동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제19권3호
  • /
  • Pages.705-709
  • /
  • 2005
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

율무가 백혈병세포주인 L1210 세포의 증식에 미치는 영향

Effect of the Seeds of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the Proliferation of L1210 cells, a Leukemic cell-lines

  • 발행 : 2005.06.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this research was to investigate the effects of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the proliferation of L1210 cells and immune cells. The hexane fraction of Coix Lachryma-Jobi decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. Also, the fraction decreased the cell viability of murine thymocytes and splenocytes, but increased the phagocytic activity of murine peritoneal macrophages. In addition, fatty acids and fatty acid methyl esters decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. The main components of the fraction are fatty acid and their derivatives. These results indicate that Coix Lachryma-Jobi has a cytotoxicity on tumor cells and immune cells.

키워드

참고문헌

  1. 生藥學研究會 著. 現代生藥學, 學窓社, pp 529-530, 1998.
  2. 藥品植物學研究會 著:新.藥品植物學, 學窓社, pp 216-217, 1998.
  3. Zhang, Y., Hou, G. and Yue, Y. The analgesic action of semen coicis on severe functional dysmenorrhea-a sequential trial observation. J. Tradit. Chin. Med., 20(4):293-296, 2000.
  4. Hidaka, Y., Kaneda, T., Amino, N., Miyai, K Chinese medicine, Coix seeds increase peripheral cytotoxic T and NK cells. Biotherapy, 5(3):201-203, 1992.
  5. Kaneda, T., Hidaka, Y., Kashiwai, T., Tada, H., Takano, T., Nishiyama, S., Amino, N. and Miyai, K Effect of coix seed on the changes in peripheral lymphocyte subsets. Rinsho Byori, 40(2):179-181, 1992.
  6. Ukita, T., Tanimura, A. Studies on the anti-tumor component in the seeds of Coix Lachryma-Jobi L. var. Mayuen (Roman.) Stapf. I. Chem. Pharm. Bull. 9(1):43-46, 1961.
  7. Tanimura, A. Studies on anti-tumor component in the seeds of Coix Lachryma-jobi L. var. Ma-yuen (Roman.) Stapf. II. Chem. Pharm. Bull. 9(1):47-53, 1961.
  8. Numata, M., Yamamoto, A., Moribayashi, A., Yamada, H. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jobi. Planta Med. 60(4):356-359, 1994.
  9. Tokuda, H., Matsumoto, T., Konoshima, T., Kozuka, M., Nishino, H., Iwashima, A Inhibitory effects on Epstein-Barr virus activation and anti-tumor promoting activities of Coix Seed. Planta Med. 56, 653, 1990.
  10. Numata, M., Yamamoto, A., Moribayashi, A., Yamada, H. Antitumor components isolated from the chinese herbal medicine Coix lacjryma-jobi. Planta Med, 60, 356-359, 1994.
  11. Kim, S.J.: Antimutagenic and anticancer effects of Coix laqrymajobi(adlay) and identification of the active components. Graduate School, Pusan National University. 1999.
  12. Hayatsu, H., Arimoto, S., Negishi, T. Dietry inhibitors of mutagenesis and carcinogenesis. Mutation Res., 202, 429, 1988.
  13. Mosmann, T. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxic assays. J. Immunol. methods. 65, 55-61, 1983.
  14. Kotnic, V., Fleischmann, W.R.Jr. A simple and rapid method to determine hematopoietic growth factor activity. J. Immunol. methods. 129, 23-28, 1990.
  15. Nicoletti, I., Migliorati, G., Pagliacci, M.C, Grignani, F., Riccardi, CA. Rapid and simple method for mesuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods. 139, 271-279, 1991.
  16. Wysocki, L.J., Sato, V.L. Planning for lymphocytes: A method for cell selection. Proc. Natl. Acad. Sci. USA, 75, 2844-2848, 1978.
  17. Mizel, S.B., Openheim, J.J., Rosensteich, D.L Characterization of lymphocyte-activatingfactor{LAF) produced by the macrophage cell line P388D1. J. Immunol., 120, 1497-1503, 1979.
  18. Channon, J.Y., Leslie, CC, Johnston, Jr.R.B. Zymosanstimulated production of phosphatidic acid by macrophages: relationship to release of superoxide anion and inhibition by agents that increase intracellular cyclic AMP. J. Leucocyte Biol., 41, 450-455, 1987.
  19. Breiheim, G., Stendahl, O., Dahlgren, C. Intra-and extracellular evevts in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Infect. Immun., 45, 1-8, 1984.
  20. 김진순. 율무지방질의 산화안전성 및 관련 항산화물질에 관한 연구. 부산대학교 대학원 석사학위논문, 1995.
  21. Ohtsubo, K., Yanase, H., Hashimoto, K. Studies on the storage of Job's tears. Part I. Changes in Job's tears quality during storage under natural condition and storage at 40$^{\circ}C$. Rept. Nat'l. Food Res. Inst., 45, 76, 1984.
  22. 김기환. 의이인 Ethanol extract의 혈압강하작용. 조선대학교, 제약연구, 41, 1980.
  23. 박양자, 이영선, 銀木平光. 율무쌀이 쥐의 혈장 콜레스테롤 및 지질대사에 미치는 영향. 한국영양학회지, 21(2):88, 1988.
  24. 石黑幸雄, 岡本質治, 圓田洋沈 : Antimicrobial activity in Etiolated Seedings of Adlay. 日本食品工業學會誌, 40(5):353, 1993.
  25. Takahashi, M., Konno, C, Hikino, H. Isolation and hypoglycemic activity of Coixans A, Band C, glycans of Coix lachryma-jobi var. ma-yuen seeds. Planta Med. 52, 64, 1986.
  26. Mitsuhiro, N., Akihiko, Y., Atsuko, M., Hiromi, Y. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jobi., Planta Med. 60, 356, 1994.
  27. Chung, S. Park, S., Yang, C.H. Unsaturated fatty acids bind Myc-Max transcription factor and inhibit Myc-Max-DNA complex formation. Cancer Latt., 188(1-2):153-162, 2002.
  28. Chen, Q., Galleano, M.,Cederbaum, A.I. Cytoxicity and apoptosis produced by arachidonic acid in HepG2 cells overexpressing human cytochrome P-4502E1. Alcohol Clin. Exp. Res., 22(4):782-782, 1998.