Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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In vitro propagation from axillary bud explants of Fatsia japonica Deene. et Planeh

팔손이 액아배양을 통한 기내증식

  • Choi, Kyung-Mi (Dept. of Biology and Institute of plant Resources, Chonnam Natl. Univ.) ;
  • Hwang, Sung-Jin (Dept. of Oriental Medicine Materials, Dongshin Univ.) ;
  • Ahn, Jun-Cheul (Dept. of Life Science, Seonam Univ.) ;
  • Lee, Hyeon-Yong (Dept. of School of Biotechnology & Bioengineering, Kangwon Natl. Univ.) ;
  • Kim, Jae-Heun (Dept. of School of Biotechnology & Bioengineering, Kangwon Natl. Univ.) ;
  • Hwang, Baik (Dept. of Biology and Institute of plant Resources, Chonnam Natl. Univ.)
  • 최경미 (전남대학교 생물학과) ;
  • 황성진 (동신대학교 한약재산업학과) ;
  • 안준철 (서남대학교 생명과학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 김재헌 (강원대학교 바이오산업공학부) ;
  • 황백 (전남대학교 생물학과)
  • Published : 2005.12.30
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Abstract

A propagation for Fatsia japonica using axillary bud explants were established. Cultures were initiated from axillary bud explants on MS medium supplemented with IAA $(1,2,3\;mgl^{-1})$, 2,4-D $(1,2,3\;mgl^{-1})$ or NAA $(1,2,3\;mgl^{-1})$ in combination with BA $(0.5\;mgl^{-1})$. The maximum shoot bud formation was obtained in MS medium supplemented with $0.5\;mgl^{-1}$ BA and $2;mgl^{-1}$ IAA after 4 weeks culture. The microshoot rooted within 4 week in MS medium containing $1.0\;mgl^{-1}$ IBA.

액아가 포함된 줄기절편을 $0.5\;mgl^{-1}$ BA와 함께 IAA, NAA, 2,4-D를 농도별 $(1,2,3\;mgl^{-1})$로 조합한 MS기본배지에 치상하여 신초를 유도하였다. 액아로부터 신초의 형성은 $0.5\;mgl^{-1}$ BA와 $2;mgl^{-1}$ IAA를 혼합처리한 배지에서 57%의 효율로 가장 높게 나타났다. 신초로부터 발근은 MS배지에 IBA를 $1\;mgl^{-1}$를 첨가하였을 때 가장 좋았다. 발생한 root의 길이는 평균 4주 후 약 4 cm로 측정되었다.

Keywords

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