Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Haematological Parameters Induced by Benzo(a)pyrene Exposure as a Toxicity Biomarker in the Fanned Red Sea Bream, Pagrus major

  • Choy, Eun-Jung (Department of Biology, Pusan National University) ;
  • Jo, Qtae (Aquaculture Division, East Sea fisheries Research Institute) ;
  • Kang, Chang-Keun (Department of Biology, Pusan National University)
  • Published : 2005.08.25
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Abstract

Farmed red sea breams, Pagrus major, were fed for 60 days with pellets containing different concentrations of benzo(a)pyrene (0, 0.2, 2, 20 mg/kg) to generate a biomarker of the chemical toxicity in the fish. The fish exposed to the chemical concentrations did not show any significant difference in the weight gain, conditioning, factor, and hepatosomatic index. However, some haematological parameters, such as glucose, calcium, magnesium, GOT (glutamic oxalate transaminase), and GPT (glutamic pyruvate transaminase) were influenced by the chemical exposure. Of them, two enzymes, GOT and GPT, increased significantly 60 days after the exposure in a way of concentration dependence (P<0.05). In the study of ecotoxicological biomarker, sensitivity to adverse environments is one of the key available factors. The fish changes in GOT and GPT were an earlier and reliable sign of the fish response against the chemical exposure, rendering the two enzymatic factors as a useful biomarker at least to benzo(a)pyrene exposure in the farming waters.

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References

  1. Blaxhall, P. C., 1972. The haematological assessment of the health of freshwater fish: a review of selected literature. J. Fish BioI., 4,593-604 https://doi.org/10.1111/j.1095-8649.1972.tb05704.x
  2. De Boeck G, A. Vlaeminck and R Blust, 1997. Effects of sublethal copper exposure in copper accumulation, food consumption, growth, energy stores, and nucleic acid content in common carp. Arch. Environ. Contam. TaxicoI., 33, 415--422 https://doi.org/10.1007/s002449900271
  3. de la Torre, F. R., A. Salibian and L. Ferrari., 1999. Enzyme activities as biomarkers of freshwater pollution: responses of fish branchial (Na+K)-ATPase and liver transminases. Environ.Toxicol., 14, 313-319 https://doi.org/10.1002/(SICI)1522-7278(199907)14:3<313::AID-TOX4>3.0.CO;2-J
  4. de la Torre, F. R, A. Salibian and L. Ferrari, 2000. Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environ. Pollut., 109,277-282 https://doi.org/10.1016/S0269-7491(99)00263-8
  5. Duthie, G G and L. Tort, 1985. Effects of dorsal aortic cannulation on the respiration and haematology of Mediterranean living Scyliorhinus canicula. Compo Biochem. Physiol. A, 81, 879-885 https://doi.org/10.1016/0300-9629(85)90923-5
  6. Gill, T. S., J. Pande and H. Tewari, 1990. Enzyme modulation by sublethal concentrations of Aldicarb, Phosphamidon, and Endosulfan in fish tissues. Pestic. Biochem. PhysioI., 38, 231-244 https://doi.org/10.1016/0048-3575(90)90095-J
  7. Gill, T. S., H. Tewari and J. Pande, 1991. In vivo and in vitro effects of cadmium on selected enzymes in different organs of the fish Barbus conchonius Ham (rosy barb). Compo Biochem. Physiol. 100C, 501-505
  8. Jee, J.-H., S.-G Kim and J.-C. Kang, 2004. Effects of phrnanthrene on growth and basic physiological functions of the oliver flounder, Paralichthus olivaceus. J. Exp. Mar. BioI. Ecol., 304, 123-136 https://doi.org/10.1016/j.jembe.2003.12.001
  9. Kubin, L. A, 1989. Growth of juvenile English sole exposed to sediments amended with aromatic compounds. MS Thesis, Western Washington State University, 99 pp
  10. Lorenzen, K., 1996. The relationship between body weight and natural mortality in juvenile and adult fish: a comparison of natural ecosystems and aquaculture. J. Fish BioI., 49, 627--647 https://doi.org/10.1111/j.1095-8649.1996.tb00060.x
  11. McGurk, M. D., 1996. Allometry of marine mortality of Pacific salmon. Fish Bull., 94, 77-88
  12. Moon, T. W. and G. P. Foster, 1995. Tissue carbohydrate metabolism, gluconeogenesis and hormonal and environmental influences. In: Hochachka, P. w., Mommsen, T. P. (Eds.), Metabolic Biochemistry. Elsevier, Amsterdam, pp 65-100
  13. Morgan,J. D. and G K. Iwama, 1997. Measurements of stressed status in the field. In: Iwama, G K., Pickering, A D., Sumpter, J. P., Schreck, C. B. (Eds.), Fish Stress and Health in Aquaculture, Society for Experimental Biology Seminar Series, vol. 62
  14. Rice, C. A, M. S. Myers, M. L. Willis, B. L. French and E. Casilla, 2000. From sediment bioassay to fish biomarker-connecting the dots using simple trophic relationships. Mar. Environ. Res., 50, 527-533 https://doi.org/10.1016/S0141-1136(00)00122-7
  15. Roche, H. and G Boge, 2000. In vitro effects of phenolic compounds on blood parameters of a marine fish (Dicentrarchus labrax). Com. Biochem. Physiol., 125C, 345-353
  16. Svobodova, Z. and B. Vysukova, 1991. Diagnostics; prevention and therapy of fish disease and intoxications. Manual for International Training Course on Fish-Water Disease and Intoxications. Vodnany, Czechoslovaquia, 270 pp
  17. Wepender, V., J. H. J. Van Vuren and H. H. Preez, 1992. Effect of manganese and iron at neutral and acidic pH on the hematology of the banded tilapia (Tilapia sparrmanii). Bull. Environ. Contam. Toxicol., 49, 613--619 https://doi.org/10.1007/BF00196307
  18. Yoshida, Y, N. Itoh, M. Hayakawa, R. Piga, O. Cynshi, K. Jishage and E. Niki, 2005. Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE. Toxicol. Appl. Pharmacol., in press