Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of Artemisia anomala S. Moore on Antioxidant Activity and Melanogenesis

유기노의 항산화 활성 및 멜라닌 생성 억제 효과

  • 이범천 (한불화장품(주) 기술연구소) ;
  • 김진화 (한불화장품(주) 기술연구소) ;
  • 김진희 (한불화장품(주) 기술연구소) ;
  • 표형배 (한불화장품(주) 기술연구소) ;
  • ;
  • 박흠대 (대구대학교 공과대학 생물공학과) ;
  • 조영호 (한불화장품(주) 기술연구소)
  • Published : 2005.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

In mammalian melanocytes, melanin synthesis is controlled by tyrosinase, the key enzyme in the pigment synthesis. In this study, to develop a new whitening agent, we have investigated the antioxidant and the inhibitory effect of Artemisia anomala extract on tyrosinase activity and melanigenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of butanol fraction from A. anomala was higher than that of arbutin ($97.5{\pm}0.5%$ at the concentration of 2 mg/ml). The butanol fraction was shown scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also butanol fraction ($25.0{\pm}3%$ at the concentration of $200\;{\mu}g/ml$). From these results, we suggest that the A. anomala extract might be used to be a potential agent for skin whitening.

Keywords

References

  1. 구본홍(1995) 한글동의보감, 1436. 한국교육문화사, 사울
  2. 장일무(2003), 동양의약과학대전 I, 327. 서울대학교 천연물과학연구소 문헌정보학연구실. 서울
  3. Lee, J. K., Jung, H. M. and Kim, S. Y. (2003) 1,8-Dihydroxynaphthalene (DHN)-melanin biosynthesis inhibitors increase erythritol production in Torula corallina, and DHNmelanin inhibits erythrose reductase. Appl. Envir. Microbiol. 69: 3427-3434 https://doi.org/10.1128/AEM.69.6.3427-3434.2003
  4. Hill, H. Z., Li, w., Xin, P. and Mitchell, D. L. (1997) Melanin: a two edged sword? Pigment Cell Res. 10: 158-161 https://doi.org/10.1111/j.1600-0749.1997.tb00478.x
  5. Marmol, V. del. and Beermann, F. (1996) Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381: 165-168 https://doi.org/10.1016/0014-5793(96)00109-3
  6. Hearing, V. J. (1999) Biochemical control of melanogenesis and melanosomal organization. J. Investig. Dermatol. Symp. Proc. 4: 24-28
  7. Chakraborty, A. K, Funasaka, Y., Komoto, M. and Ichihashi, M. (1998) Effect of arbutin on melanogenic proteins in human. Pigment Cell Res. 11: 206-212 https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  8. Cabanes, J., Chazarra, S. and Garci-Carmona, F. (1994) Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol. 46: 982-985 https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  9. Fujiware, Y., Sahashi, Y., Artro, M., Hasegawa, S., Akimoto, K., Ninomiya, S., Sakaguchi, Y. and Seyama, Y. (2004) Effect of simultaneous administration of vitamin C, L-cysteine and vitamin E on the melanogenesis. Biofactors. 21: 415-418 https://doi.org/10.1002/biof.552210182
  10. Lee, S. H., Choi, S. Y., Kim, H., Hwang, J. S., Lee, B. G, Gao, J. J. and Kim, S. Y. (2002) Mulberroside F isolated from the leaves of Morus alba inhibits melanin synthesis. Biol. Pharm. Bull. 25: 1045-1048 https://doi.org/10.1248/bpb.25.1045
  11. Jung, G. D., Yang, J. Y., Song, E. S. and Par, J. W. (2001) Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells. Exp. Mol. Med. 33: 131-135 https://doi.org/10.1038/emm.2001.23
  12. No, J. K, Soung, D. Y., Kim, Y. J., Shim, K. H., Jun, Y. S., Rhee, S. H., Yokozawa, T. and Chung, H. Y. (1999) Inhibition of tyrosinase by green tea components. Life Sciences. 65: 241-246
  13. Lee, K. E., Sim, K. S., Kim, J. H., Park, S. M., Lee, B. C., Yun, Y. P, Zhang, Y. H. and Pyo, H. B. (2004) Effects of the Scirpi rhizoma on antioxidation and melnogenesis. Yakhak Hoeji 48: 323-327
  14. Choi, B. W., Lee, B. H., Kang, K J., Lee, E. S. and Lee, N. H. (1998) Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Kor. J. Pharmacogn. 29: 237-242
  15. Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical. Nature. 181: 1199 https://doi.org/10.1038/1811199a0
  16. Furuno, K., Akasako, T. and Sugihara, N. (2002) The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull. 25: 1923
  17. Mosmann, T. (1983) Rapid colorimetric assay forthe cellular growth and survival: application to proliferation and cytotoxic assay. J. Immunol. Methods. 65: 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  18. Hosoi, J., Abe, E., Suda, T. and Kuroki, T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478
  19. Kuppusamy, P. and Zweier, J. L. (1989) Characterization of free radical generation by xanthine oxidase. J. Biol. Chem. 264: 9880-9884
  20. Englaro, W., Rezzonico, R., Durand-Clement, M., Lallemand, D., Ortonne, JP. and Ballotti, R. (1995) Mitogen-activated protein kinase pathway and AP-l are activated during cAMP-induced melanogenesis in B-16 melanoma cells. J. Biol. Chem. 270: 24315-24320 https://doi.org/10.1074/jbc.270.41.24315