한국잠사곤충학회지 (Journal of Sericultural and Entomological Science)

한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

백강잠(白彊蠶) 생산을 위한 Beauveria bassiana의 효율적인 접종법 및 백장잠의 간보호 활성 검정

Efficient Inoculation Method of Beauveria bassiana for Production of Bombycis corpus and Evaluation of Its Liver Protection Activity

  • 정이연 (농업과학기술원 농업생물부) ;
  • 홍인표 (농업과학기술원 농업생물부) ;
  • 강필돈 (농업과학기술원 농업생물부) ;
  • 남성희 (농업과학기술원 농업생물부) ;
  • 김미자 (농업과학기술원 농업생물부)
  • Jung, I-Yeon (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Hong, In-Pyo (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kang, Pil-Don (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Nam, Sung-Hee (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Mi-Ja (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
  • 발행 : 2005.06.01
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초록

이 연구는 백강잠(B. corus)의 대량생산을 위한 B. bassiana의 효과적인 접종법을 구명하고 백강잠의 간보호 활성을 조사하기 위하여 실시하였던 바 다음과 같은 결과를 얻었다. 1. 백강잠 대량생산을 위한 B. bassiana의 접종시험에서 포자농도를 1.0${\times}$$10^8$ spores/m/로 할 경우 접종후 고습도 처리시간에 관계없이 높은 감염율을 나타내었으나, 1.0${\times}10^7$ spores/m/의 농도에서는 감염율이 낮았다. 2. B. bassiana 종균의 보관온도 및 보관일수별 활력 검정에서 $4^{\circ}C$에서는 12일까지 보관시 감염률 90% 이상 유지하였으나, 상온($25^{\circ}C$ 내외) 보관시에는 48시간이 지나면 감염율이 급격히 저하(5% 이하)하였다. 또한 모균주 보존기간별 감염율은 $4^{\circ}C$에 12개월 보존시에도 초기값과 활력에 차이가 없었으며 감염율 역시 90% 이상으로 균보관은 $4^{\circ}C$에서 12개월까지 보관하여도 활력에는 별 차이가 없었다. 3. B. bassiana 101A의 간기능 보호활성도 측정에서 galactosamine으로 독성을 유발한 처리구에서는 물분획층에서 대조물질 silymarin과 DDB 대비 각각 43.5%, 65.7%의 간보호 회복 효과를 나타내었으며 사염화탄소($CCl_4$)로 독성을 유발한 처리구에서는 에틸아세테이트 분획층에서 대조 물질대비 각각 100%, 69.3%의 간보호 회복 효과가 있었다.

When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.

키워드

참고문헌

  1. Barnett, H. L. and B. B. Hunter (1986) Illustrated genera of imperfect fungi. Publishing company, New York. and Collier macmillan publishers, London 100-101
  2. Doberski, J. W. and H. T. Tribe (1980) Isolation of entomogenous fungi from elm bark and soil with reference to ecology of Beauveria bassiana and Metarhizium anisopliae. Trans. Br. Mycol. Soc.,74(1): 95-100 https://doi.org/10.1016/S0007-1536(80)80013-1
  3. Edsall, J. T. (1943) Raman spectra of amino acids and related compounds. VI. Sarcosine, ethanolamine, choline, betaine and betaine derivatives. J. Am. Chem. Soc., 65: 1767 https://doi.org/10.1021/ja01249a029
  4. Ferron, P. (1967) Etude en la boratoire des conditions ecologigues favorisant Ie development de lamycose a Beauveria. tenella du ver Blanc. Entomophaga 12(3): 257-293 https://doi.org/10.1007/BF02371432
  5. Hefti, F. (1994) Neurotrophic factor therapy for nerve system degenerative diseases, J. Neurobiol., 25: 1418 https://doi.org/10.1002/neu.480251109
  6. Isobe, R.,M. Inagaki, Y. Harano, H. Sakiyama and R. Higuchi (1997) Structural elucidation of glycosphingolipids by collision-induced dissociation of sodium ion complex. Chemical & Pharmaceutical Bulletin 45: 1611-1614 https://doi.org/10.1248/cpb.45.1611
  7. Jiang, H., X. Huang, K. Nakanishi and N. Berova (1999) Nanogram scale absolute configurational assignment of cerami des by circular dichroism. Tetrahedron Letters 40: 7645-7649 https://doi.org/10.1016/S0040-4039(99)01581-6
  8. Kiso, Y.,M. Tohkin and H. Hikino (1983) Assay method for anti-hepatotoxic activity using carbon tetrachloride induced cytotoxicity in cultured hepatocytes. Planta Med. 49(4): 189-222
  9. Krivit, W. and S. Hammarstrom (1972) Identification and quantitation of free ceramides in human platelets. Journal of Lipid Research 13: 525-530
  10. Kwon, H. C., H. I. Moon, S. H, Choi, J. O. Lee, S. Y. Cho, I. Y. Jung, S. Y. Kim and K. R. Lee (2000) Cytotoxic constituents of Bombycis corpus. Yakhak Hoeji, 43: 169
  11. Kwon, H. C., E. J. Bang, S. U. Choi, W. C. Lee, S. Y, Cho, I. Y. Jung, S. Y. Kim and K. R. Lee (2000) Cytotoxic cyclodepsipeptides of Bombycis corpus 101A. Yakhak Hoeji, 44: 115
  12. Lecuona, R., G. Riba, P. Cassier and J. L. Clement (1991) Alterations of insect epicuticular hydrocarbons during infection with Beauveria bassiana or B. brongniartii. J. Invertebr. Pathol. 58: 10-18 https://doi.org/10.1016/0022-2011(91)90156-K
  13. Leifer, A. and E. R. Lippincott (1957) The infrared spectra of some amino acids. J. Am. Chem. Soc., 79: 5098 https://doi.org/10.1021/ja01576a006
  14. Lindavall, O., Z. Kokaia, J. Bengzon, E. Elmer and M. Kokaia (1994) Neurotrophins and brain insults. Trends Neurosci., 17: 490 https://doi.org/10.1016/0166-2236(94)90139-2
  15. Motohashi, N., I. Mori and Y. Sugiura (1976) $^{13}C-Nuclear magnetic resonance and raman spectroscopic studies on ionization and mercury complex of ergothioneine. Chem. Pharm. Bull., 24: 1737 https://doi.org/10.1248/cpb.24.1737
  16. Roberts, D. W.(1989) World picture of biological control of fungi. Inst. Owaldo Cruz, Rio de Janeiro 84(Supl. III): 89-100 https://doi.org/10.1590/S0074-02761989000700018
  17. Samsinakova, A. Y. and E. Hrabetova (1969) Respiration of blastospores of the fungus Beauveria bassiana during submersed cultivation in the presence of certain sugar. J. Invertebr. Pathol. 13: 382-385 https://doi.org/10.1016/0022-2011(69)90190-6
  18. Samsinakova, A. (1966) Growth and sporulation of submersed cultures of the fungus Beauveria bassiana in various media. J. Invertebr. Pathol. 8: 395-400 https://doi.org/10.1016/0022-2011(66)90056-5
  19. Samson, R. A., H. C. Erans and J. P. Latg (1988) Atlas of Entomopathogenic Fungi. Springer-Verlags, Berlin
  20. Shimazu, M., W. Mitsuhashi and H. Hashimoto (1988) Cordyceps brongniartii. Trans. Mycol. Soc. Japan. 29: 323-330
  21. Steinhaus, E. A. (1949) Principles of Insect Pathology. McGraw-Hill Book, New York
  22. Studdert, J. P. and H. K. Kaya (1990) Water potential, temperature, and soil type on the formation of Beauveria bassiana soil colonies. J. Invertebr. Pathol. 56: 380-386 https://doi.org/10.1016/0022-2011(90)90125-P
  23. Tanada, Y. and H. Kaya (1992) Insect pathology, Academic press. 357-359
  24. Vuillemin, P. (1912) Beauveria, nouveau genre de Verticilliacees. Paris Soc. Bot. Fr. Bull. 59: 34-40 https://doi.org/10.1080/00378941.1912.10832379
  25. Bidochka, M. J. and G. G. Khachatourians (1987) Hemocytic defens respons to the entomopathogenic fungus Beauveria bassiana in the migratory grasshopper Melanoplus sanguinipes. Entomol. Exp. Appl. 45: 151-156 https://doi.org/10.1007/BF00305641
  26. Ferron, P. (1985) Fungal control. In Comprehensive Insect Physiology Biochemistry and Pharmacology. (G. A. Kerkut and L. I. Gilbert) Vol. 12,pp. 313-346. Pergamon Press, Oxford
  27. 島根孝典, 河上 淸 (1993) 곤충병원사상균 Beauveria brongniartii の蠶およびマウス に對す る安全性 について. J. Seri. Sci. Jpn. 62(1): 30-37
  28. 淸水大典(1994) 原色冬蟲夏草圖鑑. 誠文堂新光社, pp.1-381
  29. 김창민, 신민교, 안닥균, 이경순 (1997) 중약대사전, 완역판, 도서출판 정담, pp. 2015
  30. 배경숙 (1997) 미생물 동정 결과 보고서. Korean Collection For Type Cultures
  31. 조세연, 신국현, 송성규, 성재모(1999) 누에동충하초 생산 및 유용물질 개발. 농촌진흥청, pp.42-64