Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Genotypic and Phenotypic Characteristics of Staphylococcus aureus Isolates from Lettuces and Raw Milk

상추와 원유에서 분리한 황색 포도상구균의 유전형 및 표현형 특징

  • Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University) ;
  • Cho, Joon-Il (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Kim, Cheol-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Song, Eun-Seop (Department of Obstetrics and Gynecology, College of Medicine, Inha University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea research Institute of Bioscience and Biotechnology) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
  • 정혜진 (중앙대학교 식품공학과) ;
  • 조준일 (중앙대학교 식품공학과) ;
  • 박성희 (중앙대학교 식품공학과) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 이규호 (한국외국어대학교 환경학과) ;
  • 김철호 (동국대학교 한의과대학 생화학교실) ;
  • 송은섭 (인하대학교 의과대학 산부인과학교실) ;
  • 정덕화 (경상대학교 식품공학과) ;
  • 김민곤 (생명공학연구원) ;
  • 김광엽 (충북대학교 식품공학과) ;
  • 김근성 (중앙대학교 식품공학과)
  • Published : 2005.02.28
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Abstract

To characterize genotypic and phenotypic traits of Staphylococcus aureus isolates (n = 86) from lettuces and raw milk, major virulence-associated genes and antibiotic susceptibility were detected using PCR-based methods and disk diffusion method, respectively. All isolates possessed coagulase gene and showed five polymorphism types [500 bp (2.4%), 580 bp (17.4%), 660 bp (61.6%), 740 bp (17.4%), and 820 bp (1.2%)] due to variable numbers of tandem repeats present within the gene. Two or three different loci of hemolysin gene family were dominant in isolates, 47 of which (55%) possessed combination of hla/hld/hlg-2 genes as the most prevalent types. Among enterotoxin-encoding genes, sea was detected from 32 isolates (37%), sed from 1 isolate (1%), and sea and sed genes were co-detected from 4 isolates (5%), whereas seb, sec, and tsst-1 genes were not detected. All isolates were susceptible to ciprofloxacin, trimethoprim/sulfamethoxazole, oxacillin, and vancomycin, 85 isolates (99%) to penicillin G, 54 isolates (63%) to chloramphenicol, 51 isolates (59%) to erythromycin, and 7 isolates (8%) to clindamycin. Among resistant isolates, seven displayed multiantibiotic-resistance against two different antibiotics.

S. aureus는 자연계에 널리 상재해 있으며 우리나라의 경우 Salmonella spp.에 의한 식중독 다음으로 많이 발생하는 식중독의 원인균으로 알려져 있다. 따라서 본 실험에서는 상추와 원유로부터 분리한 S. aureus(n=86)의 표현형 및 유전형 특징을 파악할 목적으로 coagulase, hemolysin, enterotoxin 및 toxic shock syndrome toxin I 등에 대한 유전자를 대상으로 PCR 방생제 감수성 시험을 수행하여 그들 분리균주들의 항생제 내성정도를 결정하였다. 상추와 원유로부터 분리한 S. aureus 균주는 모두 coagulase 유전자를 보유하고 있었고, 또한 PCR 산물의 amplicon size는 500bp(2.4%), 580bp(17.4%), 660bp(61.6%), 740bp(17.4%) 및 820bp(1.2%)로 다섯 종류가 검출되었다. Hemolysin 유전자의 경우는 원유로부터 분리한 4개의 hld 유전자 보유균주를 제외하고 모든 분리균주에서 multiple hemolysin gene을 보유하였으며, 그 중 47개 균주(54.7%)가 hla/hld/hlg2 유전자를 모두 소유한 균주로서 가장 많은 분포를 나타내었다. 한편, enterotoxin 유전자를 소유한 S. aureus 분리균주는 37 균주(43.0%)로서 그중 32균주(37.2%)에서 sea 유전자가 검출되었고, 1균주(1.1%)에서 sed 유전자, 그리고 4균주(4.6%)에서 sea와 sed 유전자가 모두 검출되었으나, seb, sec 혹은 tsst-1 유전자는 검출되자 않았다. 그리고 항생제 감수성 시험 결과에서는 모든 균주가 ciprofloxacin, oxacillin, vancomycin 그리고 trimethoprim/sulfamethoxazole에 대하여 감수성을 나타내었고 penicillin G(98.8%)는 높은 감수성을 보였으나 chloramphenicol(63%)과 erythromycin(59%)은 감수성이 낮았고, chloramphenicol, clindamycin, erythromycin 및 penicillin G에 대하여는 각각 4.7%, 82.6%, 14.0% 및 1.2%의 균주가 내성을 가지고 있었다. 또한 항생제 다제내성 양상을 살펴본 결과에 의하면 사용된 8종류의 항생물질들 중 2가지 항생물질에 대하여 내성을 나타낸 균주는 7개(8.1%)이고, 3종 이상의 항생물질에 대하여 다제 내성을 보이는 균주는 나타나지 않았다.

Keywords

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