Journal of Acupuncture Research

  • 제22권5호
  • /
  • Pages.151-160
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  • 2005
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

호도약침액(胡桃藥浸液)이 t-Butylhydroperoxide에 의한 간손상(肝損傷)에 미치는 영향(影響)

The Effects of Juglandis Semen Herbal Acupuncture on t-Butylhydroperoxide-induced Liver Damage

  • 박상원 (동의대학교 한의과대학 침구경혈학교실) ;
  • 김철홍 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실)
  • Park, Sang-Won (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Kim, Cheol-Hong (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Youn, Hyoun-Min (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Jang, Kyung-Jeon (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Ahn, Chang-Beohm (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Song, Choon-Ho (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University)
  • 발행 : 2005.10.20
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초록

Objectives : This study was undertaken to examine whether Juglandis Semen herbal acupuncture (JGA) exerts protective effect against oxidant-induced cell injury in rabbit liver. Methods : The cell damage was estimated by measuring lactate dehydrogenase (LDH) release, and lipid peroxidation was estimated by measuring malondialdehyde (MDA) in rabbit liver slices. Results : t-Butylhydroperoxide (tBHP) caused an increase in LDH release and lipid peroxidation in a dose-dependent manner over concentrations of 0.5-2 mM, which were prevented by addition of 0.05% JGA. The protective effect of JGA was dose-dependent in concentration range of 0.005 to 0.1%. The concentrations of 0.005 and 0.1% JGA completely prevented the LDH release and lipid peroxidation by 1 mM tBHP. When liver tissues were exposed to 1 mM tBHP, alanine aminotransferase (ALT) activity in the medium was significantly increased, which was prevented by 0.05% JGA. tBHP (2 mM) decreased GSH content and the effect was prevented by 0.05% JGA. Conclusion : These results suggest that JGA exerts protective effect against oxidant-induced cell injury by antioxidant action resulting from enhancement of GSH content in the liver.

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