Journal of Microbiology

  • 제43권3호
  • /
  • Pages.219-227
  • /
  • 2005
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Isolation and Characterization of Bacteria Associated with Two Sand Dune Plant Species, Calystegia soldanella and Elymus mollis

  • Park Myung Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung Se Ra (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Myoung Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Kyoung Ok (Korea Research Institute of Bioscience and Biotechnology) ;
  • Do Jin Ok (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Kang Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Seung Bum (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Bae Kyung Sook (Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2005.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Little is known about the bacterial communities associated with the plants inhabiting sand dune ecosystems. In this study, the bacterial populations associated with two major sand dune plant species, Calystegia soldanella (beach morning glory) and Elymus mollis (wild rye), growing along the costal areas in Tae-An, Chungnam Province, were analyzed using a culture-dependent approach. A total of 212 bacteria were isolated from the root and rhizosphere samples of the two plants, and subjected to further analysis. Based on the analysis of the 16S rDNA sequences, all the bacterial isolates were classified into six major phyla of the domain Bacteria. Significant differences were observed between the two plant species, and also between the rhizospheric and root endophytic communities. The isolates from the rhizosphere of the two plant species were assigned to 27 different established genera, and the root endophytic bacteria were assigned to 21. Members of the phylum Gammaproteobacteria, notably the Pseudomonas species, comprised the majority of both the rhizospheric and endophytic bacteria, followed by members of Bacteroidetes and Firmicutes in the rhizosphere and Alphaproteobacteria and Bacteroidetes in the root. A number of isolates were recognized as potentially novel bacterial taxa. Fifteen out of 27 bacterial genera were commonly found in the rhizosphere of both plants, which was comparable to 3 out of 21 common genera in the root, implying the host specificity for endophytic populations. This study of the diversity of culturable rhizospheric and endophytic bacteria has provided the basis for further investigation aimed at the selection of microbes for the facilitation of plant growth.

키워드

참고문헌

  1. Bakker, A.W. and P. Schippers. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Psudomonas spp.-mediated plant growth-stimulation. Soil Biol. Biochem. 19, 451-457 https://doi.org/10.1016/0038-0717(87)90037-X
  2. Chanway, C.P., L.M. Nelson, and F.B. Holl. 1988. Cultivar-specific growth promotion of spring wheat (Triticum aestivum L.) by coexistent Bacillus species. Can. J. Microbiol. 34, 925-929 https://doi.org/10.1139/m88-164
  3. Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph. D. thesis, University of Newcastle, Newcastle upon Tyne, UK
  4. Cieslinski, G., K.C.J. Van-Rees, A.M. Szmigielska, and P.M. Huang. 1997. Low molecular weight organic acids released from roots of durum wheat and flax into sterile nutrient solutions. J. Plant Nutr. 20, 753-764 https://doi.org/10.1080/01904169709365291
  5. Dalton, D.A., S. Kramer, N. Azios, S. Fusaro, E. Cahill, and C. Kennedy. 2004. Endophytic nitrogen fixation in dune grasses (Ammophila arenaria and Elymus mollis) from Oregon. FEMS Microbiol. Ecol. 49, 469-479 https://doi.org/10.1016/j.femsec.2004.04.010
  6. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791 https://doi.org/10.2307/2408678
  7. Germida, J.J., S.D. Siciliano, J.R. de Freitas, and A.M. Seib. 1998. Diversity of root-associated bacteria associated with fieldgrown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microbiol. Ecol. 26, 43-50 https://doi.org/10.1111/j.1574-6941.1998.tb01560.x
  8. Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41, 109-117 https://doi.org/10.1139/m95-015
  9. Grayston, S.J., D. Vaughan, and D. Jones. 1996. Rhizosphere carbon flow in tree, in comparison with annual plants, the importance of root exudation and its impact on microbial diversity and nutrient availability. Appl. Soil Ecol. 5, 29-56 https://doi.org/10.1016/S0929-1393(96)00126-6
  10. Grayston, S.J., S. Wang, C.D. Campbell, and A.C. Edwards. 1998. Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol. Biochem. 30, 369-378 https://doi.org/10.1016/S0038-0717(97)00124-7
  11. Hallmann, J., A. Quadt-Hallmann, W.F. Mahaffee, and J.W. Kloepper 1997. Bacteria endophytes in agricultural crops. Can. J. Microbiol. 43, 895-914 https://doi.org/10.1139/m97-131
  12. Jacobson, C.B., J.J. Pasternak, and B.R. Glick. 1994. Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth promoting rhizobacterium Psudomonas putida GR12-2. Can. J. Microbiol. 40, 1019-1025 https://doi.org/10.1139/m94-162
  13. Jeon, J.-S., S.-S. Lee, H.-Y. Kim, T.-S. Ahn, and H.-G. Song. 2003. Plant growth promotion in soil by some inoculated microorganisms. J. Microbiol. 41, 271-276
  14. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16, 111-120 https://doi.org/10.1007/BF01731581
  15. Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt and M. Goodfellow (eds.), Nucleic Acid Techniques in Bacterial Systematics. John Weley & Sons, Chichester, UK
  16. Lee, D.-H., S.-A. Noh, and C.-K. Kim. 2000. Development of molecular biological methods to analyze bacterial species diversity in freshwater and soil ecosystems. J. Microbiol. 38, 11-17
  17. Lemanceau, P., T. Corbererand, L. Gardan, X. Latour, G. Laguerre, J-M. Boeufgras, and C. Alabouvette. 1995. Effect of two plant species, flax (Linum usitatissinum) and tomato (Lycopersicon esculentum Mill) on the diversity of soilborne populations of fluorescent Pseudomonas. Appl. Environ. Microbiol. 61, 1004-1012
  18. Lilley, A.K., J.C. Fry, M.J. Bailey, and M.J. Day. 1996. Comparison of aerobic heterotrophic taxa isolated from four root domains of mature sugar beet (Beta vulgaris). FEMS Microbiol. Ecol. 21, 231-242 https://doi.org/10.1111/j.1574-6941.1996.tb00350.x
  19. Lim, J.-S., M.-K. Jung, M.-S. Kim, J.-H. Ahn, and J.-O. Ka. 2004. Genetic and phenotypic diversity of (R/S)-mecoprop [2-(2-methyl-4-chlorophenoxy)propionic acid]-degrading bacterial isolated from soils. J. Microbiol. 42, 87-93.
  20. Lucy, M., E. Reed, and B.R. Glick. 2004. Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek. 86, 1-25 https://doi.org/10.1023/B:ANTO.0000024903.10757.6e
  21. Mahaffee, W.F. and J.W. Kloepper. 1997. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with field-grown cucumber (Cucumis sativus L.). Microb. Ecol. 34, 210-223 https://doi.org/10.1007/s002489900050
  22. Maloney, P.E., A.H.C. Van Bruggen, and S. Hu. 1997. Bacterial community structure in relation to the carbon environments in lettuce and tomato rhizosphere and in bulk soil. Microb. Ecol. 34, 109-117 https://doi.org/10.1007/s002489900040
  23. Mantelin, S. and B. Touraine. 2004. Plant growth-promoting bacteria and nitrate availability; impacts on root development and nitrate uptake. J. Exp. Bot. 55, 27-34 https://doi.org/10.1093/jxb/erh010
  24. Marilley, L. and M. Aragno. 1999. Phylogenetic diversity of bacterial communities differing in degree of proximity of Lolium perenne and Trifolium repens roots. Appl. Soil Ecol. 13, 127-136 https://doi.org/10.1016/S0929-1393(99)00028-1
  25. Marschner, P., C.H. Yang, R. Lieberei, and D.E. Crowley. 2001. Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol. Biochem. 33, 1437-1445 https://doi.org/10.1016/S0038-0717(01)00052-9
  26. Merbach, W., E. Mirus, G. Knof, R. Remus, S. Ruppel, R. Russow, A. Gransee, and J. Schulze. 1999. Release of carbon and nitrogen compounds by plant roots and their possible ecological importance. J. Plant Nutr. Soil Sci. 162, 373-383 https://doi.org/10.1002/(SICI)1522-2624(199908)162:4<373::AID-JPLN373>3.0.CO;2-#
  27. Miethling, R., G. Weiland, H. Backhaus, and C.C. Tebbe. 2000. Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L 33. Microb. Ecol. 40, 43-56 https://doi.org/10.1007/s002480000021
  28. Park, H.-D. and J.-O. Ka. 2003. Genetic and phenotypic diversity of dichloroprop-degrading bacteria isolated from soils. J. Microbiol. 41, 7-15
  29. Read, D.J. 1998. Mycorrhizas and nutrient cycling in sand dune ecosystems. Coastal Sand Dunes, vol. 96, p. 89-110. In C.H. Gimmingham, W. Ritchie, B.B. Willetts and A.J. Willis (eds.), Proceedings of Symposium. The Royal Society of Edinburgh, Edinburgh
  30. Siciliano, S.D. and J.J. Germida. 1999. Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapa cv. Parkland. FEMS Microbiol. Ecol. 29, 263-272 https://doi.org/10.1111/j.1574-6941.1999.tb00617.x
  31. Singh, U.P., B.K. Sarma, and D.P. Singh. 2003. Effect of plant-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cier arietinum). Curr. Microbiol. 46, 131-140 https://doi.org/10.1007/s00284-002-3834-2
  32. Smith, S.E. and D.J. Read. 1997. Mycorrhizal Symbiosis. Academic Press, San Diego, California, USA
  33. Sylvia, D.M. and N.J. Burks. 1988. Selection of a vesicular-arbuscular mycorrhizal fungus for practical inoculation of Uniola paniculata. Mycologia 80, 565-568 https://doi.org/10.2307/3807859
  34. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876-4882
  35. Torsvik, V., J. Gokoyr, and F.L. Daae. 1990. High diversity in DNA of soil bacteria. Appl. Environ. Microbiol. 56, 782-787
  36. Van Loon, L.C., P.A. Bakker, and C.M. Pieterse. 1998. Systemic resistance induced by rhizosphere bacteria. Ann. Rev. Phytopathol. 36, 453-483 https://doi.org/10.1146/annurev.phyto.36.1.453
  37. Wei, G.J., W. Kloepper, and S. Tuzun. 1996. Induced systemic resistance to cucumber disease and increased plant growth by plant growth-promoting rhizobacteria under field condition. Phytopathology 86, 221-224 https://doi.org/10.1094/Phyto-86-221
  38. Whipps, J.M. 1990. Carbon economy, p. 59-97. In J.M. Lynch (ed.), The Rhizosphere. John Wiley & Sons, Essex, UK
  39. Whipps, J.M. 2001. Microbial interaction and biocontrol in the rhizosphere. J. Exp. Bot. 52, 487-511 https://doi.org/10.1093/jexbot/52.suppl_1.487