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Microcystin Detection Characteristics of Fluorescence Immunochromatography and High Performance Liquid Chromatography

  • Published : 2005.02.20

Abstract

Different detection characteristics of fluorescence immunochromatography method and high performance liquid chromatography (HPLC) method for the analysis of cyanobacterial toxins were studied. In particular, low and high limits of detection, detection time and reproducibility and detectable microcystin species were compared when fluorescence immunochromatography method and high performance liquid chromatography method were applied for the detection of microcystin (MC), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa. A Fluorescence immunochromatography assay system has the unique advantages of short detection time and low detection limit, and high performance liquid chromatography detection method has the strong advantage of individual quantifications of several species of microcystins.

Keywords

References

  1. Carmichael, W. W. In Marine Toxins and Venoms, Handbook of Natural Toxins; Tu, A. T., Ed.; Dekker: New York, 1988; Vol 3, pp 121-147
  2. Falconer, I. R.; Jackson, A. R. B.; Langley, J.; Runnegar, M. T. C. Aust. J. Biol. Sci. 1981, 34, 179
  3. Eriksson, J. E.; Toivola, D.; Meriluoto, J. A. O.; Haraki, H.; Han, Y.; Hartshorne, D. Biochem. Biophys. Res. Commun. 1990, 173, 1347 https://doi.org/10.1016/S0006-291X(05)80936-2
  4. Carmichael, W. W. United States Environmental Protection Agency/600/R-92/079 1992, 32-33
  5. Matsushima, R.; Yoshizawa, S.; Watanabe, M. F.; Harada, K.; Furusawa, M.; Carmichael, W. W.; Fujiki, H. Biochem. Biophys. Res. Commun. 1990, 171, 867 https://doi.org/10.1016/0006-291X(90)91226-I
  6. Hooser, S. B.; Kuhlenschmidt, M. S.; Dahlem, A. M.; Beasly, V. R.; Carmichael, W. W.; Haschek, W. M. Toxicon 1991, 29, 589 https://doi.org/10.1016/0041-0101(91)90053-T
  7. Yoshizawa, S.; Matsushima, R.; Watanabe, M. F.; Harada, K.; Ichihara, A.; Carmichael, W. W.; Fujiki, H. J. Cancer Res. Clin. Oncol. 1990, 116, 609 https://doi.org/10.1007/BF01637082
  8. Honkanen, R. E.; Zwiller, J.; Moore, R. E.; Daily, S. L.; Khatra, B. S.; Dukelow, M.; Boynton, A. L. J. Biol. Chem. 1990, 265, 19401
  9. MacKintosh, C.; Beattle, K. A.; Klumpp, S.; Cohen, P.; Codd, G. A. FEBS Lett. 1990, 264, 187 https://doi.org/10.1016/0014-5793(90)80245-E
  10. Nishiwaki-Matsushima, R.; Ohta, S.; Nishiwaki, S.; Suganuma, M.; Kohyama, K.; Ishiwaka, T.; Carmichael, W. W.; Fujiki, H. J. Cancer Res. Clin. Oncol. 1992, 118, 420
  11. Falconer, I. R. Environ. Toxic Water Qual. 1991, 6, 177 https://doi.org/10.1002/tox.2530060207
  12. Carmichael, W. W. Sci. Am. 1994, 1, 64
  13. Yu, S. Z. In Primary Liver Cancer; Tang, Z. Y.; Wu, M. C.; Xia, S. S.; Eds.; China Academic Publishers/Springer: Berlin, 1989; pp 1- 37
  14. Ueno, Y.; Nagata, S.; Tsutsumi, T.; Hasegawa, A.; Watanabe, M. F.; Park, H. D.; Chen, G. C.; Chen, G.; Yu, S. Z. Carcinogenesis 1996, 17, 1317 https://doi.org/10.1093/carcin/17.6.1317
  15. Watanabe, M. F.; Oishi, S.; Harada, K.; Matsuura, K.; Kawai, H.; Suzuki, M. Toxicon 1988, 26, 1017 https://doi.org/10.1016/0041-0101(88)90200-0
  16. Meriluoto, J. A.; Eriksson, J. E.; Harada, K.; Dahlem, A. M.; Sivonen, K.; Carmichael, W. W. J. Chromatogr. 1990, 509, 390 https://doi.org/10.1016/S0021-9673(01)93097-3
  17. Kondo, F.; Ikai, Y.; Oka, H.;Watanabe, M. F.; Watanabe, M.; Harada, K.; Suzuki, M. Toxicon 1992, 30, 227 https://doi.org/10.1016/0041-0101(92)90865-3
  18. Pyo, D.; Choi, E.; Cha, G. S.; Lee, J.; Jung, S.; Kim, M. S. Bull. Korean Chem. Soc. 2003, 24, 126 https://doi.org/10.5012/bkcs.2003.24.1.126
  19. McDermott, C. M.; Feola, R.; Plude, J. Toxicon 1995, 33, 1433 https://doi.org/10.1016/0041-0101(95)00095-4

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