한국식생활문화학회지 (Journal of the Korean Society of Food Culture)

  • 제20권5호
  • /
  • Pages.603-607
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  • 2005
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  • 1225-7060(pISSN)
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  • 2288-7148(eISSN)
한국식생활문화학회 (Korean Society of Food Culture)
권호 표지

페놀성 화합물의 항충치활성 및 Glucosyltransferase 억제효과

Anticariogenic Activity and Glucosyltransferase Inhibition of Phenolic Compounds

  • 김선재 (여수대학교 식품공학.영양학부) ;
  • 박인배 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 강성국 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 정동옥 (초당대학교 조리과학부) ;
  • 정순택 (목포대학교 식품공학과 및 식품산업기술연구센터)
  • Kim, Seon-Jae (Division of Food Technology and Nutrition, Yosu National University) ;
  • Park, In-Bae (Department of Food Engineering and Food Industrial Technology Research Center, Mokpo National University) ;
  • Kang, Seong-Gook (Department of Food Engineering and Food Industrial Technology Research Center, Mokpo National University) ;
  • Chung, Dong-Ok (Department of Culinary Art, Chodang University) ;
  • Jung, Soon-Teck (Department of Food Engineering and Food Industrial Technology Research Center, Mokpo National University)
  • 발행 : 2005.10.31
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초록

충치원인균인 Streptococcus mutans에 대한 페놀성 화합물(14종)의 항미생물활성은 hydroxybenzoic acid group에서는 syringic acid를 제외한 모든 화합물이 5와 10 mg/disc 함유량에서 $8.5{\sim}18mm$까지의 생육저해환을 나타내었다. 기타 phenolics 화합물에서는 catechol과 L-ascorbic acid가 저해능을 나타냈는데, 특히 catechol은 10 mg/disc 함유량은 $18.5{\sim}19.5mm$로 강한 항미생물활성을 나타냈다. Phenolic compounds의 최소저해농도는 S. mutans, M1 및 M2 균 모두에서 2,000 ppm의 농도에서 MIC 값을 나타내어 큰 차이를 보이지 않았으며, catechol만 1,000 ppm의 농도에서 높은 MIC 값을 나타내었다. 충치 관련 미생물인 S. mutans에 대해 항미생물활성이 인정된 7종의 페놀성 화합물을 대상으로 S. mutans가 생산하는 glucosyltransferase(GTase)의 활성저해능은 benzoic acid와 p-hydroxybenzoic acid 처리구에서는 100과 500 ppm 농도에서 약하게나마 GTase활성을 저해하는 경향을 나타냈으며, gallic acid 처리구는 10 ppm부터 활성을 나타내기 시작해서 100 ppm과 500 ppm에서 약 50%의 저해 활성을 나타냈다. 한편 catechol 처리구에서는 10 ppm에서 58.7%의 저해활성을 나타내었고, 50 ppm에서 60.7%, 100 ppm에서 88.4%, 그리고 500 ppm에서 89.6%의 높은 활성을 나타내어 catechol의 S. mutans에 대한 생육저해능 측정에서 높은 항미생물활성을 나타낸 결과와 동일한 경향을 나타냈다.

Fourteen phenolic compounds(benzoic acid, p-hydroxybenzoic acid, protocatechuic acid, vanillic acid, syringic acid, gallic acid, caffeic acid, ferulic acid, (+)-catechin, quercetin, rutin, catechol, chlorogenic acid and L-ascorbic acid) were examined for their effects on the anticarigenic activity. Among tested samples, catechol was significantly inhibited the S. mutans, exhibiting an clear zone 18.5-19.5mm by 10 mg/disc level. The minimal inhibition concentration(MIC) of the phenolic compounds for Streptococcus mutans, M1 and M2 strain were determined as 2,000 ppm, whereas catechol was 1,000 ppm. The activity of glucosyltransferase(GTase) was significantly inhibited by catechol, at 10 ppm(58.7%), 50 ppm(60.7%) and 100 ppm(88.4%) and 500 ppm(89.6%), respectively. Among them, catechol showed most significant anticariogenic activity as well as inhibition of GTase activity.

키워드

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