Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Salinity-stratified Waters on Upward Migration and Ratio of Extracted DNA/RNA in Cochlodinium polykrikoides Margalef Based on the Ratio of Absorbance at 260 and 280nm

염분 구배가 Cochlodinium polykrikoides Margalef의 수직이동 및 DNA/RNA 비율에 미치는 영향

  • 조은섭 (국립수산과학원 남해수산연구소) ;
  • 이영식 (국립수산과학원 남해수산연구소)
  • Published : 2005.06.01
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Abstract

The coastal regions of Yeosu, the South Sea of Korea, has occurred annually the red tide which is caused by potentially ichthyotoxic dinoflagellate C. polykrikoides, with a wide avenue for exchange with oceanic waters and freshwater runoff from Sumjin river. We attempted to examine the variability in response to vertical migration and concentration of extracted DNA/RNA of C. polykrikoides exposed to salinity-stratified waters. The experimental aquarium of the 60 liter was employed to culture C. polykrikoides. One aquarium was supplied with only sea water, the other was consisted of sea water and freshwater. Experiment was conducted for 5 days. In experimental column (mixture of freshwater and sea water), salinity was maintained to 20 at upper and approximately 30 at bottom during the period of this study. The fluctuation with related to dissolved oxygen and pH was similar pattern to both columns. Chlorophyll a was significantly higher value at upper than bottom. During 24h, chlorphyll a on experimental column was extremely high on the top as soon as lighting, compared with control. With elapsed time, the gap between experimental and control columns was a little. In darkness, chlorophyll a was not significantly different between upper and bottom, most cells appeared to randomly distribute on column regardless of water layer. Fluctuation with related to concentration of extracted DNA and RNA based on ratio of absorbance of 260 and 280 nm in experimental column was higher at final day or diel migration than control. These results implied that a large volume of freshwater could be associated with influence of concentration of DNA and RNA, in particular, rapid upward movement caused massive fish kills as soon as sunset.

여수연안은 어류 치사성 유해적조생물인 C. polykrikoides 적조가 매년 발생되는 해역일 뿐만 아니라 섬진강으로부터 담수유입이 돌산을 중심으로 많은 영향을 받고 있다. 담수유입에 따른 염분도 변화가 C. polykrikoides의 수직이동에 영향을 미칠 수 있는 지를 조사했다. 실험장치는 60l들이 아크릴 수조에 일반해수와 담수와 해수를 혼합시킨 시험구를 설정하여 C.polykrikoides의 수직부상 속도와 DNA/RNA함량 비교를 하였다. 실험은 5일 동안 시행했다. 실험개시로부터 종료시까지 시험구의 경우 상층부위에서는 염분이 20으로 나타난 반면에, 하층은 30 정도로 유지되었다. 그러나 용존산소와 pH 변화는 시험구와 대조구 모두 비슷한 양상을 보였다. 또한 chlorophyll a도 수조의 하층보다 상층에 높은 수치를 보였다. 24시간동안 C. polykrikoides 상$\cdot$하층 이동을 조사한 결과 점등이 되는 순간 시험구는 대조구에 비하여 월등히 높은 상승을 보였으나, 시간이 경과함에 따라 그 차이는 적었다. 소등이 되는 동안은 점등에 비하여 상$\cdot$하층 현저한 차이를 볼 수 없고 대부분 균일하게 분포하는 양상을 보였다. 또한 실험 종료시나 24시간 주 $\cdot$야간 수직이동 시 대조구에 비하여 시험구에 분포하고 있는 C. polykrikoides의 DNA 및 RNA 변화폭이 높았다 이 실험을 통하여 담수유입은 C. polykrikoides 세포에 많은 영향을 미칠 수 있을 것으로 보이며, 특히 신속한 수직상승으로 인하여 여기에 대한 C. polykrikoides의 모니터링이 절실히 요구되는 바이다.

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