Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Phytochemical and Pharmacological Investigations on Moringa peregrina (Forssk) Fiori

  • Elbatran, Seham A. (Departments of Pharmacology, Chemistry of Medicinal Plants, National Research Centre) ;
  • Abdel-Salam, Omar M. (Departments of Pharmacology, Chemistry of Medicinal Plants, National Research Centre) ;
  • Abdelshfeek, Khaled A. (Chemistry of Medicinal Plants, National Research Centre,Chemistry Department, Faculty of Science, Al-Tahady University) ;
  • Nazif, Naglaa M. (Chemistry of Medicinal Plants, National Research Centre) ;
  • Ismail, Shams I. (Chemistry of Medicinal Plants, National Research Centre) ;
  • Hammouda, Faiza M. (Chemistry of Medicinal Plants, National Research Centre)
  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Investigation of M. peregrina aerial parts revealed the isolation and identification of 4-flavonoidal compounds, quercetin, quercetin-3-0-rutinoside (rutin), chrysoeriol-7-0-rhamnoside 6,8,3',5'-tetramethoxy apigenin. The compounds were identified by TLC, PC, MS, and $H^1-NMR$. The fatty acids and unsaponifiable matter were studied. The $LD_{50}$ for M. peregrina was 113.4 mg/100g b.wt. Repeated intraperitoneal injection of 1/20 and 1/10 $LD_{50}$ (5.67 mg and 11.34 mg/100g b.wt.) of defatted alcoholic of M. peregrina for 30 days induced significant decrease in serum glucose, liver enzymes and lipid components. M. peregrina administered i.p., 30min prior to carrageenan at the above doses significantly inhibited the rat paw oedema response, In acute pain models, namely, the acetic acid-induced writing and hot-plate assay, M. peregrina exhibited marked analgesic properties. In addition, M. peregrina administered at time of indomethacin injection inhibited the development of gastric lesions in rats.

Keywords

References

  1. Alcaraz, M.J. and Jimenez, M.J., Flavonoids as anti-inflammatory agents. Fitoterapia 59, 25-38 (1988)
  2. Alkahtani, H.A. and Abu -Arab, A.A., Comparison of physical, chemical, and functional properties of M. peregrina (AL-Yassar or AL-Ban ) and soybean proteins. Cereal chemistry 70 (6), 619-626 (1993)
  3. Armitage, P., Statistical Methods in Medical Research. Blackwell Scientific Publications, London 1971
  4. Ashok-Kumar, N. and Pari, L., Antioxidant action of Moringa oleifera lam (drumsticks) against antitubercular drug induced lipid peroxidation in rats. J. Med. Food 6 (3), 255-9 (2003) https://doi.org/10.1089/10966200360716670
  5. Batanouny, K. H., Shabana, M., Aboutabl, E., and Soliman, F., Wild Medicinal Plants in Egypt. Palm press, Cairo, Egypt, 1999, pp. 151
  6. Bernard, F., Juma, Abiy Yeneseq, Jacob, O. Midiwo, Peter, O. Waterman, Flavones and phenylpropenoids in the surface exudates of Psiadia punctulata. Phytochemistry 57, 571-574 (2001) https://doi.org/10.1016/S0031-9422(01)00147-9
  7. El-Wakkad, A.S., Ibrahim, S., and Mannaa, F., Vitamin E. supplementation and oxidative stress in a treptozotocin induced diabetic rats. Arab J. Lab. Med., 26 (3), 297-304 (2000)
  8. Ferrel, J.E., Chang-sing, P.D., Loew, G., King, R., Mansour, J.M., and Mansour, T.E., Structure/activity studies of flavonoids as inhibitors of cAMP phosphodiesterase and relationship to quantum chemical indices. Mol Pharmacol 16, 556 (1979)
  9. Fossatip, P., Enzymatic determination of serum triglycerides Principle. Clin. Chem. 28, 2077 (1982)
  10. Furst, P., Antioxidative power of non-nutritive substances in foodstuffs. Ernahrung 19, 457-460 (1995)
  11. Ghasi, S., Nwobodo, E., and Ofili J.O., Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high fat diet fed wistar rats. J. Ethnopharmacol. 69 (1), 21-5 (2000) https://doi.org/10.1016/S0378-8741(99)00106-3
  12. Glombitza, K.W., Mahran, G.H., Mirhom, Y.W., Michel K.G., and Motawi, T.K., Hypoglycemic and antihyperglycemic effects of Zizyphus spina-christi in rats. Planta Med. 60, 244-247 (1993) https://doi.org/10.1055/s-2006-959468
  13. Kar, A., Choudhary, B.K., and Bandyopadhyay, N.G., Comparative evaluation of hypoglycemic activity of some Indian medicinal plants alloxan diabetic rats. J. Ethnopharmacol 84, 105-8 (2003) https://doi.org/10.1016/S0378-8741(02)00144-7
  14. Karber, G., Bietrag Zur Kollectiven behandlung Pharmakolgischem reinhen versuche. Arch Exp. Pathol. Pharamakol. 162, 480-482 (1931) https://doi.org/10.1007/BF01863914
  15. Kind, P.R.N. and King, E.J., Colorimetric determination of alkaline phosphatase. J. Clin. Path. 7, 322 (1954)
  16. Korthuis, R.J., Benoit J.N., and Kvietys; P.R., Intestinal hypermia in experimental diabetes mellitus. Am. J. Physiol. 253, G26-G32 (1987)
  17. Koster, R., Anderson, M., and De Beer, E.J., Acetic acid for analgesic screening. Fed Proc. 18, 412 (1959)
  18. Mabry, T.J., Markham, K.R., and Thomas, M.B., The systematic identification of flavonids. Springer-Verlag, Berlin-Heideberg New York (1970)
  19. Markham, K.R. Techniques of Flavonoid Identification. Academic press, A subsidiary of Harcourt Brace, Jouvanovich publishers (1982)
  20. Mehta, K., Balaraman, R., Amin, A.H., Bafna, P.A., and Gulati O. D., Effect of fruits of Moringa olifera on the lipid profile of normal and hypercholesterolaemic rabbits. J. Ethnopharmacol 86, 191-5 (2003) https://doi.org/10.1016/S0378-8741(03)00075-8
  21. Mekonnen, Y., Yardely, V., Rock, P., and Craft, S., In vitro antitriptosomal activity of M. stenopetala leaves and roots. Phytotherapy Res. 13, 60:538-39 (1999) https://doi.org/10.1002/(SICI)1099-1573(199909)13:6<538::AID-PTR486>3.0.CO;2-K
  22. Millan, M.J., Bervoets, K., Rivet, J-M., Widdowson, P., Renouard, A., Le Marouille-Girardon, S., and Gobert, A., Multiple alpha-adrenergic receptor subtypes. II. Evidence for a role of rat $R_{2A}$-ARs in the control of nociception, motor behaviour and hippocampal synthesis of noradrenaline. J. Pharmacol. Exp. Ther. 270, 958-972 (1994)
  23. Miller, A.G. and Morris, M., Plants of Dhofar, the Southern Region of Oman. Traditional economic and medicinal uses. The office of the Advisor for Conservation of the Environment, Diwan of Royal Court, Sultanate of Oman 1989
  24. Mossa, J.S., EI Domiaty, M.M., AI Meshal, L.A., Hufford, C.D., and Mcphail, R.D., A flavone and diterpenes from Psiadia Arabica. Phytochem 31, 2863-2868 (1992) https://doi.org/10.1016/0031-9422(92)83648-I
  25. Mozsik, Gy., Moron, F., and Javor, T., Cellular mechanisms of the development of gastric mucosal damage and of gastric cytoprotection induced by prostacyclin in rats. A pharmacological study
  26. Murrary, R.K., Granner, D.K., Mayes, P.A., and Rodwell, V.W., Harper's Biochemistry, 23rd ed. Appleton Lange, Norwalk, San Mateo, California 1993
  27. Reitman, S. and Frankel, S., Colorimetric method for aspirate and alanine tranferases. Am. J. Clin. Pathol. 28, 56-63 (1957)
  28. Porsolt, R.D., Le Pichon, M., and Jalfre, M., Depression: a new animal model sensitive to antidepressant treatments. Nature, 266, 730-732 (1977) https://doi.org/10.1038/266730a0
  29. Radwan, A.G. Studies on histamine metabolism in the rat. Ph.D. Thesis in Pharmacology. London University (1967)
  30. Richmond, W., Preparation and properties of a cholesterol oxidase from norcardia sp and its application to the enzymatic assay of total cholesterol in serum, Clin. Chem. 19, 1350 (1973)
  31. Shah, A.A., Fitzgerald, D.J., and Murray, F.E., Non-steroidal anti-inflammatory drugs (NSAIDs) and gastro-instinal toxicity :current issues, Ir. J. Med. Sci. 242-245 (1999)
  32. Siddhuraju, P. and Becker, K., Antioxidant properties of various solvent extracts oftotal phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera lam.) leaves. J. Agric food Chem. 51 (8), 2144-55 (2003) https://doi.org/10.1021/jf020444+
  33. Trinder, P., Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Ann. Clin. Biochem 6, 24 (1969)
  34. Winter, C.A., Risley, E.A., and Nuss, G.W., Carrageenan-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proc. Soc. Exp. Biol. Med. 111, 544 (1962)