미생물학회지 (Korean Journal of Microbiology)

  • 제41권2호
  • /
  • Pages.152-156
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  • 2005
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

신생아 분변에서 분리한 Pediococcus acidilactici GMB7330의 Helicobacter pylori에 대한 항균활성

Anti-Helicobacter pylori Activity of Pediococcus acidilactici GMB7330 Isolated from Infant Feces

  • 강지희 (부경대학교 미생물학과) ;
  • 이명숙 (부경대학교 미생물학과)
  • Kang Ji-Hee (Department of Microbiology, Pukyong National University) ;
  • Lee Myung-Suk (Department of Microbiology, Pukyong National University)
  • 발행 : 2005.06.01
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초록

신생아의 분변에서 분리한 Pediococcus acidilacti GMB7330은 내산성(pH 2-3) 및 내담즙성$(1.0\%)$을 가지며 Helicobacter pylori에 대해 항균 활성을 나타내었다. P. acidilactici GMB7330의 H. pylori에 대한 저해 특성을 H. Pylori의 균수와 urease 활성 변화, 그리고 전자현미경을 이용한 세포구조 파괴를 관찰하므로써 조사하였다. P. acidilactici GMB 7330의 배양여액(pH 4.5)은 배양 24시간만에 H. pylori의 중식을 약 4 log 감소시켰다. 이때 P. acidilactici GMB7330의 배양여액의 pH를 중성으로 조절하였을 경우에는 pH 4.5의 경우보다 항균력이 다소 감소하기는 하였으나 완전히 소실되지는 않았다. 뿐만 아니라, P. acidilactici GMB7330과 유사한 농도의 젖산을 생성하는 유산균주 Lactobacillus sp. GM7311의 경우 H. pylori에 대해서 항균력을 나타내지 않는 것으로 미루어 P. acidilactici GMB7330의 항균 활성에는 배양 중 생성된 젖산 이외의 물질이 관여하는 것으로 관찰되었다. 또한 P. acidilactici GMB7330은 pH값에 관계없이 H. pylori의 urease 활성을 $50\%$이상 감소시켰으며, P. acidilactici GMB7330의 배양여액을 처리한 H. pylori 균주는 세포벽 구조의 파괴로 인한 세포 내부물질의 유출이 관찰되었다.

In the present study, lactic acid bacterium that has antibacterial activity against Helicobacter pylori was isolated from feces of newborn baby. The selection was based on the ability to inhibit the growth of H. pylori and to withstand harsh environmental conditions such as acidic pH and high bile concentration. By biochemical test and 16S rDNA sequencing, selected strain was turned out to be an Pediococcus acidilactici, therefore designated to P. acidilactici GMB7330. In order to investigate the inhibitory effects of P. acidilactici GMB7330 on the growth of H. pylori, we have tested in vitro studies such as cell viability and urease test. These results showed that antibacterial activity of P. acidilactici GMB7330 significantly decreased the viable cell count and urease activity of H. pylori. Antibacterial activity of P. acidilactici GMB7330 against H. pylori remained after pH adjustment to neutral, and the concentration of lactate produced from P. acidilactici GMB7330 was not enough to inhibit H. pylori. On the basis of the analysis by transmission electron microscope, it demonstrated that addition of P. acidilactici GMB7330 destroyed the cell structure of H. pylori. These results strongly suggested that P. acidilactici GMB7330 produce antibacterial substances to be able to inhibit the growth of H. pylori other than lactic acid.

키워드

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