Journal of Acupuncture Research
- Volume 22 Issue 6
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- Pages.37-50
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
The Study of Anti-cancer Mechanism with Bee Venom and Melittin on Human Prostatic Cancer Cell
전립선 암세포에 대한 봉약침액(蜂藥浸液) 및 Melittin 약침액(藥浸液)의 항암(抗癌) 기전(機轉) 연구(硏究)
- Kim, Kyung-Tae (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Won University) ;
- Song, Ho-Sueb (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Won University)
- Published : 2005.12.20
Abstract
Objectives : The purpose of this study was to investigate the anti-caner effect of Bee Venom and Melittin on the prostatic cancer cell(PC-3). The goal of study is to ascertain whether Bee Venom and Melittin inhibits the cell growth and cell cycle of PC-3, or the expression of relative genes and whether the regression of PC-3 cell growth is due to cell death or the expression of gene related to apoptosis. Methods : After the treatment of Pc-3 cells with Bee Venom and Melittin, we performed Fluorescence microscope, MTT assay, Western blotting, Flow cytometry, PAGE electrophoresis and Surface plasmon resonance analysis to identify the cell viability, apoptosis and gene related to apoptosis. Results : 1. Compared with Control cell, the inhibition of cell growth reduced in proportion with the dose of Bee Venom or Melittin(