A Study on the Protective Mechanism of Moxi-tar on Cytotoxicity Induced by $H_2O_2$ in $C_6-glioma$

구진의 $H_2O_2$로 유발된 뇌신경세포 상해에 대한 보호 기전 연구

  • Ahn, Seoung-Hoon (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University) ;
  • Ahn, Young-Nam (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University) ;
  • Baek, Dae-Bong (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University) ;
  • Song, Moon-Young (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Kyoung-Sik (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University) ;
  • Sohn, In-Cheul (Dept. of Acupoint & Meridian, College of Oriental Medicine, Wonkwang University)
  • 안성훈 (원광대학교 한의과대학 경혈학교실) ;
  • 안영남 (원광대학교 한의과대학 경혈학교실) ;
  • 백대봉 (원광대학교 한의과대학 경혈학교실) ;
  • 송문영 (원광대학교 한의과대학 경혈학교실) ;
  • 김경식 (원광대학교 한의과대학 경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실)
  • Published : 2005.06.27

Abstract

Objective : This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased NO and iNOS generation in $C_6-glioma$ and RAW 264.7 cells in our lab. The purpose of this research was to investigate the protect reaction on cell injury induced by the $H_2O_2$ in $C_6-glioma$ cells. Methods : $C_6-glioma$ cells were cultured in RPMI 1640 with FBS 10% in $CO_2$ incubator. To study the protective effects of moxi-tar, we observed cell viability, DPPH activity, SOD activity, catalase activity and cell morphology after injury with $H_2O_2$. Results : Moxi-tar increased cell viability about twice as much as that of being injury by $H_2O_2$(moxi-tar $40\;{\mu}g/m{\ell}$, $H_2O_2\;500\;{\mu}\;M$). And the results of free radical scavenger activity ($80\;{\mu}g/\;m{\ell}\;:\;78.91\;{\pm}\; 4.4%$), SOD activity and catalase activity ($80\;{\mu}g/\;m{\ell}$: 21.6 unit/ mg protein) were increased by moxi- tar as dose-dependent manner. Conclusion: we concluded that the effects of moxibustion which is played important role in Oriental medicine, might be free radical scavenger effects induced by moxi-tar.

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