Comparative Efficacy of Mosquito Repellents against Aedes albopictus (Diptera: Culicidae)

흰줄숲모기에 대한 모기기피제의 효과비교

  • Kang, Shin-Ho (Department of Plant Medicine, Chungbuk National University) ;
  • Jang, Sun-Ah (Department of Plant Medicine, Chungbuk National University) ;
  • Han, Jong-Been (Department of Plant Medicine, Chungbuk National University) ;
  • Seo, Dong-Kyu (Department of Plant Medicine, Chungbuk National University) ;
  • Song, Chi-Hun (Department of Plant Medicine, Chungbuk National University) ;
  • Kim, Min-Ki (Department of Plant Medicine, Chungbuk National University) ;
  • Kim, Young-Lim (Drug Evaluation Department, Korea Food & Drug Administration) ;
  • Choi, Seon-Hee (Drug Evaluation Department, Korea Food & Drug Administration) ;
  • Kim, In-Kyu (Drug Evaluation Department, Korea Food & Drug Administration) ;
  • Kim, Gil-Hah (Department of Plant Medicine, Chungbuk National University)
  • 강신호 (충북대학교 농업생명환경대학 식물의학과) ;
  • 장선아 (충북대학교 농업생명환경대학 식물의학과) ;
  • 한종빈 (충북대학교 농업생명환경대학 식물의학과) ;
  • 서동규 (충북대학교 농업생명환경대학 식물의학과) ;
  • 송치훈 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김민기 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김영림 (식품의약품안전청 의약품평가부) ;
  • 최선희 (식품의약품안전청 의약품평가부) ;
  • 김인규 (식품의약품안전청 의약품평가부) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2005.09.01

Abstract

We investigated the repellency of eight mosquito repellents against Culex pipiens pallens and Aedes albopictus, and the quantity of DEETs were analysed by using gas chromatography. Ae. albopictus showed higher bloodsucking efficacy than Cx. pipiens pallens of the two subjects mouse and human hands, and human hand was more efficacious method for mosquito bloodsucking efficacy than mouse. Among the eight repellents were tested with mouse as subjects for eight hours, product C, G, and H were high repellency than others. In the case of human hands as subject, product C was highly effective than above other products. The DEET quantities of eight repellants varied from 7.31 g to 38.9 g in 100 g contents and we ascertained that there was a correlation between mosquito repellency and the DEET quantities remained after the treatments. It was evidenced from the long term test with shirt piece, in this test the Ae. albopictus attack was increased when the DEET quantity decreased below 40%.

모기기피제로 사용되고 있는 에어로졸제 4개, 액제 3개, 그리고 로션제 1개 제품에 대하여 흰줄숲모기(Aedes albopictus)와 빨간집모기(Culex pipiens pallens)의 기피력을 조사하고, 기피 주성분인 DEET의 함량을 분석하였다. 쥐를 대상으로 했을 때 흰줄숲모기는 빨간집모기에 비해 흡혈 공격력이 높았으며, 흡혈 대상으로는 쥐(mouse)보다 사람의 손을 더 선호하였다. 쥐를 이용한 모기기피제의 기피력 평가에서, C 제품, G 제품, H 제품이 높은 기피력을 나타냈다. 이들 기피제를 사람 손을 흡혈대상으로 평가한 결과, 3개 약제 모두 2시간 동안은 100%의 기피효과를 나타내었으나 그 이후는 떨어줬으며, C 제품이 효과가 좋았다. GC를 통한 DEET의 분석에서 8종 모기기피제의 내용물 100 g 중량 내 7.31 g에서 38.9 g으로 제품 내 다양한 함량을 나타내었으며, 제품 내 처리된 DEET의 함량과 기피력의 상관관계는 정비례하지 않았다. 면포를 이용한 DEET 잔존량 대비 모기 기피력 평가에서 DEET의 함량이 약 40% 이하로 떨어지면서 흰줄숲모기의 흡혈이 이루어지기 시작했다.

Keywords

References

  1. Badolo, A., E. Ilboudo-Sanogo, A.P. Ouedraogo and C. Costantini. 2004. Evaluation of the sensitivity of Aedes aegypti and Anopheles gambiae complex mosquitoes to two insect repellents: DEET and KBR 3023. Tropical Medicine and International Health. 9: 330-334 https://doi.org/10.1111/j.1365-3156.2004.01206.x
  2. Barnard, D.R. and R-D. Xue. 2004. Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochlerotatus triseriatus (Diptera: Culicidae) J. Med. Entomol. 41: 726-730 https://doi.org/10.1603/0022-2585-41.4.726
  3. Costantini, C., A. Badolo and E. Ilboudo-Sanogo. 2004. Field evaluation of the efficacy and persistence of insect repellents DEET, IR3535 and KBR 3023 against Anopheles gambiae complex and other afrotropical vector mosquitoes. Trans. R. Soc. Trop. Med. Hyg. 98: 644-652 https://doi.org/10.1016/j.trstmh.2003.12.015
  4. Debboun, M. and J. Wagman. 2004. In vitro repellency of N,N-diethyl-3-methylbenzamide and N,N-diethylphenylacetamide analogs against Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). J. Med. Entomol. 41: 430-434 https://doi.org/10.1603/0022-2585-41.3.430
  5. Frances, S.P. and R.D. Cooper. 2002. Personal protection measures against mosquitoes. ADF Health. 3: 58 -63
  6. Frances, S.P., D.G.E. Waterson, N.W. Beebe and R.D. Cooper. 2004. Field evaluation of repellent formulations containing deet and picaridin against mosquitoes in northern territory, Australia. J. Med. Entomol. 41: 414-417 https://doi.org/10.1603/0022-2585-41.3.414
  7. Frances S.P., R.D. Cooper and A.W. Sweeney. 1998. Laboratory and field evaluation of the repellents deet, CIC-4 and A13-37220 against Anopheles farauti (Diptera: Culicidae) in Australia. J. Med. Entomol. 35: 690-693 https://doi.org/10.1093/jmedent/35.5.690
  8. Kim, S.I., K.S. Chang, Y.C. Yang, B.S. Kim and Y.J. Ahn. 2004. Repellency of aerosol and cream products containing fennel oil to mosquitoes under laboratory and field conditions. Pest Manag. Sci. 60: 1125 -1130 https://doi.org/10.1002/ps.921
  9. Klun J.A. and M. Debboun. 2000. A new module for quantitative evaluation of repellent efficacy using human subjects. J. Med. Entomol. 37: 177-181 https://doi.org/10.1603/0022-2585-37.1.177
  10. Koren, G., D. Matsui and B. Bailey. 2003. DEET-based insect repellents: safety implications for children and pregnant and lactating women. CMAJ. 169: 209-212
  11. Lee, W.J., H.S. Lee, Y.J. Ahn and D.K. Lee. 2004. Laboratory evaluation of controlled-release repellent treated pulp fabric on human volunteers against mosquito vectors. Entomological Research. 34: 37-42 https://doi.org/10.1111/j.1748-5967.2004.tb00088.x
  12. Mark, S., M.D. Fradin and F.D. John. 2002. Comparative efficacy of insect repellents against mosquito bites. N. Engl. J. Med. 347: 13 -18 https://doi.org/10.1056/NEJMoa011699
  13. Personal insect repellents containing DEET(N,N-diethyl-m-toluamide and related compounds) [Re-evaluation decision document RRD2002-01]. Ottawa: Pest Management Regulatory Agency. Health Canada; 2002. Apr. 15. Available:
  14. Peterson, C. and J. Coats. 2001. Pesticide outlook-August 2001. 154-158
  15. Qiu, H., H. Won Jun and J. Tao. 1997. Phamacokinetics of insect repellent N,N-diethyl-m-toluamide in Beagle Dogs following intravenous and topical routes of administration. J. Pharmac. Sci. April. 514-516
  16. Rozendaal, J.A. 1997. Vector control. World Health Organization, Geneva, Switzerland
  17. Rueda, L.M., L.C. Rutledge and R.K. Gupta. 1998. Effect of skin abrasion on the effecacy of the repellent deet against Aedes aegypti. Journal of the Am. Mosq. Control Association. 14: 178-182
  18. Sadanandane, C., N. Mathew, P. Jambulingam and M. Kalyanasundaram. 2001. Laboratory & field evaluation of controlled release formulation of the insect repellents N,N-diethyl-mtoluamide (DEET) and N,N-diethylphenylacetamide (DEPA) against mosquito vectors. Indian J. Med. Res. 113: 108-112
  19. Safety tips on using personal insect repellent. 2003. Ottawa: Pest Management Regulatory Agency, Health Canada; Available: (accessed 2003 July 7)
  20. Tawatsin, A., S.D. Wratten, R Scott, D. Thavara and Y. Techadamrongsin. 2001. Repellency of volatile oils from plants three mosquito vectors. Journal of Vector Ecology. 26: 76-82
  21. Thavara, D., A. Tawatsin, J. Chompoosri, W. Suwonkerd, D.R. Chansang and P. Asavadachanukorn. 2001. Laboratory and field evaluations of the insect repellent 3535 (ethylbutylaminopropionate) and deet against mosquito vectors in Thiland. J. Am. Mosq. Control Assoc. 17: 190-195