Moutan Cortex Radicis contributes to the chemotaxis of eosinophils and secretion of cytokines in A549 human epithelial cells

목단피(牧丹皮)가 천식(喘息)유발 cytokine 분비와 호산구 chemotaxis에 미치는 영향

  • Moon, Sung-Hun (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Sung-Ki (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Rhee, Hyung-Koo (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Hee-Jae (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 문성훈 (경희대학교 한의과대학 폐계내과학교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학교실) ;
  • 이형구 (경희대학교 한의과대학 폐계내과학교실) ;
  • 정희재 (경희대학교 한의과대학 폐계내과학교실)
  • Published : 2005.03.30

Abstract

Objective : Airway inflammation is now regarded as a defining feature of asthma. The importance of eosinophits in the airway inflammation of asthma patients is widely recognized, and eosinophils mobilization in the respiratory epithelium is activated by chemoattractants and cytokines. This study was designed to examine the extent of the ability of Moutan Cortex Radicis to inhibit eosinophil chemotaxis of pulmonary epithelium after allergic stimulation. Material and Methods : Water extracts of Moutan Cortex Radicis and pulmonary epithelial cell lines A549(human type II-like epithelial cells) and human eosinophils were used. Cytotoxic effects of Moutan Cortex Radicis were estimated via MTS assay, and the effects of Moutan Cortex Radicis on chemokines from prestimulated A549 cells were estimated by sandwich ELISA and RT-PCR. Chemotaxis assay on prestimulated eosinophils treated with Moutan Cortex Radicis. was conducted Result : In this study we demonstrated that $TNF-{\alpha}$ and IL-4, $IL-1{\beta}$ induced the accumulation of chemokines' mRNA in the pulmonary epithelial cell lines A549 in a dose-dependent manner. Chemokines of eotaxin, ICAM-1, YCAM-1, IL-8, IL-16 were inhibited by Moutan Cortex Radicis in a dose dependent manner, but RANTES showed no inhibition due to Moutan Cortex Radicis. Eosinophil migration was inhibited at high concentrations of Moutan Cortex Radicis. Conculusion : These findings are indicative of supression of chemokines accomplished by Moutan Cortex Radicis treatment, demonstrating the potential therapeutic value of Moutan Cortex Radicis for treating diseases such as asthma.

Keywords