Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Up-regulation of Asiaticoside Biosynthesis by Methyl Jasmonate and Thidiazuron in Centella asiatica L. Urban

  • Kim, Ok-Tae (Department of Biology and Institute of Plant Resources, Chonnam National University) ;
  • Kim, Min-Young (Department of Biology and Institute of Plant Resources, Chonnam National University) ;
  • Ahn, Jun-Cheul (Department of Life Sciences, Seonam University) ;
  • Hwang, Baik (Department of Biology and Institute of Plant Resources, Chonnam National University)
  • Published : 2005.10.30
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Abstract

Centella asiatica accumulates large amounts of triterpene saponin, such as centellasaponin, asiaticoside, madecassoside. We examined the effect of two candidates, MJ (Methyl jasmonate) and TDZ (thidiazuron), on asiaticoside production and the accumulation of bAS mRNA associated with asiaticoside biosynthesis in leaves of cultured whole plants. The growth of whole plants treated with 0.1 mM MJ was found to decrease significantly, however, the growth of whole plants treated with 0.1 mM MJ plus 0.025 mg/l TDZ was better than that treated with MJ alone. When MJ alone was added to culture medium, asiaticoside contents in leaves were higher than that of control after 7 days of treatments. The maximum level of bAS $({\beta}-amyrin\;synthsae)$ mRNA in leaves of whole plant treated TDZ and MJ was transiently observed after exposure to 5 days. These results showed the up-regulation of bAS gene by adding TDZ and MJ at the molecular level, however, synergic effects of TDZ and MJ on asiaticoside biosynthesis were not testified.

Keywords

References

  1. Chem. Rev. v.93 Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes Abe, I.;Rohmer, M.;Prestwich, G.D.
  2. Exp. Cell Res. v.50 Nutrient requirements of suspension culture of soybean root cells Gamborg, O.L.;Miller, R.A.;Ojima, K.
  3. Proc. Natl. Acad. Sci. USA v.98 A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots Haralampidis, K.;Bryan, G.;Qi, X.;Papadopoulou, K.;Bakht, S.;Melton, R.;Osbourn, A.
  4. Plant Cell Physiol. v.44 Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra Hayashi, H.;Huang, P.Y.;Inoue, K.
  5. Centella;Hager's Handbuch der Phannazeutischen Praxis. Band 4. Kartnig, T.;Hoffmann-Bohm, K.;Hansel, R.(ed.);Keller, K.(ed.);Schneider, Rimpler H.(ed.)
  6. Urban. J. Plant Biol. v.47 Effect of growth regulators on asiaticoside production in whole plant cultures of Centella asiatica (L.) Kim, O.T.;Kim, M.Y.;Huh, S.M.;Ahn, J.C.;Seong, N.S.;Hwang, B.
  7. Plant Cell Rep. v.23 Stimulation of asiaticoside production from Centella asiatica (L.) Urban whole plant cultures by elicitor Kim, O.T.;Kim, M.Y.;Hong, M.H.;Ahn, J.C.;Hwang, B.
  8. Urban. J. Plant Biol. Isolation and characterization of squalene synthase cDNA from Centella asiatica (L.) Kim, O.T.;Ahn, J.C.;Hwang, S.J.;Hwang, B.
  9. Urban. Plant Cell Rep. v.24 Cloning of a cDNA probably encoding oxidosqualene cyclase associated with asiaticoside biosynthesis from Centella asiatica (L.) Kim, O.T.;Kim, M.Y.;Huh, S.M.;Bai, D.G.;Ahn, J.C.;Hwang, B.
  10. Urban. Biotechnol. Bioprocess Eng. v.10 Cloning and molecular analysis of cDNA encoding cycloartenol synthase from Centella asiatica (L.) Kim, O.T.;Kim, M.Y.;Ahn, J.C.;Hwang, S.J.;Hwang, B.
  11. Chem. Pharm. Bull. v.49 Medicinal Foodstuffs. XXVII. Saponin constituents Gotu Kola (2): Structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponin B, C, and D, from Centella asiatica cultivated in Sri Lanka Matsuda, H.;Morikawa, T.;Ueda, H.;Yoshikawa, M.
  12. Trends Plant Sci. v.6 Organization of jasmonte-responsive gene expression in alkaloid metabolism Memelink, J.;Verpoorte, R.;Kijne, J.W.
  13. Proc. Natl. Acad. Sci. USA v.96 Compromised disease resistance in saponin-deficient plants Papadopoulou, K.;Melton, R.E.;Leggett, M.;Daniels, M.J.;Osbourn, A.E.
  14. Molecular cloning: a laboratory manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.
  15. J. Plant Physiol. v.129 The effect of methyl jasmonate on ethylene production and ethylene-forming enzyme activity in tomatoes Saniewski, M.;Nowacki, J.;Czapski, J.
  16. C R Acad. Sci. Series v.293 Le jasmonate de methyle: Nouveau et puissant promoteur de la senescence des feuilles Satler, S.O.;Thimann, K.V.
  17. Plant Cell Rep. v.17 Interaction of methyl jasmonate, wounding and fungal elicitation during sesquiterpene induction in Hyoscyamus muticus in root cultures Singh, G.;Gavrieli, J.;Oakey;Curtis, W.R.
  18. The Plant J. v.32 A genomic approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula Suzuki, H.;Achnine, L.;Xu, R.;Matsuda, S.P.T.;Dixon, R.A.
  19. Plant Cell Rep. v.18 Fungal elicitor perparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei Szabo, E.;Thelen, A.;Petersen, M.
  20. Physiol. Plant v.69 Degradation of ribulose-1,5-biphosphate carboxylase and chlorophyll in senescing barley leaf segments tirggered by jasmonic acid methyl ester and counteraction by cytokinin Weidhase, R.A.;Lehmann, J.;Kramell, H.;Sembder, G.;Parthier, B.
  21. Appl. Microbiol. Biotechnol. v.55 Improved ${\beta}$-thujaplicin production in Cupressus lusitanica suspension cultures by fungal elicitor and methyl jasmonate Zhao, J.;Fujita, K.;Yamada, J.;Sakai, K.