대한본초학회지 (The Korea Journal of Herbology)
- 제20권2호
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- Pages.55-60
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- 2005
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
Raw 264.7 cell에서 차가 버섯 에탄올 추출물이 Cytokine 발현에 미치는 영향
Effects of Inonotus obliquus Ethanol Extract on Cytokine Expression in Raw 264.7 Cell
초록
Objectives : This study was conducted to investigate the effects Innotus obliquus on the cytokine expression in Raw 264.7 cells. Methods : In Raw 264.7 cells stimulated with lipopolysaccaride(LPS) to Mimic inflammation, Innotus obliquus ethanol extract inhibited nitric oxide production in a dose-dependent manner and abrogated inducible nitric oxide sythase(iNOS) and cyclooxigenase (COX-2). The levels of cytokines were analyzed by sandwich immuno assays. Results and Conclusions : Results provided evidence that Innotus obliquus inhibited the production of IL-b, and IL-6 in Raw 264.7 cells activated with lipopolysaccharide (LPS). These finding suggested that Innotus obliquus can produce anti-inflammatory effect, which may playa role in adjunctive therapy in Gram-negative bacterial infections.