The Korea Journal of Herbology (대한본초학회지)
- Volume 20 Issue 4
- /
- Pages.151-161
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- 2005
- /
- 1229-1765(pISSN)
- /
- 2288-7199(eISSN)
The Relative Identification of C. officinale and L. chuanxiong by PCR-Mediated Fingerprinting
천궁류(川芎類) 한약재의 유전자 감식 연구
-
Choi, Ho-Young
(College of Oriental Medicine, Kyung Hee University)
;
- Kim, Dong-Wook (College of Oriental Medicine, Kyung Hee University) ;
- Kim, Dong-Eun (College of Oriental Medicine, Kyung Hee University) ;
-
Suh, Young-Bae
(NPRI, Seoul National University)
;
-
Ham, In-Hye
(College of Oriental Medicine, Kyung Hee University)
-
최호영
(경희대학교 한의과대학)
;
- 김동욱 (경희대학교 한의과대학) ;
- 김동은 (경희대학교 한의과대학) ;
-
서영배
(서울대학교 약학대학 천연물과학연구소)
;
-
함인혜
(경희대학교 한의과대학)
- Published : 2005.12.30
Abstract
Objectives : Our research purpose is to establish the standard identification analysis on C. officinale and L. chuanxiong in Korea and China by PCR-mediated fingerprinting. Methods : The Restriction Fragment Length Polymorphism (RFLP) and Randomly Amplified Polymorphic DNA (RAPD) method was used on Internal Transcribed Spacer (ITS) regions and rbcL regions to compare and discriminate genes extracted from crude drugs as C. officinale and L. chuanxiong in Korea and China. Results : L. chuanxiong Korea and China have very similar polymorphism, whereas L. chuanxiong in Korea and C. officinale have very different polymorphism in RFLP. And restriction enzymes AluI and SacI forms the specific fragment band only in C. officinale, they can be used as RFLP marker on ITS regions to discriminate among the species. Conclusions : The results could be applied in discriminating crude drugs among C. officinale and L. chuanxiong in Korea and China. Also they could be used in controlling drug quality, preserving medicinal plants, and improving plant description.
Keywords