HJ, Kwon YJ, Jeong PH, Kwon JH, Kim HK. Physiological activity and antioxidative effect of methanol extract from Onion (Allium cepa L). Korean J Nutr 29 (2): 349-355,2000
  31. Bae JT, Chang JS, Lee KR. Effect of Saecodon asparatus extract on expression of cell cycle-associated proteins in HepG2 cells. J Korean Soc Food Sci Nutr 31 (2) : 329-332,2002
  32. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J Korean Soc Food Sci Nutr 32 (2) : 217-222,2003
  33. Jung SW, Kim MK. Effect of dried powdered of Chamomile, Sage and Green tea on antioxidative capacity in 15-month-old rats. Korean J Nutr 36(7): 699-710,2003
  34. Kim ES, Kim MK. Effect of dried leaf powders and ethanol extracts of Persimon, Green tea and Pine needle on lipid metabolism and antioxidative capacity in rats. Korean J Nutr 32(4): 337-352, 1999
  35. Park KY, Cho EJ, Rhee SH. Increased antimutagenic and anticancer activities of Chines cabbage Kimchi by changing and levels of sub-ingredient. J Korean Soc Food Sci Nutr 27 (4) : 625-632, 1998
  36. Kim HJ, Lee IS, Lee KR. Antimutagenic and anticancer effects of Ramaria botrytis Rick extracts. J Korean Soc Food Sci Nutr 28 (6): 1321-1325,1999
  37. Kim YD, Yang WM. Research on component of wild plant. Korean J Food Sci Technol15 (4): 10-16,1991
  38. Lee MS, Lim SJ, Kim SA, Woo MK, Kwak CS, Park SC. Antimutagenic and antioxidative effects of ethanol extracts from Garlic, Ginger, Green onion and Red pepper. Kor J Gerontology 13 (1 ) : 8-16,2003
  39. Oh SI, Lee MS. Screening for antioxidative and antimutagenic capacities in 7 common vegetables taken by Korean. J Korean Soc Food Sci Nutr 32 (8): 1344-1350,2003
  40. Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS. Antioxidative and antimutagenic effects of Korean Buckwheat, Sorghum, Millet and Job's tears. J Korean Soc Food Sci Nutr 33(6): 921-929, 2004 https://doi.org/10.3746/jkfn.2004.33.6.921
  41. Kim SA, Kim J, Kwak CS, Lee MS. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J Korean Soc Food Sci Nutr 34(4): 451-458,2005 https://doi.org/10.3746/jkfn.2005.34.4.451
  42. Antonella S, Mario S, Maria L, Daniela M, Francesco B, and Francesco C. F1avonoids as antioxidant agents: Importance of their interaction with biomembranes. Free Rad Bio Med 19(4): 481-486, 1995 https://doi.org/10.1016/0891-5849(94)00240-K
  43. Gotteried H and Dunkley WL. Ascorbic acid and copper in linoleate oxidation: Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test. J Lipid Res 10: 555-560, 1969
  44. Blois MS. Antioxidant detennination by the use of a stable radical. Nature 26: 1199-1200,1958
  45. Park YR. Effect of polyamine on modification of biomodics by aldehydes. Ph. D thesis. Seoul National University, 2000
  46. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72: 248-254, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  47. Gomez-Sanchez A, Hermonsin I, Mayo I. Cleavage and oligomerization of malondialdehyde under physiological conditions. Tetrahedron Letters 28: 4077-4080, 1990
  48. Lee YC, Hwang KH, Han DH, Kim SD. Compositions of Opumtia fiscus-medica. Korean J Food Sci Technol 29 (5) : 847-853, 1997
  49. Chae SK, Kang GS, Ma SJ, Bang KW, Oh MW, Oh SH. Standard Food Analysis. Jigu-moonwha Sa, pp. 381-382, 2002
  50. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158,1965
  51. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of 'Antioxidant power': The FRAP Assay. Anal Biochem 239: 70-76, 1996 https://doi.org/10.1006/abio.1996.0292
  52. Miller DM, Aust SD. Studies of ascorbate-dependent, iron-catalyzed lipid peroxidation. Arch Biochem Biophys 271: 113-120, 1989 https://doi.org/10.1016/0003-9861(89)90261-0
  53. Lowry OH, Rosebrough NJ, Farr AL and Rndall RI. Protein measurement with the Folin phenol reagent. J Bioi Chem 193: 265-275, 1951
  54. Aebi. Catalase in vitro. In: Methods in Enzymology 105: 121-126, 1984 https://doi.org/10.1016/S0076-6879(84)05016-3
  55. Tappel AL. Glutahione peroxidase and hydroperoxides. In methods in Enzymology 52: 506-513, 1978 https://doi.org/10.1016/S0076-6879(78)52055-7
  56. Habig WH, Pabst MI and Jakoby WB. Glutathion S-transferase. J Bioi Chem 249: 7130-7139, 1974
  57. Misra HP and Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase. J Bioi Chem 247: 3170-3175, 1972
  58. Stirpe F, Della Corte E. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Bioi Chem 244(14): 3855-3863, 1969
  59. Otamari T and Sjodahl R. Increased lipid peroxidation in malignant tissues of patients with colorectal cancer. Cancer 64: 422-425, 1989 https://doi.org/10.1002/1097-0142(19890715)64:2<422::AID-CNCR2820640214>3.0.CO;2-2
  60. Gutteridge JM. Lipid peroxidation and antioxidants as biomarker of tissue damage. Clin Chem 41 (pt 2): 1819-1826,1995
  61. Mattson MP. Mother's legacy: mitochondrial DNA mutations and Alzheimer's disease. Trends Neurosci 20: 373-280, 1997 https://doi.org/10.1016/S0166-2236(97)01114-4
  62. Kim SK, Lee HI, Kim MK. Effect of water and ethanol extracts of persimmon leaf and green tea different conditions on lipid metabolism and antioxidative capacity in 12-month-old rats. Korean J Nutr 34 (5) : 499-512, 2001
  63. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Miller A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84: 407-412, 1993
  64. Frei B, Stocker R, Ames BN. Antioxidant defenses and lipid peroxidation in human plasma. Acad Aci USA 85: 9748-9752, 1988 https://doi.org/10.1073/pnas.85.24.9748
  65. Terada LS, Leff A, Repine JE. Measurement of xanthine oxidase in biological tissues. In: Methods in Enzymology. vol 186, Academic Press, pp.651-652, 1990
  66. Hub K, Shin US, Lee SI. Effect of heat-treated ceruloplasmin on the hepatic xanthine oxidase activity and type conversion. Arch Pharm Rec 18 (1): 56-59, 1995 https://doi.org/10.1007/BF02976510
  67. Hayashi T, Sawa K, Kawasaki M, Arisawa M, Shimisu M, Morita M. Inhibition of cow's milk xanthine oxidase by flavonoids. J Natural Products 51: 345-348, 1988 https://doi.org/10.1021/np50056a030
  68. Ra KS, Chung SH, Sub HJ, Son JY, Lee HK. Inhibitor of xanthine oxidase from onion skin. Korean J Food Sci Technol 30 (3) : 697-701,1998
  69. RLee SJ, Choi JH. Effects of green tea catechins on the superoxide dismutase, glutathione peroxidase and xanthine oxidase activities of kidney in diabetic rats. Korean J Nutr 34(7): 734-740,2001
  70. Bompart GJ, Prevot DS, Bascands JL. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 23 (6) : 501-504, 1990 https://doi.org/10.1016/0009-9120(90)80039-L
  71. Mo JQ, Hom DG, Anderson JK. Decreases in protective enzymes correlates with increased oxidative damage in the aging mouse brain. Mech Aging Dev 81: 73-80, 1995 https://doi.org/10.1016/0047-6374(95)01586-O
  72. Azhar S, Cao L, Reaven E. Alteration of adrenal antioxidant defence system during aging in rats. J Clin Invest 96: 1414-1420, 1995 https://doi.org/10.1172/JCI118177
  73. Hyun KW, Kim JH, Song KJ, Lee JB, Jang JH, Kim YS, Lee JS. Physiological functionality in Geumsan perilla leaves from greenhouse and field cultivation. Korean J Food Sci Technol 35 (5): 975-979,2003
  74. Lee KI, Rhee SK, Park KY, Kim JO. Antimutagenic compounds identified from perilla leaf. Korean J Soc Food Nutr 21: 302-307, 1992
  75. Lee E. Effects of powdered pine needle on serum and liver lipid composition and antioxidative capacity in rats fed high oxidized fat. J Kor Soc Food Sci Nutr 32 (6) : 926-930,2003
  76. Park CS, Kwon CJ, Choi MA, Park KS, Choi KH. Scavenging activities of mugwort and pine needle extracts. Kor J Food Preserv 9 (2) : 248-252, 2002
  77. Kim HY, Lee JH, Choi JH, Kim DI, Park SH, Baik SJ, Cho WG. Effects of butanol fraction of pine needle on oxygen radical and their scavenging enzymes in liver of SD rats. Kor J Nutr 35 (3) : 292-295, 2002
  78. Kang YR, Park YK, Ha TY, Moon KD. Effects of Pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Socfood Nutr 25 (3): 367-373, 1996