Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effects of Resina Pini on the Growth and Glucosyltransferase activity of Streptococcus mutans

  • Seo, Young-A (College of Pharmacy, Catholic University of Daegu) ;
  • Choi, Nam-Ju (College of Pharmacy, Catholic University of Daegu) ;
  • Suk, Kui-Duk (College of Pharmacy, Catholic University of Daegu)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to evaluate the inhibitory effects of Resina Pini against Streptococcus mutans (S. mutans) that is one of the major causes of dental caries and oral diseases. Topically applied Resina Pini (RP) would be incorporated in saliva and thus the factor associated with water solubility should be considered. In this paper, therefore, effects of various treatment for RP and activities of water extracts from unprocessed and processed RP were compared. The crude RP (RP1) and the recrystallized RP (RP2) in ethanol solution showed strong antimicrobial activities (d.>15mm) against S. mutans. All RP samples exhibited considerable inhibitory effect against glucosyltransferase produced by S. mutans $(IC_{50}=91.2\;to\;276.2\;{\mu}g/ml)$. The very considerable increase in cellular permeability of S. mutans was observed with RP1, RP2 and their water extracts. These results suggest that RP1 and RP2 may be a potential source for pharmaceutical products used for prevention and/or treatment of dental caries and periodontal disease.

Keywords

References

  1. Bacca, L.A, Leusch, M., Lanzalaco, A.C., Macksood D., Bouwsma O.J., Shaffer J.B., Howard-Nordan K.S., Knippenberg S.H., Kreutzjans M.K., Miller lM., Poore C.L., Sunberg R.J., Vastola K.A Becus M., Bartizek R.D., Block R. P, Briner W.W, and White D.J., A comparison of intraoral antimicrobial effects of stabilized stannous fluoride dentifrice, baking soda/peroxide dentifrice, conventional NaP dentifrice and essential oil mouthrinse. J. Clin. Dent. 8, 54-61 (1997)
  2. Banas, J.A, Virulence properties of Streptococcus mutans. Front. Biosci. 9, 1267-77 (2004) https://doi.org/10.2741/1305
  3. Harvey, A, Strategies for discovering drugs from previously unexplored natural products. Drug Discov. Today, 5, 294-300 (2000) https://doi.org/10.1016/S1359-6446(00)01511-7
  4. Kwon, J.B., Lee, Y.W, An, B.J., and Lee, S.Y., Inhibitory effection of cacao bean husk extract on glucosyltransferase from Streptococcus mutans B13. Kor. J. Biotechnol. Bioeng. 8, 75-82 (1993)
  5. Kim, C.M., The pharmacognosy. The Korean pharmaceutical association, Seoul, 1995
  6. Goodson, J.M., Antimicrobial strategies for treatment of periodontal diseases. Periodontology 5, 142-168 (1994) https://doi.org/10.1111/j.1600-0757.1994.tb00022.x
  7. Lee, H.O., Han, D.M., and Baek, S.H., Isolation and identification of anticariotic compound from Sophora flavescens Ait. Kor. J. Microbiol. Biotechnol. 30, 420-424 (2002)
  8. Mombelli, A, Periodontitis as an infectious disease: specific features and their implications. Oral Dis. 9, 6-10 (2003) https://doi.org/10.1034/j.1601-0825.9.s1.2.x
  9. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests-Sixth Edition; Approved Standard. NCCLS Vol. 19 No.1 (1999)
  10. Namba, T., The encyclopedia of Wakan-Yaku, Vol. II, Hoikusa, Japan, 1980
  11. Nyvad, B., and kilian, M., Microbiology of the early colonization of human enamel and root surface in vivo, Scand. J. Dent. Res. 95, 369-380 (1987)
  12. Park, C.S., and Shin, Y.'S., Effect of Phellodendri cortex L. on the activity of glucosyltransferase and human gingival cell, growth and membrane permeability of Streptococcus mutans JC-2. J. Korean Acad. Dent. Health 19, 447-456 (1995)
  13. Park, W.J., Lee, H.J., and Yang, S.K., The inhibitory Effect of Sanggenon C from the Root-bark of Morus alba L. on the Growth and the Cellular Adherence of Streptococcus mutans. J. Korean pharm. 34, 434-438 (1990)
  14. Schwach-Abdellaoui, K., Loup, P.J, Vivien-Castioni, N., Mombelli, A., Baehni, P., Barr, L, Heller, L, and Gumy, R, Bioerodible injectable poly (ortho ester) for tetracycline controlled delivery to periodontal pockets: preliminary trial in humans. AAPS PharmSci. 4, 20 (2002)
  15. Song, H.K., and Kim, J.K; Essential oil components of leaves and resins from Pinus densiflora and Pinus koraiensis. Mokchae Konghak 22, 55-67 (1994)
  16. Tanzer, J,M., On changing the cariogenic chemistry of coronal plaque. J. Dent. Res. 68, 1576-1587 (1989)
  17. Trombelli, L., and Tatakis, D.N., Periodontal diseases: current and future indications for local antimicrobial therapy. Oral. Dis. 9, 11-5 (2003) https://doi.org/10.1034/j.1601-0825.9.s1.3.x
  18. Tsumori, H., and Kurarnitsu, H., The role of the Streptococcus mutans glucosyltransferases in the sucrose-dependent attachment to smooth surfaces: essential role of the GtfC enzyme. Oral Microbiol. Immunol. 12, 274-280 (1997) https://doi.org/10.1111/j.1399-302X.1997.tb00391.x
  19. Wu-Yuan, C.D., Gallotannins inhibit growth, water-soluble glucan synthesis and aggregation of Mutans streptococci. J. Dent. Res. 67, 51-55 (1988) https://doi.org/10.1177/00220345880670011001
  20. Yanagida, A., Kanda, T., Tanabe M., Matsudaira E, and Oliveira Cordeiro J.G., Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci. J. Agric. Food Chem. 48, 5666-71 (2000) https://doi.org/10.1021/jf000363i
  21. Yun, S.O., Fine tree and naturopathy, Academy press, Korea, 1997