Bulletin of the Korean Chemical Society

  • 제26권3호
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  • Pages.379-383
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  • 2005
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip

  • Choi, Yong-Sung (School of Electrical, Electronics and Information of Engineering, WonKwang University) ;
  • Lee, Kyung-Sup (Dept. of Electrical Eng., Dongshin University) ;
  • Park, Dae-Hee (School of Electrical, Electronics and Information of Engineering, WonKwang University)
  • 발행 : 2005.03.20
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초록

This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from $10^{-18}\;M\;to\;10^{-5}$ M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.

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참고문헌

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  2. Target label-free, reagentless electrochemical DNA biosensor based on sub-optimum displacement vol.75, pp.2, 2008, https://doi.org/10.1016/j.talanta.2007.11.035
  3. An investigation on FR4 as a based material for Ti/Au and Cu/Au evaporated fabrication for DNA biosensor application vol.1535, pp.None, 2020, https://doi.org/10.1088/1742-6596/1535/1/012037