Inhibitory Effects of the Seed Extract of Myristica fragrans on the Proliferation of Human Tumor Cell Lines

육두구 추출물의 암세포증식 저해 효과

  • Published : 2005.09.30

Abstract

The methanol extract of the seed of Myristica fragrans (myristicaceae) demonstrated a potent inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2(melanoma), XF498 (central nerve system) and HCT-15(colon). The MeOH extract was fractionated into three portions by serial solvent partition i,e., EtOAc soluble part, BuOH soluble part and remaining water layer. Among them, the EtOAc soluble part of the extract demonstrated a potent inhibition on the proliferation of cultured human tumor cells, Bioassay-guided fractionation of the EtOAc soluble part led to the isolation of six lignan constituents, i.e., safrole(1), machilin A (2), licarin B (3), macelignan (4), mesodihydroguaiaretic acid (5) and myristargenol A (6) as well as a large amount of myristic acid as active ingredients. Structures of the isolated active components (1-6) were established by chemical and spectroscopic means.

Keywords

References

  1. 안덕균(1999) 한국본초도감, 755. 교학사
  2. 한국의약품수출입협회(2000) 한국의약품시험연구소편 한약(생약)규정집, 370
  3. Skehan, P., Storeng, R., Schudiero, D. A., Monks, A., McMahn, J., Vistica, D., Warren, J. T., Bokesch, H., Kenny, S. and Boyd, M. R. (1990) New colorimetric cytotoxicity assay for anticancer drug screening. J. Natl. Cancer Inst. 82: 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  4. Kim, K. J. and Han, Y N. (2002) Lignans from Myristica Fragrans. Yakhak Hoeji. 46: 98-101
  5. Shimomura, H., Sashida, Y and Oohara, M. (1987) Lignans from Machilus Thunbergii. Phytochemistry. 26: 1513-1515 https://doi.org/10.1016/S0031-9422(00)81847-6
  6. Enriquez, R. G. and Chavez, M. A. (1984) Phytochemical investigations of plants of the genus Aristolochia, 1. isolation and nmr spectral characterization of eupomatenoid derivatives. J. Nat. Prod. 47: 896-899 https://doi.org/10.1021/np50035a030
  7. Kim, Y. B., Park, I. Y. and Shin, K. H. (1991) The crystal structure of licarin B (C20H2004) a component of the seed of Myristica Fragrans. Arch. Pharm. Res. 14: 1-6 https://doi.org/10.1007/BF02857805
  8. Shin, K. H., Kim, O. N. and Woo, W. S. (1988) Isolation of hepatic drug metabolism inhibitors from the seeds of Myristica Fragrans. Arch. Pharm. Res. 11: 240-243 https://doi.org/10.1007/BF02861315
  9. Woo, W. S., Shin, K. H., Wagner, H. and Lotter, H. (1987) The structure of macelignan from Myristica Fragrans. Phytochemistry. 26: 1542-1543 https://doi.org/10.1016/S0031-9422(00)81858-0
  10. Shin, K. H. and Woo, W. S. (1986) Biological evaluation of drug metabolism modifying activity. Kor. J. Pharmacogn. 17: 189-194
  11. Lee, J. Y., Han, Y. B., Woo, W. S. and Shin, K. H. (1990) Antioxidant activity of diarylbutanes. Kor. J. Pharmacogn. 21: 270-273
  12. Ikeya, Y., Taguchi, H., Yosioka, I. and Kobayashi, H. (1979) The constituents of Schizandra chinensis baill. IV The structures of two new lignans, pre-gomisin and gomisin J. Chem. Pharm. Bull. 27: 1583-1588 https://doi.org/10.1248/cpb.27.1583
  13. Nakatani, N., Ikeda, K., Kikuzaki, H., Kido, M. and Yamaguchi, Y. (1988) Diaryldimethylbutane lignans from Myristica Argentea and their antimicrobial action against Streptococcus Mutans. Phytochemistry . 27: 3127-3129 https://doi.org/10.1016/0031-9422(88)80013-X
  14. Choi, Y. H., Seo, J. H., Kim, J. S., Heor, J., Kim, S. K., Choi, S. U., Kim, Y. S., Kim, Y. K. and Ryu, S. Y. (2003) Inhibitory effects of the stem bark extract of Eucommia ulmoides on the proliferation of human tumor cell lines. Kor. J. Pharmacogn. 34: 308-313