The Effects Kunnuetang and Herbs on Mouse Neuroblastoma 2a Cells Damaged by Hypoxia-reoxygenation

건뇌양(健腦揚)과 단미(單味)들이 Hypoxia-Reoxygenation에 의해 손상받은 Mouse Neuroblastoma 2a Cells에 미치는 영향(影響)

  • Yeum, Chang-Sub (Dept. of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jong-Woo (Dept. of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Kang, Chul-Hun (Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Whang, Wei-Wan (Dept. of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University)
  • 염창섭 (경희대학교 한의과대학 신경정신의학교실) ;
  • 김종우 (경희대학교 한의과대학 신경정신의학교실) ;
  • 강철훈 (경희대학교 동서의학대학원) ;
  • 황의완 (경희대학교 한의과대학 신경정신의학교실)
  • Published : 2005.11.30

Abstract

Object : This study was designed to asses the effect of Kunnuetang and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by Kunnuetang, Duchang and Daejo. Result : 1. Kunnuetang, was effective on LDH assay of hypoxia and reoxygenation. 2. Duchang and Daejo were generally effective on LDH assay of hypoxia and reoxygenation. 3. In MTT assay of hypoxia Kunnuetang was not effective. Duchung and Daejo were not generally effective on MTT assay, but in certain condition Herbs were effective. 4. In MTT assay of reoxygenation Kunnuetang and Daejo were not effective. But Duchung was effective in certain condition. Conclusion : The results suggest that Kunnuetang, Duchang and Daejo may have protective effect on vascular dementia and ad patient.

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