Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Cloned Placenta of Korean Native Calves Died Suddenly at Two Months after Birth Displays Differential Protein Expression

  • Kim Hong Rye (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Kang Jae Ku (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Lee Hye Ran (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Yoon Jong Taek (Genetic Engineering Institute, Hankyong National University) ;
  • Seong Hwan Hoo (National Livestock Research Institute, RDA) ;
  • Jung Jin Kwan (National Livestock Research Institute, RDA) ;
  • Park Chang Sik (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Jin Dong Il (Research Center for Transgenic Cloned Pigs. Chungnam National University)
  • Published : 2005.06.01
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Abstract

Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, $H^+-transporting$ ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.

Keywords

References

  1. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW, Echelard Y (1999): Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17:456-461 https://doi.org/10.1038/8632
  2. Cezar GG, Bartolomei MS, Forsberg EJ, First NL, Bishop MD, Eilertsen KJ (2003): Nome-wide Epigenetic alterations in cloned bovine fetuses. Biol Reprod 68:1009-14 https://doi.org/10.1095/biolreprod.102.010181
  3. Chavatte-Palmer P, Heyman Y, Richard C, Monget P, LeBourhis D, Kann G, Chilliard Y, Vignon X, Renard JP (2002): Clinical, hormonal, and hematologic characteristics of bovine calves derived from nuclei from somatic cells. Biol Reprod 66: 1596-1603 https://doi.org/10.1095/biolreprod66.6.1596
  4. Chung YG, Ratnam S, Chaillet JR, Latham KE (2003): Abnormal regulation of DNA methyltransferase expression in cloned mouse embryos. Biol Reprod 69: 146-53 https://doi.org/10.1095/biolreprod.102.014076
  5. Cibelli JB, Campbell KH, Seidel GE, West MD, Lanza RP (2002): The health profile of cloned animals. Nat Biotechnol 20:13-14 https://doi.org/10.1038/nbt0102-13
  6. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C (1998): Ponce de Leon FA, Robl JM. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280: 1256-1258 https://doi.org/10.1126/science.280.5367.1256
  7. Dyer O (2002): Dolly's arthritis dents faith in cloning. BMJ 324:67 https://doi.org/10.1136/bmj.324.7329.67/a
  8. Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W (2000): The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21:1037-1053 https://doi.org/10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V
  9. Han YM, Kang YK, Koo DB, Lee KK (2003): Nuclear reprogramming of cloned embryos produced in vitro. Theriogenology 59:33-44 https://doi.org/10.1016/S0093-691X(02)01271-2
  10. Hill JR, Burghardt RC, Jones K, Long CR, Looney CR, Shin T, Spencer TE, ThompsonJA, Winger QA, Westhusin ME (2000): Evidence for placental abnormalityas the major cause of mortality in first-trimester somatic cell cloned bovine fetuses. Biol Reprod 63:1787-1794 https://doi.org/10.1095/biolreprod63.6.1787
  11. Hill JR, Edwards JF, Sawyer N, Blackwell C, Cibelli JB (2001): Placental anomalies in a viable cloned calf. Cloning 3:83-88 https://doi.org/10.1089/15204550152475581
  12. Hill JR, Roussel AJ, Cibelli JB, Edwards JF, Hooper NL, Miller MW, Thompson JA, Looney CR, Westhusin ME, Robl JM, Stice S (1999): Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies). Theriogenology 51:1451-1465 https://doi.org/10.1016/S0093-691X(99)00089-8
  13. Hill JR, Schlafer DH, Fisher PJ, Davies CJ (2002): Abnormal expression of trophoblast major histocompatibility complex class I antigens in cloned bovine pregnancies is associated with a pronounced endometrial lymphocytic response. Biol Reprod 67:55-63 https://doi.org/10.1095/biolreprod67.1.55
  14. Hill JR, Winger QA, Long CR, Looney CR, Thompson JA, Westhusin ME (2000): Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells. Biol Reprod 62:1135-1140 https://doi.org/10.1095/biolreprod62.5.1135
  15. Jensen ON, Wilm M, Shevchenko A, Mann M (1999): Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels. Methods Mol Biol 112:513-530
  16. Joubert-Caron R, Le Car JP, Montandon F, Poirier F, Pontet M, Imam N, Feuillard J, Bladier D, Rossier J, Caron M (2000): Protein analysis by mass spectrometry and sequence database searching: a proteomic approach to identify human lymphoblastoid cell line proteins. Electrophoresis 21:2566-2575 https://doi.org/10.1002/1522-2683(20000701)21:12<2566::AID-ELPS2566>3.0.CO;2-F
  17. McCreath KJ, Howcroft J, Campbell KH, Colman A, SchniekeAE, Kind AJ (2000): Production of genetargeted sheep by nuclear transfer from cultured somatic cells. Nature 405:1066-1069 https://doi.org/10.1038/35016604
  18. McGraw S, Robert C, Massicotte L, Sirard MA (2003): Quantification of histone acetyltransferase and histone deacetylase transcripts during early bovine embryo development. Biol Reprod 68:383-9 https://doi.org/10.1095/biolreprod.102.005991
  19. Ono Y, Shimozawa N, Ito M, Kono T (2001): Cloned mice from fetal fibroblast cells arrested at metaphase by a serial nuclear transfer. Biol Reprod 64:44-50 https://doi.org/10.1095/biolreprod64.1.44
  20. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000): Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90 https://doi.org/10.1038/35024082
  21. Reik W, Santos F, Dean W (2003): Mammalian epigenomics: reprogramming thegenome for development and therapy. Theriogenology 59:21-32 https://doi.org/10.1016/S0093-691X(02)01269-4
  22. Tamashiro KL, Wakayama T, Akutsu H, Yamazaki Y, Lachey JL, Wortman MD, Seeley RJ, D'Alessio DA, Woods SC, Yanagimachi R, Sakai RR (2002) Cloned mice have an obese phenotype not transmitted to their offspring. Nat Med 8:262-267 https://doi.org/10.1038/nm0302-262
  23. Tanaka S, Oda M, Toyoshima Y, Wakayama T, Tanaka M, Yoshida N, Hattori N, Ohgane J, Yanagimachi R, Shiota K (2001): Placentomegaly in cloned mouse conception caused by expansion of the spongiotrophoblast layer. Biol Reprod 65:1813-1821 https://doi.org/10.1095/biolreprod65.6.1813
  24. Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374 https://doi.org/10.1038/28615
  25. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813 https://doi.org/10.1038/385810a0
  26. Young LE, Sinclair KD, Wilmut I (1998): Large offspring syndrome in cattle and sheep. Rev Reprod 3:155-163 https://doi.org/10.1530/ror.0.0030155