The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of HaengSoTang(HST), Gami-PalMiHwan(GPMH) on mucin secretion from airway goblet cells

행소탕(杏蘇湯) 및 가미팔미환(加味八味丸)이 호흡기(呼吸器) 배상세포(杯狀細胞)로부터의 뮤신 분비(分泌)에 미치는 영향

  • Lim, Do-Hee (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Lee, Joung-Eun (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Han, Young-Joo (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Hwang, Ji-Ho (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Cho, Cheol-Jun (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Bae, Han-Ho (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Chae, Eun-Young ;
  • Park, Yang-Chun (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
  • 임도희 (대전대학교 한의과대학 폐계내과학교실) ;
  • 이정은 (대전대학교 한의과대학 폐계내과학교실) ;
  • 한영주 (대전대학교 한의과대학 폐계내과학교실) ;
  • 황지호 (대전대학교 한의과대학 폐계내과학교실) ;
  • 조철준 (대전대학교 한의과대학 폐계내과학교실) ;
  • 배한호 (대전대학교 한의과대학 폐계내과학교실) ;
  • 채은영 (대전대학교 한의과대학 신계내과학교실) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Published : 2005.03.30
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Abstract

The intent of this study is to investigate whether two oriental medical prescriptions named haengsotang(HST) and gami-palmihwan(GPMH) significantly effect mucin release from cultured hamster tracheal surface epithelial(HTSE) cells, Confluent HTSE cells were metabolically radiolabeled with $^3H-glucosamine$ for 24 hrs and chased for 30 min in the presence of HST or GPMH to assess the effect of each agent on $^3H-mucin$ release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase(LDH) release. Also, the effects of HST and GPMH on contractility of isolated tracheal smooth muscle were investigated. The results are consistant with the following assertions: (1) HST significantly inhibited mucin release from cultured HTSE cells, without cytotoxicity; (2) GPMH did not effect mucin release without cytotoxicity; (3) HST and GPMH did not effect contractility of isolated tracheal smooth muscle. These results suggest a need for further investigation of HST and its components, for its potential in oriental medicine prescriptions and novel agents that effectively regulate (inhibit) mucin secretion from airway goblet cells.

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