Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibition Effects of Natural Products on Osteoclast Differentiation

천연물 추출물의 파골세포 분화억제 효과 검색

  • Lee, Hyo-Jung (Department of Food Science & Technology, Keimyung University) ;
  • Yu, Mi-Hee (Department of Food Science & Technology, Keimyung University) ;
  • Lee, Syng-Ook (Department of Food Science & Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources center, Keimyung University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources center, Keimyung University)
  • 이효정 (계명대학교 식품가공학과) ;
  • 유미희 (계명대학교 식품가공학과) ;
  • 이승욱 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터)
  • Published : 2005.12.31
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Abstract

In bone remodeling imbalances that are caused by increased bone resorption over bone formation lead to adult skeletal diseases. Thus, we have screened various natural products for their ability to regulate the differentiation of osteoclasts to propose candidates for the prevention or treatment of osteoporosis. Scutellaria baicalensis Georgi and Zizyphus Jujuba Miller var. extracts of 140 natural products inhibited the differentiation of RAW264.7 cells into osteoclast, as showed by the reduced number of tartrate resistant acid phosphatase(TRAP)-positive multinucleated cells and decreased TRAP activity.

여러 천연물 추출물 140종의 파골세포 분화처제효과 검색은 파골세포의 마커 enzyme인 TRAP활성으로 확인하였다. 그 결과 파고지$^{3}$, 지각$^{13}$, 금령자$^{6}$, 시호$^{9}$, 황연$^{69}$, 배양인삼$^{98}$, 구맥$^{26}$, 등 대부분의 추출물이 $100{\mu}g/mL$ 농도 이상에서는 높은 세포 독성을 보여서 파골분화 억제 정도를 확인 할 수 없었고 세포독성이 없는 농도를 선정하여 파골세포 분화억제 정도를 재검색한 결과는 천연물 중 파고지$^{3}$, 호두$^{21}$, 용안육씨$^{77}$, 황금$^{78}$, 정공등$^{37}$ 메탄올 추출물과 생대추과육$^{128}$, 콩나물$^{70}$ 열수 추출물은 비교적 낮은 농도에서도 30% 이하의 TRAP 활성을 보여 파골분화 억제 효과가 우수하게 나타났다. 이 중 전통 한약재인 황금$^{78}$ 메탄올 추출물과 과일류인 생대추과육$^{128}$ 열수 추출물이 $10{\mu}g/mL$의 낮은 농도에서도 가장 좋은 파골분화 억제 효과가 나타났다.

Keywords

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