The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Regulation of Vacuolar $H^+-ATPase$ c Gene Expression by Oxidative Stress

  • Kwak, Whan-Jong (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kim, Seong-Mook (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kim, Min-Sung (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kang, Jung-Hoon (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kim, Dong-Jin (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kim, Ho-Shik (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kown, Oh-Joo (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Kim, In-Kyung (Department of Biochemistry, College of Medicine, The Catholic University of Korea) ;
  • Jeong, Seong-Whan (Department of Biochemistry, College of Medicine, The Catholic University of Korea)
  • Published : 2005.10.21
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Abstract

By using differential display, we identified one of the genes encoding the multi-subunit complex protein V-ATPase, c subunit gene (ATP6L), and showed alterations of the gene expression by oxidative stresses. Expression of the ATP6L gene in Neuro-2A cells was increased by the treatment with $H_2O_2$ and incubation in hypoxic chamber, implying that the expression of the ATP6L gene is regulated by oxidative stresses. To examine mechanisms involved in the regulation of the gene expression by oxidative stresses, the transcriptional activity of the rat ATP6L promoter was studied. Transcription initiation site was determined by primer extension analysis and DNA sequencing, and promoter of the rat ATP6L and its deletion clones were constructed in reporter assay vector. Significant changes of the promoter activities in Neuro-2A cells were observed in two regions within the proximal 1 kbp promoter, and one containing a suppressor was in -195 to -220, which contains GC box that is activated by binding of Sp1 protein. The suppression of promoter activity was lost in mutants of the GC box. We confirmed by electrophoretic mobility shift and supershift assays that Sp1 protein specifically binds to the GC box. The promoter activity was not changed by the $H_2O_2$ treatment and incubation in hypoxic chamber, however, $H_2O_2$ increased the stability of ATP6L mRNA. These data suggest that the expression of the ATP6L gene by oxidative stresses is regulated at posttranscriptional level, whereas the GC box is important in basal activities of the promoter.

Keywords

References

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