Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Hepatoprotective Properties of Allium tuberosum in CCl$_{4}$ intoxicated Rats

$CCl_{4}$ 투여 흰쥐에서 부추의 간보호 작용

  • Ha Hun (Department of Pharmacy, Sangrok Hospital) ;
  • Yoon Soo Hong (Department of Pharmacy, Catholic University of Taegu)
  • 하헌 (상록병원 약제과) ;
  • 윤수홍 (대구가톨릭대 약학과)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

대표적 간장해 유발 물질인 사염화탄소로 유도된 급성 간독성에 미치는 한국산 부추(Alluim tuberosum) 에탄을 추출물의 효과를 관찰하였다. 사염화탄소 투여로 혈청 AST (aspartate transaminale), ALT (alanine tranlaminase)와 ALP(alkaline phosphatase)활성이 현저히 증가하였으며,부추 에탄을 추출물의 투여(50, 100, 200mg/kg)로 특히 CCl$_{4}$에 의해 유도된 AST와 ALT의 활성이 농도 의존적으로 회복되었다. 사염화탄소 투여로 감소된 SOD (superoxide dismutase)와 catalase 활성은 부추의 에탄을 추출물 투여로 유의성 있는 활성 증가를 나타내어 간장해로 인한 산화적 스트레스가 부추의 항산화작용에 의해 완화되었음을 시사하였다. 결과적으로 부추 ethanol 추출물의 간보호 및 항산화작용으로 사염화탄소로부터의 간장해를 개선시키는 것으로 사료된다.

Keywords

References

  1. Bandyopadhyay U, Das D and Banerjee RK. Reactive oxygen species: oxidative damage and pathogenesis, Curr Sci 1999; 77: 658-665
  2. Chance B, Greenstein DS, and Roughton RJW. The mechanism of catalase action-steady state analysis, Arch Biochem Biophys 1952; 37: 301- 339 https://doi.org/10.1016/0003-9861(52)90194-X
  3. Cohen G, Demblic D and Marcus J. Measurement of catalase activity in tissue extracts, Anal Biochem 1970; 34: 30-38 https://doi.org/10.1016/0003-2697(70)90083-7
  4. Fenwick GR and Hanley AB. The genus Allium-Part I, Cri Rev Food Sci Nutr 1985; 22: 199-271 https://doi.org/10.1080/10408398509527415
  5. Kind PRM and King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino antipyrine, J Clin Pathol 1954; 7: 322-326 https://doi.org/10.1136/jcp.7.4.322
  6. Lowry OH, Rosebrough HJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent, 1951; 193: 265-275
  7. Marklund S and Marlund G. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for SOD, Eur J Biochem 1974; 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  8. Mehendale HM, Roth RA, Gandolfi AJ, Kraunig JE, Lemasters JJ and Curtis LR. Novel mechanism in chemically induced hepatotoxicity, FASEB J 1986; 104: 826-829
  9. Movahedian A, Ghannadi A, Sadeghi H, Gharavi M and Azarpajhoh S. Study of Leek extract on cholesterol plasma levels in hyperlipidemic animals, Asia Pac J Clin Nut 2004; 13(suppl): S103
  10. Ohta Y, Sasaki E, Nishida K, Kobayashi T, Nagata M and Ishigur I. Preventive effect of dia-saiko-to (da-chaihu-tang) extract on disrupted hepatic active oxygen metabolism in rats with carbon tetrachloride induced liver injury, Am J Chin Med 1995; 23: 53-64 https://doi.org/10.1142/S0192415X95000080
  11. Paduraru I, Saramet A, Danila GH, Nichifor M, Jerca L, Iacobovici A, Ungureanu D and Filip M. Antioxidant action of a new flavonic derivative in acute carbon tetrachloride intoxication, Eur J Drug Metab Pharmacokinet 1996; 21: 1-6 https://doi.org/10.1007/BF03190270
  12. Recknagel RO, Glende EA Jr, Dolak JA and Walker RL. Mechanisms of carbon tetrachloride toxicity, Pharmacol Ther 1989; 43: 139-154 https://doi.org/10.1016/0163-7258(89)90050-8
  13. Reitman S and Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetate and serum glutamate pyruvate transaminase, Am J Clin Pathol 1957; 28: 56-75
  14. Shanghai Science & Technical Publisher, The Dictionary of Chinese Drugs Shougakukan, Tokyo, 1985, 449
  15. Sherlock S. Biochemical assessment of liver function, Blackwell Scientific. Publication, Oxford, 1981, 14
  16. Szymonik-Lesiuk S, Czechowska G, Stryjecka-Zimmer M, Slomka M, Madro A, Celinski K and Wielosz. Catalse, superoxide dismutase and glutathione peroxidase activities in varius rat tissues after carbon tetrachloride in toxication, J Hepatobilliary Pancreat Surg 2003; 10: 309-315 https://doi.org/10.1007/s00534-002-0824-5
  17. Van Remmen H, Williams MD, Guo Z, Esttack L, Yang H, Carlson EJ, Epstein CJ, Huang TT and Richardson A. Knockout mice heterozygous for Sod2 show alterations in cardiac mitochondrial function and apoptosis, Am J physiol Heart Circ Physiol 2004; 281: H1422-1432
  18. Wolf PL. Biochemical diagnosis of liver disease, Indian J Clin Biochem 1999; 14: 59-65 https://doi.org/10.1007/BF02869152