Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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XENOTRANSPLANT OF HUMAN BONE MARROW STROMAL CELLS; EFFECT ON THE REGENERATION OF AXOTOMIZED INFRAORBITAL NERVE IN RATS

인간 골수 기질세포 이종이식이 백서의 축삭절단 안와하 신경 재생에 미치는 효과

  • Park, Eun-Jin (Department of Dental Anesthesiology, College of Dentistry, Seoul National University) ;
  • Kim, Eun-Seok (Department of Dentistry, College of Medicine, Chungnam National University) ;
  • Kim, Jin-Man (Department of Pathology, College of Medicine, Chungnam National University) ;
  • Kim, Hyun-Ok (Department of Diagnosis and Laboratory Medicine, College of Medicine, Yonsei University) ;
  • Yum, Kwang-Won (Department of Dental Anesthesiology, College of Dentistry, Seoul National University)
  • 박은진 (서울대학교 치과대학 치과마취과학교실) ;
  • 김은석 (충남대학교 의과대학 치과학교실) ;
  • 김진만 (충남대학교 의과대학 병리학교실) ;
  • 김현옥 (연세대학교 의과대학 진단검사의학교실) ;
  • 염광원 (서울대학교 치과대학 치과마취과학교실)
  • Published : 2005.06.30
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Abstract

This study demonstrated that xenogenic human marrow mesenchymal stem cells (hMSCs) could elicit the regeneration of the sensory nerve after axotomy in the adult rats infraorbital nerves without immunosuppression. For this, we evaluated the behavioral testing for functional recovery of the nerve and histological findings at weeks 3 and 5 compared to controls. Xenogenic hMSCs did not evoke any significant inflammatory or immunologic reaction after systemic and local administrations. HMSCs-treated rats exhibited significant improvement on sensory recovery tested with von Frey monofilaments. At 5 postoperative weeks, in the hMSCs treated nerve, expression of myelin basic protein (MBP), neurofilament (NF) at the site of axotomy was higher than control. And mRNA expression of neurotropin receptor Trk precursor (TrkPre), nerve growth factor receptor (NGFR) and neuropeptide (NPY) in trigeminal ganglion were also higher. The number of myelinated nerve at distal stump and cells in trigeminal ganglion were higher in hMSC treated rats. So it was supposed that transplanted MSCs contributed to reducing post-traumatic degeneration and production of neurotrophic factors. Immunofluorescence labeling showed small portion of hMSCs (<10%) expressed a phenotypic marker of Schwann cell (S-100). Xenogenic or allogenic mesenchymal stem cells might have immune privileged characteristics and useful tool for cell based nerve repair.

Keywords

References

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