International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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A LIM Protein Gene Homologue of Protaetia brevitarsis: cDNA Cloning and mRNA Expression

  • Kim Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Choi Yong Soo (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee Sun Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Kim Mi Ae (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Kim Seong Ryul (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Hwang Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2005.09.01
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Abstract

A LIM protein gene homologue of the CRP (cysteine­rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypep­tide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRPl). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92$\%$ identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.

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References

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