한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제13권2호
  • /
  • Pages.95-102
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  • 2005
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

Protection of Amyloid ${\beta}$ Protein (25-35)-induced Neuronal Cell Damage by Methanol Extract of New Stem of Phyllostachys nigra Munro var. henonis Stapf in Cultured Rat Cortical Neuron

  • Ban, Ju-Yeon (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.) ;
  • Cho, Soon-Ock (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.) ;
  • Kwon, Soon-Ho (College of Agriculture & Life-Sci., Kyungpook Natl. Univ.) ;
  • Kim, Jin-Bae (College of Oriental Medicine, Kyung Hee Univ.) ;
  • Song, Nak-Sul (National Institute of Crop Science, RDA.) ;
  • Bae, Ki-Whan (College of Pharmacy, Chungnam Natl. Univ.) ;
  • Song, Kyung-Sik (College of Agriculture & Life-Sci., Kyungpook Natl. Univ.) ;
  • Seng, Yeon-Hee (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.)
  • 발행 : 2005.04.30
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초록

Caulis Bambusae in Taenia is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of Caulis Bambusae in Taenia (CB) from Phyllostachys nigra Munro var. henonis Stapf (Gramineae) on amyloid ${\beta}$ protein (25-35) $(A{\beta}\;(25-35))$, a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. CB, over a concentration range of $10-50{\mu}g/{\mu}l$, inhibited the $A{\beta}\;(25-35)\;(10\;{\mu}M)$-induced neuronal cell death, as assessed by a 3-[4,5-dimethyIthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. CB $(50\;{\mu}g/{\mu}l)$ inhibited glutamate release into medium induced by $10\;{\mu}M\;A{\beta}$, (25-35) which was measured by HPLC. Pretreatment of CB $(50\;{\mu}g/{\mu}l)$ inhibited $10{\mu}M\;A{\beta}$ (25-35)-induced elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, fluo-4 AM, and generation of reactive oxygen species. These results suggest that CB prevents $A{\beta}$ (25-35)-induced neuronal ell damage in vitro.

키워드

참고문헌

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