동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제19권3호
  • /
  • Pages.656-661
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  • 2005
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Methoxychlor투여 흰쥐에 있어서 복분자 술이 Testosterone에 미치는 효과

Effect of Black Raspberry Wine to Testosterone in Sprague-Dawley Rats Administrated with Methoxychlor

  • 발행 : 2005.06.25
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초록

The study was conducted to investigate per oral (PO) effects of Black raspberry wine on testosterone levels in Sprague-Dawley rats oral administrated with Methoxychlor in order to establish the experimental clinical model for evaluating the influences on the sexual hormones of SD-Rat administrated with Methoxychlor(MET), it was dissolved in acetone and olive oil (1:19), which was administrated orally at doses of 200mg/kg body weight/ day for 7days. Black raspberry wine of 13% alcohol concentration, was prepared from ripen fruits of Rubus coreanus fermented with Saccharomyces cervisiae. PO administration of Black raspberry wine for 15 week produced dramatic increases of serum testosterone levels. Increase in the testosterone level was observed, using gamma counter with 125I testosterone, starting from 1 week post administration. Maximum increase in testosterone level was observed at 4 week post administration, 5.18${\pm}$0.76ng/mL, which was 10.1 times higher than before and at 15 weeks post administration it was recorded as 1.67${\pm}$0.19ng/mL indicating Black raspberry wine as an effective phyto-testosteronic beverage of the future.

키워드

참고문헌

  1. 허준,동의보감,pp 603, 711, 남선당,1977.
  2. 사관. 동양의학대사, P 1098, 고문사,1975.
  3. 윤진표. 복분자 전액 투여에 의한 가토의 자궁 및 난포 발육 촉진에 관한 연구. 원광대학교 대학원 한의학과 학위논문집. 443-465. 1982.
  4. Chen, K, Fang, J., Kuang, X., Mo. Q. Effects of the fruit of Rubus chingii Hu on hypothalamus - pituitary - sex gland axis in rats. Zhongguo Zhong Yao Za Zhi. 21(9):560-562, 1996.
  5. Ono, M., Tateishi, M., Masuoka, C, Kobayashi, H., Igoshi, K, Komatsu, H., Ito, Y., Okawa, M., Nohara, T. A new triterpene glucosyl ester from the fruit of the blackberry(Rubus allegheniensis). Chem. Pharm. Bull, 51(2):200-202, 2003.
  6. Lee, J.H., Ham, Y.A., Choi, S.H., Im, E.O., Jung, J.H., Im, K.S., Kim, D.K., Xu, Y., Wang, M.W., Kim, N.D. Activity of crude extract of Rubus crataegifolius roots as a potent apoptosis inducer and DNA topoisomerase I inhibitor. Arch. Pharm. Res. 23(4):338-343, 2000.
  7. Marquina, M.A., Corao, G.M., Araujo, L., Buitrago, D., Sosa, M. Hyaluronidase inhibitory activity from the polyphenols in the fruit of blackberry(Rubus fruticosus B.). Fitoterapia. 73, 727-729. 2002.
  8. Shin, T.Y., Kim, S.H, Lee, E.S., Eom, D.O., Kim, H.M. Action of Rubus coreanus extract on systemic and local anaphylaxis. Phytother Res. 16, 508-513, 2002.
  9. Hassan, J., Mhamed, M., Mohamed, E., Hypoglycaemic effect of Rubus fructicosis L. and Globularia alypum L. in normal and streptozotocin-induced diabetic rats. Journal of Ethnopharmacology. 81, 351-356, 2002.
  10. 차한수, 박민선, 박기문. 복분자 딸기의 생리활성. 한국식품과 학회지. 33(4):409-415, 2001.
  11. Thiem, B., Coslinska, O. Antimicrobial activity of Rubus chamaemorus leaves. Fitoterapia. 75, 93-95, 2004.
  12. Alanis, A.D., Calzada, F., Cedillo-Rivera, R., Meckes, M. Antiprotozoal activity of the constituents of Rubus coriifolius. Phytotherapy Research. 17, 681-682, 2003.
  13. Kim, T.G., Kang, S.Y., Jung, K.K., Kang, J.H., Lee, E., Han, H.M., Kim, S.H. Antiviral activities of extracts isolated from Terminalis chebula Retz., Sanguisorba officinalis L., Rubus coreanus Miq. and Rheum palmatum L. against hepatitis B virus. Phytother Research. 15, 718-720, 2001.
  14. 백병걸, 임채웅, 이은영, 황인수, 권혁년, 이희곤, 이성일, 이형자, 전병훈. 복분자 술이 흰쥐의 testosterone 분비에 미치는 영향 동의생리병리학회지. 18(4):1007-1013, 2004.
  15. Facemire, C.F., Gross, T.S., Guillette, L.J., Jr. Reproductive impairment in the Florida panther: nature or nurture? Environ. Health Perspect. 4, 79-86, 1995.
  16. Heindel, J.J. Endocrine disruptors and obesity epidemic. Toxicological Sciences, 76, 247-249, 2003.
  17. Leavens, T.L., Sparrow, B.R, Devito, M.J. Lack of antiandrogenic effects in adult male rats following acute exposure to 2,2-bis(4-chlorophenyl)-1,1-dichloroethvlenc (p,p -DDE). Toxicology, 174, 69-78, 2002.
  18. Smith, E., Andrew, G. Chlorinated Hydrocarbon Insecticides. in Handbook of Pesticide Toxicology, Volume 3, Classes of Pesticides. Wayland J. Hayes Jr. and Edward R. Lows, Jr. editors. Academic Press, Inc., NY. 1991.
  19. Stresser, D.M., Kupfer, D. Human cytochrome P450catalyzed conversion of the proestrogenic pesticide methoxychlor into an estrogen. Role of CYP2C19 and CYPlA2 in O-Demethylation. Drug Metabolism and disposition, 26(9):868-874, 1998.
  20. Chapin, R.E., Harris, M.W., Davis, B.J., Ward, S.M., Wilson, R.E., Mauney, M.A., Lockhart, A.C., Smialowiez, R.J., Moser, V.C., Burka, L.T., Collins, B.J. The effects of perinatal/juvenile methoxychlor exposure on adult rat nervous, immune, and reproductive system function. Fundum Appl, Toxicol., 40, 138-157, 1997.
  21. Sharpe, R.M., Skakkebaek, N.E. Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? Lancet. 341, 1392-1395, 1993.
  22. Latchoumycandane, C., Chitta, K.C, Mathur, P.P. The effect of methoxychlor on the epididymal antioxidant system of adult rats. Reproductive Toxicology. 16, 161-172, 2002.
  23. 백병걸, 이부웅, 임채웅, 권혁년, 허진, 이은영, 이희곤, 이성일, 김병수, 이형자, 전병훈. Methoxychlor 투여한 흰쥐에 있어서의 testosterone의 변화 동의생리병리학회지 18(4):1163-1168, 2004.
  24. Ismail, A.A., Astley, P., Burr, W.A., Cawood, M., Short, F., Wakelin, K, Wheeler, M.J. The role of testosterone measurement in the investigation of androgen disorders. Ann. C1in. Biochem. 23(Pt2):113-134, 1986.
  25. Reuber, M.D. Carcinogenicity and toxicity of methoxychlor. Environ Health Perspect, 36, 205-219, 1980.
  26. Chitra, K.C., Latchoumycandane, C., Mathur, P.P. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. Toxicology, 185, 119-127, 2003.
  27. Masutomi, N., Shibutani, M., Takagi, H., Unryama. C., Takahashi, N., Hirose, M. Impact of dietary exposure to methoxychlor, genistein, or diisonoyl phthalate during the perinatal period on the development of the rat endocrine/reproductive systems in later life. Toxicology, 192, 149-170, 2003.
  28. Ohsako, S., Miyabara., Y., Sakaue, M., Ishimura, R, Kakeyama, M., Izumi, H., Yonemoto, J., Tohyarna, C. Developmental stage - specific effects of perinatal 2,3,7,8,-tetrachlorodibenzo -p-dioxin exposure on reproductive organs of male rat offspring. Toxicological Science, 66, 283-292, 2002.
  29. Kunimatsu, T., Yamada, T., Ose, K., Sunami, O., Kamita, Y., Okuno, Y., Seki, T., Nakatsuka, I. Lack of (anti-) androgenic or estrogenic effects of three pyrethroids(Esfenvalerate, Fenvalerate, and Permethrin) in the Hershberger and uterotrophic assays. Regulatory Toxicology and Pharmacology, 35, 227-237, 2002.
  30. Wistuba, J., Brinkworth, M.H., Schlatt, S., Chahoud, I., Nieschlag, E. Intrauterine bisphenol A exposure leads to stimulatory effects on Sertoli cell number in rats. Environ mental Research. 91, 95-103, 2003.
  31. Gray, L.E., Jr. KeIce, W.R., Monosson, E., Ostby, J.S. Birnbaum LS. Exposure to TCDD during development permanently alters reproductive function in male Long Evans rats and hamsters. Reduced ejaculated and epididymal sperm numbers and sex accessory gland weights in offspring with normal androgenic status. Toxicology and applied Pharmacology, 131, 108-118, 1995.
  32. Wang, B.G., Zhu, W.M., Li, X.M., Jia, Z.J., Hao, X.J. Rubupungenosides A and B, two novel triterpenoid saponin dimers from the aerial parts of Rubus pungens. J. Nat. Prod. 63, 851-854, 2000.
  33. Mullen, W., Yokota, T., Lean, M.E., Crozier, A. Analysis of ellagitannins and conjugates of ellagic acid and quercetin in raspberry fruits by LC-MSn. Phytochemistry. 64, 617-624. 2003.
  34. 강소신의학원편. 중약대사전,pp 2698-2699, 1977.
  35. 이상점, 현대한방약물학. pp 385, 행림서원, 1974.
  36. Carlsen, E. Giwercman, A., Keiding, N., Skakkebaek, N.E. Evidence for decreasing quality of semen during past 50 years. B. M. J. 12, 609-613, 1992.
  37. You, L., Casanova, M., Archibeque-Engle, S., Sar, M., Fan, L.Q., Heck, H.A. Impaired male sexual development in perinatal Sprague-Dawley and Long-Evans hooded rats exposed in utero and lactationally to p,p’DOE. Tox. Sci., 45, 162-173, 1998.
  38. Blizard, D., Sueyoshi, T., Negishi, M., Dehal, S.S., Kupper , D. Mechanism of induction of cytochrome P450 enzymes by the proestrogenic endocrine disruptor pesticideMethoxychlor: Interactions of Methoxychlor metabolites with the constitutive androstane receptor system. Drug Metabolism and disposition. 29, 781-785, 2001.
  39. 김지영. 의부전서, pp. 9284, 9289, 가국중앙도서관장판, 금영출판사,1975.
  40. Fox, J.G., Cohen, B.J., Loew, F.M. Laboratory animal medicine. Academic Press. INC. 121-1349, 2002.