한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제13권3호
  • /
  • Pages.105-108
  • /
  • 2005
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

동백 자엽절편으로부터 부정아 형성을 통한 식물체의 재분화

Adventitious Bud Induction and Plant Regeneration from Cotyledon Explants of Camellia japonica L.

  • 김광수 (동신대학교 생물자원산업화지원센터) ;
  • 황성진 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 김선민 (동신대학교 한약재산업학과)
  • 발행 : 2005.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

기내배양을 통해 동백종자의 자엽절편으로부터 직접 체세포분화를 통하여 식물체를 재분화 시켰다. 종피를 제거한 후 표면살균 처리한 자엽절편을 2.4-D, NAA 및 BA가 단독 또는 조합 첨가된 MS 고체배지에 치상하였다. 원배체 형성을 통한 부정아 형성은 0.1 mg/l 2.4-D와 1 mg/l BA가 첨가된 처리구에서 이루어졌다. 부정아는 주로 자엽절편체의 배축에 가까운 기저부위에서 캘러스의 형성없이 직접 형성되었으며, 이로부터 형성된 유묘는 식물생장조절물질이 첨가되지 않은 기본배지에서 정상적인 개체로 발달 하였다. 이와같은 연구결과는 앞으로 동백나무의 육종과 산업적 이용에 필요한 형질전환 연구에 효과적으로 활용될 수 있을 것으로 사료된다.

Culture conditions for plant regeneration of Camellia japonica were achieved by organogenesis in explants of cotyledon. Seed cotyledons were cultured on MS medium containing various auxin, 2,4-D or NAA and cytokinins BA. The adventitious shoot buds were efficiently formed without embryogenesis on the basal region of cotyledon cultured on MS medium supplemented with $0.1\;{\mu}g/m{\ell}$ 2.4-D and $1\;{\mu}g/m{\ell}$ BA. Seed cotyledons could be used as a source of explants in experiments of genetic transformation of the genotypes evaluated for improving the efficiency of production of transgenic Camellia plants.

키워드

참고문헌

  1. Plant Cell Reports v.19 High-yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, 'TRI-2025', by temporary immersion Akula, A.;Becker, D.;Bateson, M.
  2. Plant Cell, Tissue and Org. Culture v.40 Genotype and medium affect shoot regeneration of melon Ficcadenti, N.;Rotino, G.L.
  3. Plant Cell, Tissue and Org. Culture v.20 An in vitro technique for the production de novo of multiple shoots in cotyledon explants of Cucumis sativa L. Gambley, R.L.;Dodd, W.A.
  4. Plant Cell Physiol. v.15 Bud formation on excised Heloniopsis leaf fragment: Effects of leaf age and the midrib Kato, Y.
  5. Korean J. Plant Tissue Culture v.24 no.6 Plant regeneration and somatic embryogenesis from zygotic embryo-derived callus of native Prunus yedoensis in Mt. Halla Koh, J.G.;Park, Y.C.;Yang, D.Y.;Kim, E.S.;Oh, M.Y.;Koh, S.C.
  6. Nat. Biotechnol. v.14 Genetic transformation of Manihot esculenta Crantz Li, H.Q.;Sautter, C.;Potrykus, I.;Puonti-Kaerlas, J.
  7. New Phytol. v.95 Cytokinins and the regeneration of plantlets from secondary embryoids of winter oilseed rape, Brassica napus spp. oleifera Loh, C.S.;Ingram, D.S.;Hanke, D.E.
  8. Plant Growth Regulation v.26 Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron Mondal, T.K.;Bhattacharya, A.;Sood, A.;Ahuja, P.S.
  9. Plant Cell Reports v.20 Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangra Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos Mondal, T.K.;Bhattacharya, A.;Ahuja, P.S.;Chand, P.S.
  10. Physil. Plant v.15 A revised medium for rapid growth and bioassay with tabacco tissue Murashige, T.;Skoog, F.
  11. Plant Science v.96 Early detection of embyogenic competence and polarity in Camellia japonica L. by electron probe X-ray microanalysis Pedroso, M.C.;Pais, M.S.
  12. Plant Cell Reports v.16 Somatic embryogenesis and plant regeneration from the immature cotyledonary tissues of cultivated tea (Camellia sinensis (L.) O. Kuntze) Ponsamuel, J.;Samson, N.P.;Ganeshan, P.S.;Sathyaprakash, V.;Abrsham, G.C.
  13. Plant Cell, Tissue and Organ Culture v.65 An efficient liquid culture system for tea shoot proliferation Sandal, I.;Bhattacharya, A.;Ahuja, P.S.
  14. Plant Cell Rep. v.20 In vitro regeneration in Primula ssp. via organogenesis Schween, G.;Schwenkel, H.G.
  15. Agri. Biol. Chem. v.48 no.11 Occurrence of tanine in Camellia japonica and Camellia sasanqua Tsushida, T.;Takeo, T.
  16. Plant Cell Tissue, & Organ Culture v.21 Somatic embryogenesis and plant regeneration from embryonic tissue of Camellia japonica L. Vieitez, A.M.;Barciela, J.