Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Determination of Total Phenols in Environmental Waters by Capillary-HPLC with U.S.E.P.A. Classified Eleven Priority Pollutant Phenols after Nitrosation and Their Visible Spectrophotometric Detection

  • Published : 2005.02.20
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Abstract

The determination of total phenols was accomplished by capillary-high performance liquid chromatography (capillary-HPLC) after nitrosation of the U.S.E.P.A. classified 11 priority pollutant phenols, using the nitrosated parent phenol (POHNO) as a reference for calibration. The optimum mobile phase composition for this analysis was found by examining the effect of changing the percentage of acetonitrile (MeCN) in the mobile phase on retention factors (k values) and peak intensities. As MeCN percentage was increased, k values were reduced and peak intensities were generally increased. From the results obtained, it was found that the optimum mobile phase was 90%(v/v) MeCN solution at pH 8.0, the detection wavelength of 400 nm, and a detection limit (D.L., concentration at signal to noise ratio (S/N) of 3.0) of 4.5 ${\times}$ $10^{-7}$ M. In addition, 10 of the 11 phenols present in mineral or waste water were separated after the nitrosation by capillary-HPLC. The optimum mobile phase for separation was a 40%(v/v) MeCN solution at pH 5.0.

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References

  1. Sampling and Analysis Procedures for Screening of Industrial Effluents for Priority Pollutants, U.S.E.P.A.; Environmental Monitoring and Support Laboratory: Cincinnatti, OH, 1977
  2. Cremlyn, R. Pesticides: Preparation and Mode of Action; Wiley: Chichester, 1978
  3. Lindstrom, K.; Nordin, J. J. Chromatogr. 1976, 128, 13 https://doi.org/10.1016/S0021-9673(00)84026-1
  4. Abrahamsson, K.; Xie, T. M. J. Chromatogr. 1983, 279, 199 https://doi.org/10.1016/S0021-9673(01)93618-0
  5. Drinking Water Directive 80/778/EEC; Commission of the European Communities: Brussels, 1980
  6. American Public Health Association: Standard Methods for the Examination of Water and Waste Water, 16th ed.; Washington, D.C., 1985; part 510
  7. Koppe, P.; Dietz, F.; Traud, J.; Robelt, C. Fresenius J. Anal. Chem. 1977, 285, 1 https://doi.org/10.1007/BF00446011
  8. Frenzel, W.; Oleksy-Frenzel, J. Anal. Chim. Acta 1992, 261, 253 https://doi.org/10.1016/0003-2670(92)80199-H
  9. Zhao, C.; Song, J.; Zhang, J. Anal. Bioanal. Chem. 2002, 374, 498 https://doi.org/10.1007/s00216-002-1451-3
  10. Masque, N.; Pocurull, E.; Marce, R. M.; Borrull, F. Chromatographia 1998, 47(3/4), 176 https://doi.org/10.1007/BF02466578
  11. Ruana, J.; Urbe, I.; Borrull, F. J. Chromatogr. A 1993, 655, 217 https://doi.org/10.1016/0021-9673(93)83226-I
  12. Chung, Y.; Lee, S.; Motomizu, S. Anal. Sci. & Tech. 2004, 17(6), 393
  13. Chung, Y.; Lee, K. Microchem. J. 2001, 69, 143
  14. Chung, Y.; Chung, W. Bull. Korean Chem. Soc. 2003, 24(12), 1781 https://doi.org/10.5012/bkcs.2003.24.12.1781
  15. Chung, Y.; Kim, B.; Hwang, J.; Kim, C. S. Microchem. J. 1999, 62, 336 https://doi.org/10.1006/mchj.1999.1739
  16. Martinez, D.; Pocurull, E.; Marce, R. M.; Borrull, F.; Calull, M. Chromatographia 1996, 43(11-12), 619 https://doi.org/10.1007/BF02292977
  17. Pulg, D.; Silgoner, I.; Grasserbauer, M.; Barcel, D. Anal. Chem. 1997, 69, 2756 https://doi.org/10.1021/ac961256j
  18. Bigley, F. P.; Grob, R. L. J. Chromatogr. 1985, 350(2), 407 https://doi.org/10.1016/S0021-9673(01)93546-0

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