Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Genetic Relationships in Hypericum erectum Thunb. by RAPD

RAPD 방법을 이용한 고추나물의 유연관계분석

  • Kim, Sun-Hee (Department of Biology, Chonnam National University) ;
  • Kim, Eung-Sik (Department of Life Sciences, Seonam University) ;
  • Kim, Sung-Ho (Department of Life Sciences, Seonam University) ;
  • Ahn, Jun-Cheul (Department of Life Sciences, Seonam University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • Published : 2005.08.30
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Abstract

The genetic relationships of Hypericum erectum, H. ascyron and H. perforatum Thunb. by RAPD using total 46 primers were analysed 30 primers among primers tested showed the amplification band in all. The amplified DNA fragment ranged from 0.25 to 6.6 kb in size. The 411 bands (34.4%) among 1194 bands derived from 30 primers were polymorphic, and 13.7 bands per primer were observed. The phenograms for six analyzed individuals by RAPD markers were not matched well with those of the result by morphological characters. They were clustered monophyletic at the similarity coefficient value ranged from 0.24 to 0.96.

Random amplified polymorphic DNAs (RAPD) 분석을 통해 한국산 물레나물 속 (Hypericum) 식물에서의 유연관계를 분석한 결과, 병풍산 고추나물과 지리산 고추나물 사이에 상당한 유전적 차이가 있음을 확인할 수 있었고, 지리산의 500m, 1000m 그리고 1300m 고도 차이에 따른 군락지별로도 밴드 패턴의 차이가 확인되었다. 동일 과(科)에 속한 물레나물보다는 서양고추나물이라고 불리는 St. John's wort가 고추나물과 유연관계가 더 가깝게 나타났다.

Keywords

References

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