Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지

Effect of Colostral Whey Fraction on the Proliferation of EL-4 Cell

초유 유청분획의 EL-4 세포 증식 효과

  • Ha Woel-Kyu (Food Research & Development Lab., Pasteur Milk Co., Ltd.) ;
  • Won Do-Hee (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Yang Hee-Jin (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Hwang Kyung-A (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Lee Soo-Won (Department of Food Biotechnology, Sungkyunkwan University)
  • 하월규 (파스퇴르유업㈜ 식품연구소) ;
  • 원도희 (성균관대학교 식품생명공학과) ;
  • 양희진 (성균관대학교 식품생명공학과) ;
  • 황경아 (성균관대학교 식품생명공학과) ;
  • 이수원 (성균관대학교 식품생명공학과)
  • Published : 2005.06.01
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Abstract

To investigate the effect of Holstein colostrum peptide fraction on proliferation of immune cell, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. EL-4 cell (murine T lymphoma cell) was used to evaluate immune enhancing effect of each fraction from Holstein colostrum. Fraction n showed the highest proliferative effect of the colostrum whey fractions on EL-4 cell at 1mg/mL compared with whole whey and other fractions and this proliferative activity was shown in dose dependent manner. Fraction n showed the highest proliferative effect on PMA (Phorbol 12-myristate 13-acetate) stimulated EL-4 cell. Heated Fraction n showed similar effect to native one on proliferation of both EL-4 cell and PMA stimulated EL-4 cell.

Keywords

References

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