Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidant Activity of Spatholobus suberectus Dunn

계혈등의 항산화 활성

  • Lee, Eun-Hee (Department of Bio-Industry and Technology, Sangji University) ;
  • Noh, Mi-Ae (Department of Bio-Industry and Technology, Sangji University) ;
  • Cha, Bae-Cheon (Department of Bio-Industry and Technology, Sangji University)
  • 이은희 (상지대학교 생명자원과학대학 바이오산업공학과) ;
  • 노미애 (상지대학교 생명자원과학대학 바이오산업공학과) ;
  • 차배천 (상지대학교 생명자원과학대학 바이오산업공학과)
  • Published : 2005.03.30
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Abstract

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product obtained in the aerobic metabolism. A major portion of living organisms has defense system as superoxide dismutase or catalase against damage produced by ROS. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. Spatholobus suberectus Dunn has been known as a Korean folk medicine for promoting blood circulation and relieving blood stasis as anemia. In this study, we have investigated the antioxidant activities of Spatholobus suberectus Dunn in order to find the antioxidant substances from natural products. For various antioxidant experiment, the major antioxidant component was isolated from EtOAc extract of Spatholobus suberectus Dunn. This compound was identified as (-)-epicatechin by chemical and physical analysis.

Keywords

References

  1. Kim, J. G. and Cho, B. G. (1995) Traditional drugs of the east, 150-151. Young Lim Sa, Seoul
  2. Choi,J. S., Song, T. W. and Kim, D. H. (2003) A Study on the effect of Spatholobus suberectus Dunn on the inhibition of arthritis induced by collagen on the mouse. Kor. J. Herbology, 18: 79-88
  3. Seo, H. G., Oh, Mi. S. and Kim, D. H. (2003) Immunity Responses of the Spatholubus suberectus Dunn to the Synovial Cells Isolated from Patients with Rheumatoid Arthritis. Korean. J. Oriental Physiol. Pathol. 17: 780-786
  4. Shin, S. M., Park, J. H., Yoo, D. T. and Kim, D. H. (2003) Inhibitory effect of gamihwalhyeol-tang on inflammatory cytokine and NF-B, AP-1 activation in human synovial cells. Korean. J. Oriental Physiol. Pathol. 17: 165-176
  5. Li, R. W., Lin, G. D., Myers, S. P. and Leach, D. N. (2003) Anti-inflammatory activity of Chinese medicinal vine plants. Journal of Ethnopharmacol. 85: 61-67 https://doi.org/10.1016/S0378-8741(02)00339-2
  6. Wang, W., Wang, J., Zhao, D., Liu, H., Zhou, W. and Chen, K. (1991) Comparison of Spatholobus suberectus Dunn, Euonymus alatus (Thunb.) Sied. and Eupolyphaga sinensis Walker on regulation of plasma lipid. Zhongguo Zhong Yao Za Zhi. 16: 299-301
  7. Lam, T. L., Lam, M. L., Au, T. K., Ip, D. T. M., Ng, T. B., Fong, W. P. and Wan, D. C. C. (2000) A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs. Life Sci. 67: 2889-2896 https://doi.org/10.1016/S0024-3205(00)00864-X
  8. Yoon,J. S., Sung, S. H., Park, J. H. and Kim, Y. C. (2004) Flavonoids from Spatholobus suberectus. Arch. Pharm. Res. 27: 589-592 https://doi.org/10.1007/BF02980154
  9. Fukuyama, Y., Nakano, Y., Geng, P. W., Rui, W., Sumitomo, J., Bao, J. X. and Nakagawa, K. (1988) In vitro fibrinolytic phytosterols isolated from the roots of Spatholobus suberectus. Planta Med. 54: 34-36 https://doi.org/10.1055/s-2006-962325
  10. Papa, S. and Skulachev, V. P. (1997) Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell Biochem. 174: 305-319 https://doi.org/10.1023/A:1006873518427
  11. Fukuzawa, K. and Takaishi, Y. (1990) Antioxidants. J. Act. Oxyg. Free Rad. 1: 55-70
  12. Halliwell, B. (1991) Drug antioxidant effects. Drugs, 42: 569-605 https://doi.org/10.2165/00003495-199142040-00003
  13. Cerutti, P. A. (1994) Oxy-radicals and cancer. Lancet. 344: 862-863 https://doi.org/10.1016/S0140-6736(94)92832-0
  14. Corl, M. M. (1974) Antioxidant activity of tocopherol and ascorbylpalmitate and their mode of action. JAOCS. 51: 321-325 https://doi.org/10.1007/BF02633006
  15. Coleman, M. D., Fernandes, S. and Khanderia, L. A. (2003) A preliminary evaluation of a novel method to monitor a triple antioxidant combination (vitamin E, C and $\alpha$-lipoic acid) in diabetic volunteers using in vitro methaemoglobin formation. Environ. Toxicol. Pharmacol. 14: 69-75 https://doi.org/10.1016/S1382-6689(03)00027-9
  16. Monsen, E. R. (2000) Dietary reference intakes for the antioxidant nutrients: vitamin C, vitamin E, selenium, and carotenoids. J. Am. Diet. Assoc. 100: 637-640 https://doi.org/10.1016/S0002-8223(00)00189-9
  17. Ito, N., Fukushima, S., Hagiwara, A., Shibata, M. and Ogiso, T. (1983) Carcinogenicity of butylated hydroxyanisole on F 344 rats. J. Natl. Cancer Inst. 70: 343-352
  18. Branen, A. L. (1975) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. JAOCS. 52: 59-63 https://doi.org/10.1007/BF02901825
  19. Yu, W., Zhao, Y. and Shu, B. (2004) The radical scavenging activities of radix puerariae isoflavonoids. Food Chem. 86: 525-529 https://doi.org/10.1016/j.foodchem.2003.09.005
  20. Choi, S. I., Lee, Y. M. and Heo, T. R. (2003) Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean. J. Biotechnol. Bioeng. 18: 282-288
  21. Fukumoto, L. R. and Mazza, G. (2000) Assessing antioxidant and peroxidant activities of phenolic compounds. J. Agric. Food Chem. 48: 3597-3604 https://doi.org/10.1021/jf000220w
  22. Lim, D. K., Choi, U. and Shin, D. H. (1996) Antioxidative activity of ethanol extract from Korean medicinal plants. Korean. J. Food Sci. Technol. 28: 83-89
  23. Uchiyama, M., Suzuki, Y and Fukuzawa, K. (1968) Biochemical studies of physiological function of tocopheronolactone. 1. Yakugaku Zasshi, 88: 678-683 https://doi.org/10.1248/yakushi1947.88.6_678
  24. Yoshikawa, M., Harada, E., Miki, A, Tsukamoto, K., Liang, S. Q., Yamahara, J. and Murakami, N. (1994) Antioxidant constituents from the fruit hulls of Mangosteen (Garcinia mangostana L.) originating in vietnam. Yakugaku Zasshi, 114: 129-133 https://doi.org/10.1248/yakushi1947.114.2_129
  25. Inatani, R., Nakatani, N. and Fuwa, H. (1983) Antioxidative effect of the constituents of rosemary(Rosemaries officinalis L.) and their derivatives. Agric. Bioi. Chem. 47: 521-528 https://doi.org/10.1271/bbb1961.47.521
  26. Chen, C. W. and Ho, C. T. (1995) Antioxidant properties of polyphenols extracted from green and black teas. J. Food Lipids, 2: 35-46 https://doi.org/10.1111/j.1745-4522.1995.tb00028.x
  27. Lim, D. K., Choi, U. and Shin, D. H. (1996) Antioxidative of some solvent extract from Caesalpinia sappan L. Korean J. Food Sci. Technol. 28: 77-82
  28. Natsume, M., Osakabe, N., Oyama, M., Sasaki, M., Baba, S., Nakamura, Y, Osawa, T. and Terao, J. (2003) Structure of (-)-epicatechin glucuronide identified from plasma and urine after oral ingestion of (-)-epicatechin : differences between human and rat. Free Radical BioI. Med. 34: 840-849 https://doi.org/10.1016/S0891-5849(02)01434-X
  29. Berregi, I., Santos, J. I., Campo, G. and Miranda, J. I. (2003) Quantitative determination of (-)-epicatechin in cider apple juices by $^1H$ NMR. Talanta, 61: 139-14 https://doi.org/10.1016/S0039-9140(03)00236-4
  30. Cren-Olive, C., Wieruszeski, J. M., Maes, E. and Rolando, C. (2002) Catechin and epicatechin deprotonation followed by $^{13}C$ NMR. Tetrahedron Lett. 43: 4545-4549 https://doi.org/10.1016/S0040-4039(02)00745-1