The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 26 Issue 2
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- Pages.409-419
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- 2005
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- 1226-9174(pISSN)
Protective Effects of Boyanghwanoh-tang on Zinc-mediated Cytotoxicity in H9c2 Cardiomyoblast Cells
산화적 손상에 의해 유발된 심근세포 독성에 대한 보양환오탕(補陽還五湯)의 방어효과
- Rhim, Eun-Kyung (Wonkwang University Oriental Jeonju Medicine Hospital) ;
- Jeong, Hyun-Ae (Wonkwang University Oriental Jeonju Medicine Hospital) ;
- Shin, Sun-Ho (Wonkwang University Oriental Jeonju Medicine Hospital) ;
- Lee, Yun-Jae (Wonkwang University Oriental Jeonju Medicine Hospital)
- 임은경 (원광대학교 한의과대학 전주한방병원) ;
- 정현애 (원광대학교 한의과대학 익산한방병원) ;
- 신선호 (원광대학교 한의과대학 전주한방병원) ;
- 이윤재 (원광대학교 한의과대학 전주한방병원)
- Published : 2005.06.30
Abstract
The water extract of Boyanghwanoh-tang has been used for treatment of ischemic vascular disease in oriental medicine. However, little is known about the mechanism by which the water extract of Boyanghwanoh-tang rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of Boyanghwanoh-tang on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that, after treatment of H9c2 cells with zinc, there was a decrease in cell viability in a dose dependent manner, and there was a chromatin condensation. Zinc induced the change of cell morphology. In addition, zinc induced mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of Boyanghwanoh-tang consistently with increase of the peroxoredoxin 1, 2, 3, 5, and 6 expression. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells, and that protective effects of Boyanghwanoh-tang against oxidative injuries are achieved through regulation of peroxiredoin expression.