International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Amino Acid Alterations in the $\beta$- Tubulin of Metarhizium anisopliae That Confer Benomyl Resistance

  • Kim Soon Kee (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Shim Hee Jin (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Roh Jong Yul (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Li Ming Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi Jae Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Boo Kyung Saeng (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Je Yeon Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
  • Published : 2005.09.01
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Abstract

We cloned the $\beta$-tubulin genes from the wild type strain and two benomyl-resistant mutants of Metahizium anisopliae and determined their nucleotide sequences. A $\beta$-tubulin encoding 448-residue protein from wild type M. anisopliae shows strong homology to other $\beta$-tubulins. The coding region is interrupted by four introns. Comparisons of intron position between the M. anisopliae gene and other fungal $\beta$-tubulin genes show considerable positional conservation. The mutations responsible for benomyl resistance were determined in two spontaneous mutants, 8-18 and 8­19. One mutant 8-18 substituted glutamate for aspar­agine at position 33 and lysine for glutamine at position 134. The other mutant 8-19 showed alterations at three positions of $\beta$-tubulin arginine for tryptophan at position 21, lysine for asparagine at position 33, and phenylalanine for leucine at position 240. These data suggest that regions of $\beta$-tubulin containing amino acids 21, 33,134, and 240 interact to form the binding site of benomyl.

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