Journal of Institute of Control, Robotics and Systems (제어로봇시스템학회논문지)

Institute of Control, Robotics and Systems (제어로봇시스템학회)
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Study of in Silico Simulation Method for Dynamic Network Model in Lactic Acid Bacteria

Lactic Acid Bacteria의 동역학 네트워크 모델을 이용한 in Silico 모사방법 연구

  • 정의섭 (서강대학교 화공생명공학과) ;
  • 이혜원 (서강대학교 화공생명공학과) ;
  • 이진원 (서강대학교 화공생명공학과)
  • Published : 2005.10.01
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Abstract

We have newly constructed an in silico model of fermentative metabolism for Lactococcus lactis in order to analyze the characteristics of metabolite flux for dynamic network. A rigorous mathematical model for metabolic flux has been developed and simulation researches have been performed by using GEPASI program. In this simulation task, we were able to predict the whole flux distribution trend for lactate metabolism and analyze the flux ratio on the pyruvate branch point by using metabolic flux analysis(MFA). And we have studied flux control coefficients of key reaction steps in the model by using metabolic control analysis(MCA). The role of pyruvate branch seems to be essential for the secretion of lactate and other organic byproducts. Then we have made an effort to elucidate its metabolic regulation characteristics and key reaction steps, and find an optimal condition for the production of lactate.

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