Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제31권5호
  • /
  • Pages.363-369
  • /
  • 2005
  • /
  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

MG-63 세포주에서 Vascular Endothelial Growth Factor (VEGF) mRNA 발현에 대한 Insulin-like Growth Factor I (IGF-I)의 효과에 대한 연구

THE EFFECTS OF INSULIN-LIKE GROWTH FACTOR I (IGF-I) ON EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) MRNA IN MG-63 OSTEOBLASTLIKE CELLS

  • 서제덕 (서울대학교 보라매병원 치과 구강악안면외과) ;
  • 명훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 강나라 (이화여자대학교 의과대학 구강악안면외과) ;
  • 정필훈 (서울대학교 치과대학 구강악안면외과학교실)
  • Suh, Je-Duck (Department of Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital) ;
  • Myung, Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry) ;
  • Kang, Nara (Department of Oral and Maxillofacial Surgery, Ewha Womans University, College of Medicine) ;
  • Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry)
  • 발행 : 2005.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea $(76.1\;{\mu}g/ml)$, actinomycin D $(2.5\;{\mu}g/ml)$, cycloheximide $(10\;{\mu}g/ml)$ were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time-and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.

키워드

참고문헌

  1. Miel C, Rochford JJ, Filippa N,Giorgetti-Peraldi S, Van Obberghen E: Insulin and insulin-like growth factor-I induce vascular endothelial growth factor mRNA expression via different signaling pathways. J Biol Chem 2000;275:21695-21702 https://doi.org/10.1074/jbc.M000805200
  2. Yeh LCC, Lee JC: Osteogenic Protein - 1 increases gene expression of vascular endothelial growth factor in primary cultures of fetal rat calvaria cells. Mol Cell Endocrinol 1999;153:113-124 https://doi.org/10.1016/S0303-7207(99)00076-3
  3. Trueta J: The role of the vessels in osteogenesis. J Bone Joint Surg 1963; 45B:402-418
  4. Decker B, Bartels H, Decker S: Relationships between endothelial cells, periocytes, and osteoblasts during bone formation in the sheep femur following implantation of tricalciumphosphate ceramic. Anat Rec 1995;242:310-320 https://doi.org/10.1002/ar.1092420304
  5. Deckers MM, Karperien M, Van der Bent C, Yamashita T, Papapoulos SE, Lowik CW: Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation. Endocrinol 2000; 141:1667-1674 https://doi.org/10.1210/en.141.5.1667
  6. Goad DL, Rubin J, Wang H, Tashjian AH Jr, Patterson C: Enhanced expression of vascular endothelial growth factor in human SaOS-2 osteoblast-like cells and murine osteoblasts induced by Insulin-like growth factor I. Endocrinol 1996;137:2262-2268 https://doi.org/10.1210/en.137.6.2262
  7. Brighton CT: Structure and function of the growth plate. Clin Orthop Rel Res 1978;136:22-32
  8. Brighton CT, Hunt RM: Early histological and ultrasrtuctural changes in medullary fracture callus. J Bone Joint Surg 1991;73-A:832-847
  9. Bermont L, Lamielle F, Fauconnet S, Esumi H, Weisz A, Adessi GL: Regulation of vascular endothelial growth factor expression by insulin-9 like growth factor-I in endometrial adenocarcinoma cells. Int J Cancer 2000;85:117-123 https://doi.org/10.1002/(SICI)1097-0215(20000101)85:1<117::AID-IJC21>3.0.CO;2-X
  10. Houck KA, Ferrara N, Winer J, Cachianes G, Li B, Leung DW: The vascular endothelial growth factor family: Identification of a fourth molecular species and characterization of alternative splicing of RNA. Mol Endocrinol 1991;5:1806-1814 https://doi.org/10.1210/mend-5-12-1806
  11. De Vries C, Escobedo JA, Ueno H, Houck K, Ferrara N, Williams LT: The fins-like tyrosine kinase, a receptor for vascular endothelial growth factor. Science 1992;255:989-991 https://doi.org/10.1126/science.1312256
  12. Quinn TP, Peters KG, De Vries C, Ferrara N, Williams LT: Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc Natl Acad Sci 1993;90:7533-7537
  13. Ferrara N, Davis-Smith T: The biology of vascular endothelial growth factor. Endocr Rev 1997;18:4-25 https://doi.org/10.1210/er.18.1.4
  14. Gerber HP, Vu TH, Ryan AM, Kowalski J, Werb Z, Ferrara N: VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat Med 1999;5:623-628 https://doi.org/10.1038/9467
  15. Hodgson DR: Free-ligand accelerated dissociation of insulin-like growth factor 1 (IGF-1) from the type I IGF receptor is reduced by insulin-like growth factor binding protein 3.Regulatory Peptides 2000;90:33-37 https://doi.org/10.1016/S0167-0115(00)00099-9
  16. Yeh LCC, Adamo ML, Kitten AM, Olson MS, Lee JC: Osteogenic Protein-1-mediated IGF gene expression in primary cultures of rat osteoblastic cells. Endocrinol 1996;137:1921-1931 https://doi.org/10.1210/en.137.5.1921
  17. Garcia-Ramirez M, Toran N, Andaluz P, Carrascosa A, Audi L: Vascular endothelial growth factor is expressed in human fetal growth cartilage. J Bone Miner Res Mar 2000;15:534-540 https://doi.org/10.1359/jbmr.2000.15.3.534
  18. Wang DS, Miura M, Demura H, Sato K: Anabolic effects of 1, 25- Dihydroxyvitamin D3 on Osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells. Endocrinol 1997;138:2953-2963 https://doi.org/10.1210/en.138.7.2953
  19. Zaldi M, Alam ASMT, Bax BE, Shanker VS, Bax CMR, Gill JS, Pazianas M, Huang CLH, Sahinoglu T, Moonga BS, Stevens CR, Blake DR: Role of the endothelial cell in osteoblast control: new perspectives. Bone 1993;14:97-102 https://doi.org/10.1016/8756-3282(93)90234-2
  20. Collin-Osdoby P: Role of vascular endothelial cells in bone biology. J Cell Biochem 1994;55:304-309 https://doi.org/10.1002/jcb.240550306
  21. Shifren JL, Doldi N, Ferrara N, Mesiano S, Jaffe R: In the human fetus, vascular endothelial growth factor is expressed in epithelial cells and myocytes, but not vascular endothelium: Implication for mode and action. J Clin Endocrinol Metab 1994;79:316-322 https://doi.org/10.1210/jc.79.1.316
  22. Carmeliet P, Ferreira V, Breier G, Pollefeyt S. Kieckens L, Gerstsenstein M, Fahrig M, Vandenhoeck A: Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature 1996;380:435-439 https://doi.org/10.1038/380435a0
  23. Dvorak HF, Orenstein NS, Calvalgo AC, Churchill WH, Dvorak AM, Galli SJ, Feder J, Bitzer AM, Rypysc J, Giovinco P: Induction of a fibrin-gel 10 investment. An early event in line 10 hepatocarcinoma growth mediated by tumor-secreted products. J Immunol 1979;122: 166-174
  24. Dvorak HF, Dvorak AM, Manseau EJ, Wiberg L, Churchill WH: Fibrin gel investment associated with line 1 and line 10 solid tumor growth, angiogenesis, and fibroplasias in guinea pigs. Role of cellular immunity, myofibroblast, microvascular damage, and infarction in line 1 tumor regression. J Natl Cancer Inst 1979;62:1459-1472
  25. Ferrara N, Leung DW, Cachianes G, Winer J, Henzel WJ: Purification and cloning of vascular endothelial growth factor secreted by pituitary folliculosatellite cells. Methods Enzymol 1991;146: 391-405
  26. Ando S, Nojima K, Majima H, Ishihara H, Suzuki M, Furusawa Y, Yamaguchi H, Koike S, Ando K, Yamauchi M, Kuriyama T: Evidence for mRNA expression of vascular endothelial growth factor by X-ray irradiation in a lung squamous carcinoma cell line. Cancer Lett 1998;23;132:75-80
  27. Sugishita Y, Takahashi T, Shimizu T, Yao A, Kinugawa K, Sugishita K, Harada K, Matsui H, Nagai R: Expression of genes encoding vascular endothelial growth factor and its Flk-1 receptor in the chick embryonic heart. J Mol Cell Cardiol 2000;32:1039-1051 https://doi.org/10.1006/jmcc.2000.1141
  28. Schweiki D, Itin A, Soffer D, Keshet E: Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature 1992;359:843-845 https://doi.org/10.1038/359843a0
  29. Claffey KP, Wilkinson WO, Spiegelman BM: Vascular endothelial growth factor. J Biol Chem 1992;267:16317-16322
  30. Eyssen-Hernandez R, Ladoux A, Frelin C: Differential regulation of cardiac heme oxygenase-1 and vascular endothelial growth factor mRNA expression by hemin, heavy metals heat shock and anoxia. FEBS Lett 1996;382:229-233 https://doi.org/10.1016/0014-5793(96)00127-5
  31. Li J, Perrella MA, Tsai JC, Yet SF, Hsieh CM, Yosizumi M, Patterson C, Endege WO, Zhou F, Lee ME: Induction of vascular endothelial growth factor gene expression by interleukin-1??in rat aortic smooth muscle cells. J Biol Chem 1995;270:308-312 https://doi.org/10.1074/jbc.270.1.308
  32. Cohen T, Nahari D, Cerem LW, Neufeld G, Levi BZ: Interleukin-6 induces the expression of vascular endothelial growth factor. J Biol Chem 1996;271:736-741 https://doi.org/10.1074/jbc.271.2.736
  33. Pertovaara L, Kaipainen A, Mustonen T, Orpana A, Ferrara N, Saksela O, Alitalo K: Vascular endothelial growth factor is induced inresponse to transforming growth factor-$\beta$in fibroblastic and epithelial cells. J Biol Chem 1994;269:6271-6274
  34. Finkenzeller G, Marme′D, Weich HA, Hug H: Platelet-derived growth factor-induced transcription of the vascular endothelial growth factor gene is mediated by protein kinase C. Cancer Res. 1992; 52:4821-4823
  35. Warren RS, Yuan H, Matli MR, Ferrara N, Donner DB: Induction of vascular endothelial growth factor by insulin-like growth factor1 in colorectal carcinoma. J Biol Chem 1996; 271:29483-29488 https://doi.org/10.1074/jbc.271.46.29483
  36. Brauchle M, Funk JO, Kind P, Werner S: Ultraviolet B and$H_{2}O_{2}$are potent inducers of vascular endothelial growth factor expression in cultured 11 keratinocytes. J Biol Chem 1996;271:21793-21797 https://doi.org/10.1074/jbc.271.36.21793
  37. Ando S, Nojima K, Majima H, Ishihara H, Suzuki M, Furusawa Y, Yamaguchi H, Koike S, Ando K, Yamauchi M, Kuriyama T: Evidence for mRNA expression of vascular endothelial growth factor by X-ray irradiation in a lung squamous carcinoma cell line Cancer Lett 1998;23;132:75-80